模拟100 m氦氮氧常规潜水对家兔氧化应激和炎性反应的影响

doi:10.12047/j.cjap.5484.2018.001

中国应用生理学杂志 ›› 2018, Vol. 34 ›› Issue (1): 1-3.doi: 10.12047/j.cjap.5484.2018.001

• 研究论文 • 下一篇

模拟100 m氦氮氧常规潜水对家兔氧化应激和炎性反应的影响

李洋洋¹, 石路^2,3, 张延猛², 肖婵娟¹, 刘洪涛¹

1. 上海交通大学海洋水下工程科学研究院有限公司, 上海 200231;
2. 上海交通大学海洋水下工程科学研究院, 上海 200231;
3. 上海交通大学-千叶大学国际合作研究中心, 上海 200231

收稿日期:2016-10-18 修回日期:2017-09-19 出版日期:2018-01-28 发布日期:2018-06-19
通讯作者: 石路,Tel:13701696884;E-mail:shilu@sjtu.edu.cn E-mail:shilu@sjtu.edu.cn
基金资助:
中华人民共和国科学技术部专项基金（2012EG124043）

Effects of simulated 100 m Trimix conventional diving on oxidative stress and inflammatory reaction in rabbits

LI Yang-yang¹, SHI Lu^2,3, ZHANG Yan-meng², XIAO Chan-juan¹, LIU Hong-tao¹

1. Shanghai Jiao Tong University Underwater Engineering Insititute Co. Ltd, Shanghai 200231, China;
2. Shanghai Jiao Tong University, Institute of Underwater Technology, Shanghai 200231, China;
3. Shanghai Jiao Tong University-Chiba University International Cooperative Research Center, Shanghai 200231, China

Received:2016-10-18 Revised:2017-09-19 Online:2018-01-28 Published:2018-06-19
Supported by:
中华人民共和国科学技术部专项基金（2012EG124043）

摘要/Abstract

摘要： 目的：探讨模拟100 m氦氮氧（Trimix）常规潜水对家兔氧化应激和炎性反应的影响。方法：空白组家兔8只，模拟100 m Trimix；常规潜水组家兔8只。模拟潜水按照Haldane理论计算所得的水下阶段减压表减压。Elisa法检测家兔模拟潜水前、后血清超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、过氧化氢酶（CAT）、丙二醛（MDA）和过氧化脂质（LPO）等氧化/抗氧化指标的变化。同样采用Elisa法检测空白组和潜水组家兔肺、脑组织γ干扰素（IFN-γ）、肿瘤坏死因子-α（TNF-α）、白介素6（IL-6）、白介素8（IL-8）、髓过氧化物酶活力（MPO）和基质金属蛋白酶9（MMP-9）等炎性因子的表达，并比较组织湿干比（Wet/Dry ratio）。结果：家兔潜水后SOD和GSH活性明显降低（P<0.01），CAT、MDA和LPO含量明显升高（P<0.01）；潜水组家兔肺、脑组织中IL-8、IL-6、IFN-γ、TNF-α、MMP-9和MPO的表达均较空白组显著升高（P<0.05，P<0.01）；潜水组家兔肺、脑组织湿干比与空白组差异不显著。结论：模拟100 m Trimix常规潜水对家兔氧化应激和炎性反应有显著影响。

关键词: 氦氮氧常规潜水, 炎性反应, 氧化应激, 湿干比

Abstract: Objective: To investigate the effects of simulated 100 m Trimix conventional diving on tissue inflammatory cytokines in rabbits.Methods: Eight New Zealand rabbits were performed a simulated 100 m Trimix conventional diving program which was established according to the Haldane theory. The expression levels of interferon-gamma(IFN-γ), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-8 (IL-8), myeloperoxidase(MPO) and matrix metallo proteinase-9 (MMP-9) in rabbits lung and brain tissues were detected by Elisa after diving decompression. The tissue wet/dry ratio was calculated. The serum levels of superoxide dismutase (SOD),glutathione(GSH), catalase(CAT), malondiadehyde(MDA) and lipid peroxide(LPO) were detected by Elisa method in rabbits before and after diving.Results: The expressions of IFN-γ, TNF-α, IL-6, IL-8, MPO and MMP-9 in simulated diving group rabbits were significantly increased compared with the intact group(P<0.05, P<0.01); the simulated diving rabbits tissues wet/dry ratio had no significant changes compared with the intact group. After diving, the activities of SOD and GSH were decreased significantly (P<0.01), while the contents of CAT, MDA and LPO were increased significantly (P<0.01).Conclusion: The simulated 100 m Trimix conventional diving had significant impact on oxidative stress and inflammatory reaction in rabbits, the results of wet/dry ratio showed that the diving rabbits had no tissue edema after decompression.

