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中国应用生理学杂志 ›› 2017, Vol. 33 ›› Issue (1): 6-10.doi: 10.12047/j.cjap.5448.2017.002

• 研究论文 • 上一篇    下一篇

β3-AR阻断剂SR59230A对大鼠胸主动脉张力及microRNA表达的影响

赵倩倩, 景佳妮, 李海清, 刘蓉, 李晓鹏, 崔香丽   

  1. 山西医科大学生理学系, 细胞生理学实验室, 太原 030001
  • 收稿日期:2016-04-20 修回日期:2016-10-19 出版日期:2017-01-28 发布日期:2018-06-19
  • 通讯作者: 崔香丽,Tel:+86-0351-4135329,E-mail:cuixlcxl@sina.com E-mail:cuixlcxl@sina.com
  • 基金资助:
    山西省自然科学基金(2012201104-8);山西医科大学科技创新基金([2012]11号)

Effect of β3-AR antagonist SR59230A on the tension and microRNA expression of rat thoracic aorta

ZHAO Qian-qian, JING Jia-ni, LI Hai-qing, LIU Rong, LI Xiao-peng, CUI Xiang-li   

  1. Department of Physiology, Cell Physiology Laboratory, Shanxi Medical University, Taiyuan 030001, China
  • Received:2016-04-20 Revised:2016-10-19 Online:2017-01-28 Published:2018-06-19
  • Supported by:
    山西省自然科学基金(2012201104-8);山西医科大学科技创新基金([2012]11号)

摘要: 目的:探讨β3肾上腺素受体(β3-AR)阻断剂SR59230A (SR)对大鼠胸主动脉张力和microRNA (miRNA)表达的影响。方法:44只正常雄性SD大鼠,24只用于观察SR对离体胸主动脉环张力的影响。另20只SD大鼠随机分为对照组(control)及SR组(n=10),SR组大鼠给予SR腹腔注射,control组大鼠给予等量生理盐水腹腔注射。给药5周后2组大鼠进行无创血压测量,并检测胸主动脉环对去甲肾上腺素诱导的张力;留取2组大鼠胸主动脉组织,检测SR对大鼠胸主动脉miRNA表达的影响。结果:①SR预孵时30 mmol/L KCl引起的离体血管环收缩张力增加(P<0.05);②SR在体给药5周后大鼠收缩压升高(P<0.05);③SR在体给药后去甲肾上腺素(NA)浓度为1μmol/L和10μmol/L时诱导大鼠胸主动脉环张力增加(P<0.05,P<0.01);④SR组大鼠胸主动脉18个miRNA表达下调,其中7个有统计学意义,分别是rno-miR-143-3p、rno-miR-29b-3p、rno-miR-31a-5p、rno-let-7b-5p、rno-miR-214-3p、rno-miR-222-3p和rno-miR-352;11个表达上调,其中4个有统计学意义,分别是rno-miR-206-3p、rno-miR-223-3p、rno-miR-342-3p、rno-miR-499-5p。结论:SR59230A可引起大鼠胸主动脉血管张力增加,在体给药后可使大鼠收缩压升高及胸主动脉rno-miR-143-3p、rno-miR-29b-3p、rno-miR-31a-5p、rno-let-7b-5p、rno-miR-214-3p、rno-miR-222-3p、rno-miR-352表达下调和rno-miR-206-3p、rno-miR-223-3p、rno-miR-342-3p、rno-miR-499-5p表达上调。

关键词: 大鼠, SR59230A, 胸主动脉, 张力, microRNA

Abstract: Objective: To explore the effects of SR59230A on the tension and microRNA (miRNA) expression of rat thoracic aorta.Methods: Forty-four SD rats were used in the experiment. Twenty-four rats were used to observe the effect of SR on the tension of thoracic aortic rings. Another 20 rats were randomly divided into control (n=10) and SR group(n=10). Rats in SR group were injected SR intraperitoneally,and in control group were given 0.9% of saline. After 5 weeks, the blood pressure of all rats were measured. Then the tension to NA and the expression of miRNA of thoracic aorta rings were measured.Results: (1) The tension of thoracic aortic rings responding to 30 mmol/LKCl were increased by pretreatment of SR (P<0.05); (2) After 5 weeks injection of SR, systolic pressure was increased (P<0.05); (3) The tension in SR group was increased in presence of 1 μmol/L and 10 μmol/L of NA (P<0.05,P<0.01). (4) After 5 weeks of SR in vivo application,18 miRNA were down-regulated, 7 of them had statistical significance, they were rno-miR-143-3p, rno-miR-29b-3p, rno-miR-31a-5p, rno-let-7b-5p, rno-miR-214-3p, rno-miR-222-3p and rno-miR-352; 11 miRNA were up-regulated, 4 of them had statistical significance, they were rno-miR-206-3p、rno-miR-223-3p、rno-miR-342-3p and rno-miR-499-5p respectively.Conclusion: SR59230A increased the tension of rat thoracic aorta. In vivo administration of SR led to increase of systolic pressure of rat,down-regulation of rno-miR-143-3p、rno-miR-29b-3p、rno-miR-31a-5p、rno-let-7b-5p、rno-miR-214-3p、rno-miR-222-3p、rno-miR-352 and up-regulation of rno-miR-206-3p、rno-miR-223-3p、rno-miR-342-3p and rno-miR-499-5p.

Key words: rat, SR59230A, thoracic aorta, tension, microRNA

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