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中国应用生理学杂志 ›› 2017, Vol. 33 ›› Issue (2): 117-120.doi: 10.12047/j.cjap.5416.2017.030

• 研究论文 • 上一篇    下一篇

玉米肽联合运动对肥胖大鼠肝组织中ATGL和TNF-α影响

孙鹏, 田向阳, 史仍飞   

  1. 上海体育学院 运动科学学院, 上海 200438
  • 收稿日期:2016-01-15 修回日期:2016-11-02 出版日期:2017-03-28 发布日期:2018-06-20
  • 通讯作者: 史仍飞,Tel:021-51253247,E-mail:rfshi@sus.edu.cn E-mail:rfshi@sus.edu.cn
  • 基金资助:
    上海教委创新项目(13YZ100);上海体育学院"运动健身科技"省部共建教育部重点实验室

Effects of dietary corn peptide and exercise on hepatic ATGL and TNF-α in obese rats

SUN Peng, TIAN Xiang-yang, SHI Reng-fei   

  1. Shanghai University of Sports, College of Life Science, Shanghai 200438, China
  • Received:2016-01-15 Revised:2016-11-02 Online:2017-03-28 Published:2018-06-20
  • Supported by:
    上海教委创新项目(13YZ100);上海体育学院"运动健身科技"省部共建教育部重点实验室

摘要: 目的:探讨玉米肽结合有氧运动对肥胖大鼠体脂肪和肝细胞甘油三酯脂肪酶(ATGL)及肿瘤坏死因子α(TNF-α的)影响。方法:以32只肥胖雄性SD大鼠为实验对象,随机分为安静对照组(CON)、玉米肽组(DCP)、运动组(DEX)和玉米肽运动组(DCE)(n=8)。玉米肽组和玉米肽运动组给予玉米肽饲料,其它各组给予普通饲料。运动组和玉米肽运动组进行有氧运动,干预4周后处死取材。剥离腹腔脂肪组织和肝脏,蛋白印迹技术检测肝组织ATGL和TNF-α蛋白的表达。结果:干预4周后,与肥胖对照组相比,玉米肽运动组大鼠体重以及脂肪系数显著下降(P < 0.05,P < 0.01),且显著低于单独玉米肽补充组(P < 0.05);肝细胞ATGL蛋白表达显示,玉米肽运动组均高于其它各组,且与对照组有显著性差异(P < 0.05);玉米肽运动组肝细胞TNF-α蛋白结果均低于其它组,且显著低于对照组(P < 0.05)。结论:玉米肽结合有氧运动能有效地降低肥胖大鼠的体重以及脂肪含量,可能是玉米肽与有氧运动的协同作用,调节了肝脏ATGL和TNF-α的表达,促进脂代谢有关。

关键词: 肥胖, 玉米肽, 有氧运动, 脂肪组织脂肪酶

Abstract: Objective: To determine whether dietary corn peptides supplementation with or without exercise on body composition and fac-tors related to hepatic lipid metabolism in obese rats induced by a high fat diet. Methods: Male SD rats were fed a high-fat diet (40% calorie as fat) for 8 weeks as a dietary induced obesity model (DIO, n=32). They were then randomly divided into four groups, control, corn pip-tide(CP) group, exercise group and CP + exercise group (n=8). Supplement (CP:200 g/kg) and exercise(tread mill Ex was performed 15 m/min for 60 min/d, 5 days a week) protocol for 4 weeks. Body weight and dietary levels of rats were monitored during the experimental peri-od. At the end of the 4th weeks, rats of all groups were sacrificed. Perirenal fat and liver were harvested, and hepatic adipose triglyceride li-pase(ATGL) and TNF-α were analyzed via Western blot. Results: After 4 weeks, compared with DIO control group, body weight and perire-nal fat mass index in CP + exercise group were significantly decreased (P < 0.05). Hepatic ATGL was significantly higher in corn peptides supplementation, especially in combination with exercise (P < 0.05). At the same time, the values of TNF-α expression were decreased signif-icantly in exercise groups compared with DIO group (P < 0.05). Conclusion: Corn peptides combined with aerobic exercise has a weight re-duction by lowering adipose tissue in experimental animals, it may be related to the expression of ATGL and TNF-α in the liver, and promote lipid metabolism.

Key words: obesity, corn peptide, aerobic exercise, ATGL

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