不同温度刺激与大气PM2.5对大鼠肺泡巨噬细胞的交互毒性作用研究

doi:10.12047/j.cjap.5448.2017.018

中国应用生理学杂志 ›› 2017, Vol. 33 ›› Issue (1): 71-75.doi: 10.12047/j.cjap.5448.2017.018

不同温度刺激与大气PM2.5对大鼠肺泡巨噬细胞的交互毒性作用研究

罗斌, 刘江涛, 费高强, 韩婷, 王丽娜, 晚亚雄, 张凯, 王任洪

兰州大学公共卫生学院劳动卫生与环境卫生研究所, 甘肃兰州 730000

收稿日期:2016-03-14 修回日期:2016-10-26 出版日期:2017-01-28 发布日期:2018-06-19
通讯作者: 罗斌,Tel:13919783313,E-mail:luob@lzu.edu.cn E-mail:luob@lzu.edu.cn
基金资助:
国家自然科学基金资助项目（41405108）；兰州大学中央高校基本科研业务费专项资金项目（lzujbky-2015-181）；兰州大学"本科教学工程"国家级大学生创新创业训练计划（201510730159）

Impact of different temperatures and ambient PM2.5 on the cytotoxicity and cytophagocytic function of alveolar macrophages of rat

LUO Bin, LIU Jiang-tao, FEI Gao-qiang, HAN Ting, WANG Li-na, WAN Ya-xiong, ZHANG Kai, WANG Ren-hong

Institute of Occupational Health and Environmental Health, School of Public Health, Lanzhou University, Lanzhou 730000, China

Received:2016-03-14 Revised:2016-10-26 Online:2017-01-28 Published:2018-06-19
Supported by:
国家自然科学基金资助项目（41405108）；兰州大学中央高校基本科研业务费专项资金项目（lzujbky-2015-181）；兰州大学"本科教学工程"国家级大学生创新创业训练计划（201510730159）

摘要/Abstract

摘要： 目的：探究不同温度刺激与大气PM2.5对大鼠肺泡巨噬细胞的交互毒性作用。方法：提取大鼠肺泡巨噬细胞，分别在18℃、24℃、30℃、37℃、43℃下培养，每个温度组下设置不同浓度PM2.5处理，依次为100μg/ml、50μg/ml、25μg/ml、0μg/ml。处理8 h后，观察两因素对大鼠肺泡巨噬细胞组织形态学、细胞存活率和吞噬功能的影响。结果：同一温度下，不同剂量PM2.5组的相对细胞存活率和吞噬功能均显著低于空白对照组（P<0.05），且剂量越高，毒性作用越强，吞噬功能越弱。而在各温度组间，37℃组相对细胞存活率和吞噬功能最高，而随着温度的升高或降低，相对细胞存活率降低，吞噬功能下降。结论：不同温度刺激和大气PM2.5处理均会对大鼠肺泡巨噬细胞产生毒性作用，使其吞噬能力降低，并且温度与37℃差距越大、PM2.5浓度越高时毒性作用越明显。

关键词: 不同温度刺激, 大气PM2.5, 肺泡巨噬细胞, 交互毒性作用

Abstract: Objective: To investigate the interactive effects of different temperatures and ambient PM2.5 on the rat alveolar macrophages.Methods: The rat alveolar macrophages were collected. The cells were exposed in vitro to 18℃, 24℃, 30℃, 37℃ and 43℃ with PM2.5 at the concentrations of 100 μg/ml, 50 μg/ml, 25 μg/ml and 0 μg/ml respectively. The cells were cultured in the different cases for 8 hours, then cytotoxicity was assessed by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT)reduction assay and phagocytosis function of macrophages was assessed by neutral red absorption test.Results: The relative survival rate and the cytophagocytic function of alveolar macrophages of rats among the different concentration groups decreased significantly (P<0.05) compared with the blank control group. Both were dose-dependent. The 37℃ group had the highest level of relative survival rate and the cytophagocytic function compared with other different temperatures groups. Interactive effect of different temperatures and ambient PM2.5 was not observed. But the lower temperature and the higher PM2.5 concentration group had stronger toxicity to alveolar macrophages.Conclusion: The results suggested that different temperatures and ambient PM2.5 have cytotoxicity on alveolar macrophages,injuring the phagocytosis. The two factors had some interaction.

Key words: different temperatures, ambient PM2.5, cytotoxicity, cytophagocytic, macrophages

罗斌, 刘江涛, 费高强, 韩婷, 王丽娜, 晚亚雄, 张凯, 王任洪. 不同温度刺激与大气PM2.5对大鼠肺泡巨噬细胞的交互毒性作用研究[J]. 中国应用生理学杂志, 2017, 33(1): 71-75.

LUO Bin, LIU Jiang-tao, FEI Gao-qiang, HAN Ting, WANG Li-na, WAN Ya-xiong, ZHANG Kai, WANG Ren-hong. Impact of different temperatures and ambient PM2.5 on the cytotoxicity and cytophagocytic function of alveolar macrophages of rat[J]. CJAP, 2017, 33(1): 71-75.

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不同温度刺激与大气PM2.5对大鼠肺泡巨噬细胞的交互毒性作用研究

Impact of different temperatures and ambient PM2.5 on the cytotoxicity and cytophagocytic function of alveolar macrophages of rat

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