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中国应用生理学杂志 ›› 2017, Vol. 33 ›› Issue (1): 78-82.doi: 10.12047/j.cjap.5448.2017.020

• 研究论文 • 上一篇    下一篇

长链非编码RNA SPRY4-IT1对髓母细胞瘤增殖及侵袭转移的影响

史鹏飞1, 吉海龙2, 罗永康2, 毛天明2, 陈茜3, 周开宇1   

  1. 1. 温州医科大学附属第一临床医学院, 浙江温州 325000;
    2. 浙江省台州市立医院神经外科, 台州 318000;
    3. 浙江省台州市立医院病理科, 台州 318000
  • 收稿日期:2016-03-28 修回日期:2016-10-24 出版日期:2017-01-28 发布日期:2018-06-19
  • 通讯作者: 周开宇,Tel:13957680507,E-mail:kerry2000year@163.com E-mail:kerry2000year@163.com
  • 基金资助:
    浙江省中医药管理局科技计划资助项目(2013ZA133,2015ZB133);台州市科技局科技计划资助项目(14SF05)

Effect of long noncoding RNA SPRY4-IT1 on proliferation and metastasis of medulloblastoma

SHI Peng-fei1, JI Hai-long2, LUO Yong-kang2, MAO Tian-ming2, CHEN Xi3, ZHOU Kai-yu1   

  1. 1. the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000;
    2. Department of Neurosurgery, Zhejiang Taizhou Municipal Hospital, Taizhou 318000, China;
    3. Department of Pathology, Zhejiang Taizhou Municipal Hospital, Taizhou 318000, China
  • Received:2016-03-28 Revised:2016-10-24 Online:2017-01-28 Published:2018-06-19
  • Supported by:
    浙江省中医药管理局科技计划资助项目(2013ZA133,2015ZB133);台州市科技局科技计划资助项目(14SF05)

摘要: 目的:探讨长链非编码RNA SPRY4-IT1(LncRNA)对髓母细胞瘤增殖及侵袭转移的影响。方法:髓母细胞瘤Daoy细胞分为对照组和si-SPRY4-IT1组,分别利用脂质体Lipofectamine 2000将阴性对照荧光序列和SPRY4-IT1-siRNA转入细胞中,采用Real-time PCR检测转染后各组细胞中SPRY4-IT1的表达情况,CCK-8实验及平板克隆形成实验检测细胞增殖能力的变化,以细胞体外侵袭、迁移实验分别检测细胞侵袭、迁移能力的变化,Western blot检测SPRY4-IT1对基质金属蛋白酶(MMP)-2和MMP-9蛋白的影响。结果:si-SPRY4-IT1组SPRY4-IT1 mRNA表达水平、细胞体外增殖能力、细胞侵袭、迁移能力、细胞MMP-2蛋白表达均较对照组明显降低(P<0.05),而MMP-9蛋白表达未见明显变化。结论:干扰长链非编码RNA SPRY4-IT1在髓母细胞瘤Daoy细胞中的表达能显著抑制细胞的增殖、侵袭和迁移能力。

关键词: 髓母细胞瘤, 长链非编码RNA(LncRNA), SPRY4-IT1, 增殖, 侵袭迁移

Abstract: Objective: To investigate the effects of long non-coding RNA SPRY4-IT1 (LncRNA) on proliferation and metastasis of medul-loblastoma cells.Methods: SPRY4-IT1siRNA and control fluorescence siRNA were transfected into medulloblastoma cell line Daoy with Lipo-fe ctamine 2000 and were divided into control group and si-SPRY4-IT1 group. Relative expression of SPRY4-IT1 mRNA were detected by real-time quantitative PCR. The change of cell proliferation were examined using CCK-8 kit and clone forming experiment. The change of cell inva-sion and metastasis were examined by matrigel invasion assay and cell metastasis experiment respectively, The expression of matrix metallopro-teinase MMP-2 and MMP-9 were detected by Western blot.Results: In si-SPRY4-IT1 group,the SPRY4-IT1 mRNA expression level, cell pro-liferation in vitro,cell invasion and migration ability, MMP-2 protein expression were significantly lower than those in the control group.Conclusion: Interference SPRY4-IT1 expression has prominent inhibitory effect on the cell proliferation、invasion and metastasis of medulloblastoma cell line Daoy.

Key words: medulloblastoma, long noncoding RNA (LncRNA), SPRY4-IT1, proliferation, invasion and metastasis

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