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中国应用生理学杂志 ›› 2017, Vol. 33 ›› Issue (1): 1-5.doi: 10.12047/j.cjap.5448.2017.001

• 研究论文 •    下一篇

低氘环境对胃癌细胞生长影响的体外实验

吴涛1, 段晓剑1, 田宇1, 陈海旭2, 徐志宏3, 刘静2, 王卫华1, 朱玲玲4, 王刚石1   

  1. 1. 解放军总医院南楼消化科, 北京 100853;
    2. 解放军总医院老年医学研究所, 衰老及相关疾病研究北京重点实验室, 北京 100853;
    3. 江苏华益科技有限公司, 江苏 苏州 215500;
    4. 军事医学科学院基础医学研究所, 北京 100850
  • 收稿日期:2016-04-28 修回日期:2016-10-24 出版日期:2017-01-28 发布日期:2018-06-19
  • 通讯作者: 王刚石,Tel:010-66876245,010-66931315,E-mail:wanggangshi@hotmail.com;朱玲玲,E-mail:linglingzhu@hotmail.com E-mail:wanggangshi@hotmail.com;linglingzhu@hotmail.com
  • 基金资助:
    王刚石,Tel:010-66876245,010-66931315,E-mail:wanggangshi@hotmail.com;朱玲玲,E-mail:linglingzhu@hotmail.com

Deuterium depleted environment inhibits the growth of gastric cancer cells: in vitro study

WU Tao1, DUAN Xiao-jian1, TIAN Yu1, CHEN Hai-xu2, XU Zhi-hong3, LIU Jing2, WANG Wei-hua1, ZHU Ling-ling4, WANG Gang-shi1   

  1. 1. Department of Geriatric Gastroenterology, Chinese PLA General Hospital, Beijing 100853;
    2. Institute of Gerontology and Geriatrics, Chinese PLA General Hospital, Beijing 100853;
    3. Jiangsu Huayi Technology Co. Ltd, Suzhou 215500;
    4. Institute of Basic Medical Sciences, Beijing 100850, China
  • Received:2016-04-28 Revised:2016-10-24 Online:2017-01-28 Published:2018-06-19
  • Contact: 王刚石,Tel:010-66876245,010-66931315,E-mail:wanggangshi@hotmail.com;朱玲玲,E-mail:linglingzhu@hotmail.com E-mail:wanggangshi@hotmail.com;linglingzhu@hotmail.com
  • Supported by:
    王刚石,Tel:010-66876245,010-66931315,E-mail:wanggangshi@hotmail.com;朱玲玲,E-mail:linglingzhu@hotmail.com

摘要: 目的:研究低氘环境对人胃癌细胞(SGC-7901)增殖的影响并初步探讨其相关机制。方法:用含不同氘浓度的蒸馏水(实验组:25 ppm;对照组:150 ppm)配制的RPMI-1640培养基培养胃癌细胞SGC-7901。分别在不同的时间点对两组细胞的增殖率、细胞周期及凋亡情况进行检测,用Western blot法对两组细胞的增殖细胞核抗原蛋白(PCNA)的表达进行检测。结果:低氘环境下SGC-7901细胞的增殖率比对照组低10%左右。低氘环境对细胞的划痕愈合能力及集落形成能力也有显著抑制作用(P<0.05)。流式细胞术检测结果显示,与对照组相比,低氘组的细胞G1期细胞的比例增加(P<0.01),而其所处S期细胞的比例下降(P<0.05),两组细胞间早凋及晚凋比率差异无统计学意义。Western blot的结果显示低氘环境下培养的胃癌细胞的PCNA的表达明显下降。结论:低氘环境能够抑制胃癌细胞的生长,这可能与低氘环境下胃癌细胞阻滞于G1期及下调其PCNA的表达有关。

关键词: 低氘环境, 胃癌, 增殖, PCNA, 细胞周期, 凋亡

Abstract: Objective: To examine whether deuterium depleted environment may affect the biological features of human gastric cancer cells(SGC-7901)and explore the possible underlying mechanisms.Methods: SGC-7901 cells were cultured in RPMI-1640 medium prepared with distilled water of different deuterium concentrations(experimental group:25ppm deuterium;control group:150ppm deuterium). Assays on cellular proliferation, cell cycle and apoptosis were conducted at different time points and comparison. The protein expression of proliferating cell nuclear antigen (PCNA) was measured using Western blot.Results: In contrast to 150ppm group, the proliferation rate of SGC-7901 cells in 25ppm deuterium was decreased by 10% as indicated by the CCK-8 assay. Wound healing ability and the colony formation ability of these cells were also significantly suppressed (P<0.05). Flow cytometry analysis further revealed that exposure to 25ppm significantly increased the ratio of cancer cells at G1 phase (P<0.01) while decreased the ratio at S phase (P<0.05) compared to the 150ppm group. There was no significant difference in apoptosis between the two groups. Down-regulated expression of PCNA was also identified in cancer cells treated with 25ppm deuterium.Conclusion: Deuterium depleted environment inhibited the proliferation of gastric cancer cells, which may be attributed to the down-regulation of PCNA and cell cycle arrest at G1 phase.

Key words: deuterium depleted environment, gastric cancer, proliferation, PCNA, cell cycle, apoptosis

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