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中国应用生理学杂志 ›› 2017, Vol. 33 ›› Issue (5): 393-397.doi: 10.12047/j.cjap.5473.2017.095

• 研究论文 • 上一篇    下一篇

JAK2/STAT3信号通路在运动预适应抗心肌细胞凋亡中的作用

孙晓娟, 冯武龙, 侯娜   

  1. 咸阳师范学院体育学院, 陕西 咸阳 712000
  • 收稿日期:2016-07-13 修回日期:2017-05-17 出版日期:2017-09-28 发布日期:2018-06-19
  • 通讯作者: 孙晓娟,Tel:15991039053;E-mail:565489181@qq.com E-mail:565489181@qq.com
  • 基金资助:
    陕西省教育厅科学研究计划项目(16JK1830);2016咸阳师范学院国家级大学生创新创业训练计划资助项目(201610722029)

The role of JAK2/STAT3 signaling pathway in exercise preconditioning against myocardial apoptosis

SUN Xiao-juan, FENG Wu-long, HOU Na   

  1. School of Sport, Xianyang Normal University, Xianyang 712000, China
  • Received:2016-07-13 Revised:2017-05-17 Online:2017-09-28 Published:2018-06-19
  • Supported by:
    陕西省教育厅科学研究计划项目(16JK1830);2016咸阳师范学院国家级大学生创新创业训练计划资助项目(201610722029)

摘要: 目的:探讨Janus激酶2-信号转导子和转录激活子3(JAK2/STAT3)信号通路在运动预适应(EP)抗心肌细胞凋亡中的作用及其机制。方法:健康雄性SD大鼠80只,随机分为对照组(C组)、力竭组(EE组)、运动预适应组(EP组)、运动预适应+AG490组(EP+AG组)(n=20)。连续3 d的间歇跑台运动建立EP动物模型,力竭运动致大鼠运动性心肌损伤。采用TUNEL法检测心肌细胞凋亡改变、Western blot法检测心脏Caspase-3定量表达的变化,免疫组织化学法和Western blot法显示心脏p-JAK2和p-STAT3定位和定量表达的变化。结果:与C组相比,EE组心肌细胞凋亡、心脏Caspase-3、p-JAK2和p-STAT3的表达均显著升高;与EE组相比,EP组心肌细胞凋亡和心脏Caspase-3表达明显降低,而心脏p-JAK2和p-STAT3表达显著升高;与EP组相比,EP+AG组心肌细胞凋亡和心脏Caspase-3表达均显著升高,而心脏p-JAK2和p-STAT3表达明显降低。结论:EP可诱导心脏磷酸化JAK2和STAT3表达增加,减少心脏Caspase-3的表达,抑制心肌细胞凋亡,提示JAK2/STAT3信号通路参与了EP抗心肌细胞凋亡的作用。

关键词: JAK2/STAT3通路, 运动预适应, 力竭运动, 心肌细胞凋亡, 大鼠

Abstract: Objective: To study the effects of Janus kinase 2/signal transducers and activators of transcription 3 (JAK2/STAT3) in exercise preconditioning (EP) against myocardial apoptosis.Methods: Eighty healthy male SD rats were randomly divided into control group(C), exercise exhaust(EE) group, EP group, and EP+JAK2 inhibitor AG490(AG) group(n=20). By using 3 days intermittent treadmill exercise, the EP animal model was established, and myocardial injury was induced by exhaustive exercise on treadmill. The changes of myocardial apoptosis were evaluated by TUNEL. The expressions of Caspase-3, p-JAK2 and p-STAT3 in heart were detected by Western blot or immunohistochemistry.Results: Compared with group C, myocardial apoptosis, and the expressions of Caspase-3, p-JAK2, and p-STAT3 in heart were increased significantly in group EE. Compared with group EE, myocardial apoptosis and the expression of Caspase-3 were decreased significantly, while the expressions of p-JAK2 and p-STAT3 were increased significantly in group EP. Compared with group EP, myocardial apoptosis and the expression of Caspase-3 were increased significantly, while the expressions of p-JAK2 and p-STAT3 were decreased significantly in group EP+AG.Conclusion: EP could increase the expressions of p-JAK2 and p-STAT3 and decrease the expression of Caspase-3 in heart, which further mitigate myocardial apoptosis. Hence, JAK2/STAT3 pathway may participate in EP against myocardial apoptosis.

Key words: JAK2/STAT3 pathway, exercise preconditioning, exhaustive exercise, myocardial apoptosis, rat

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