Key words: Trimix conventional diving, inflammatory reaction, oxidative stress, wet/dry ratio

李洋洋, 石路, 张延猛, 肖婵娟, 刘洪涛. 模拟100 m氦氮氧常规潜水对家兔氧化应激和炎性反应的影响[J]. 中国应用生理学杂志, 2018, 34(1): 1-3.

LI Yang-yang, SHI Lu, ZHANG Yan-meng, XIAO Chan-juan, LIU Hong-tao. Effects of simulated 100 m Trimix conventional diving on oxidative stress and inflammatory reaction in rabbits[J]. CJAP, 2018, 34(1): 1-3.

参考文献

[1] Schwerzmann M, Seiler C. Recreational scuba diving, patent foramen ovale and their associated risks[J]. Swiss Med Wkly, 2001, 131(25-26):365-374.
[2] Boussuges A. A rat model to study decompression sickness after a trimix dive[J]. J Appl Physiol, 2007, 102(4):1301-1302.
[3] Levett DZH, Millar IL. Bubble trouble:a review of diving physiology and disease[J]. Postgrad Med J, 2008, 84(997):571-578.
[4] Arieli R, Ertracht O, Oster I, et al. Effects of nitrogen and helium on CNS oxygen toxicity in the rat[J]. J Appl Physiol, 2005, 98(1):144-150.
[5] Rostain JC, Dumas JC, Gardette B, et al. Effects of addition of nitrogen during rapid compression of baboons[J]. J Appl Physiol Respir Environ Exerc Physiol, 1984, 57(2):332-340.
[6] Bennett PB, Coggin R, Roby J. Control of HPNS in humans during rapid com pre ssion with trimix to 650 m (2131 ft)[J]. Undersea Biomed Res, 1981, 8(2):85-100.
[7] 李洋洋, 石路, 张延猛, 等. 模拟100 m氦氮氧常规潜水水下阶段减压方案的计算与动物实验验证[J]. 中国应用生理学杂志, 2015, 31(3):197-200.
[8] 李慈, 何佳, 刘民航, 等. 模拟65 msw氦氧饱和潜水对人体氧化应激的影响[J]. 中国应用生理学杂志, 2011(2):250-252.
[9] Bitterman N, Bitterman H, Kinarty A, et al. Effect of a single expos ure to hyperbaric oxygen on blood mononuclear cells in human subjects[J]. Undersea H yperb Med, 1993, 20(3):197-204.
[10] Bitterman N, Lahat N, Rosenwald T, et al. Effect of hyperbar ic oxygen on ti ssue distribution of mononuclear cell subsets in the rat[J]. J Appl Physiol, 1994, 77(5):2355-2359.
[11] Allan IM, Lunec J, Salmon M, et al. Reactive oxygen species selectively dep lete normal T lymphocytes via a hydroxyl radical dependent mechanism[J]. Sca nd J Immunol, 1987, 26(1):47-53.
[12] Bose KS, Vyas P, Singh M. Plasma non-enzymatic antioxidants-vitamin C, E, beta-carotenes, reduced glutathione levels and total antioxidant activity in or a l sub mucous fibrosis[J]. Eur Rev Med Pharmacol Sci, 2012, 16(4):530-532.

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模拟100 m氦氮氧常规潜水对家兔氧化应激和炎性反应的影响

Effects of simulated 100 m Trimix conventional diving on oxidative stress and inflammatory reaction in rabbits

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