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中国应用生理学杂志 ›› 2018, Vol. 34 ›› Issue (1): 1-3.doi: 10.12047/j.cjap.5484.2018.001

• 研究论文 •    下一篇

模拟100 m氦氮氧常规潜水对家兔氧化应激和炎性反应的影响

李洋洋1, 石路2,3, 张延猛2, 肖婵娟1, 刘洪涛1   

  1. 1. 上海交通大学海洋水下工程科学研究院有限公司, 上海 200231;
    2. 上海交通大学海洋水下工程科学研究院, 上海 200231;
    3. 上海交通大学-千叶大学国际合作研究中心, 上海 200231
  • 收稿日期:2016-10-18 修回日期:2017-09-19 出版日期:2018-01-28 发布日期:2018-06-19
  • 通讯作者: 石路,Tel:13701696884;E-mail:shilu@sjtu.edu.cn E-mail:shilu@sjtu.edu.cn
  • 基金资助:
    中华人民共和国科学技术部专项基金(2012EG124043)

Effects of simulated 100 m Trimix conventional diving on oxidative stress and inflammatory reaction in rabbits

LI Yang-yang1, SHI Lu2,3, ZHANG Yan-meng2, XIAO Chan-juan1, LIU Hong-tao1   

  1. 1. Shanghai Jiao Tong University Underwater Engineering Insititute Co. Ltd, Shanghai 200231, China;
    2. Shanghai Jiao Tong University, Institute of Underwater Technology, Shanghai 200231, China;
    3. Shanghai Jiao Tong University-Chiba University International Cooperative Research Center, Shanghai 200231, China
  • Received:2016-10-18 Revised:2017-09-19 Online:2018-01-28 Published:2018-06-19
  • Supported by:
    中华人民共和国科学技术部专项基金(2012EG124043)

摘要: 目的:探讨模拟100 m氦氮氧(Trimix)常规潜水对家兔氧化应激和炎性反应的影响。方法:空白组家兔8只,模拟100 m Trimix;常规潜水组家兔8只。模拟潜水按照Haldane理论计算所得的水下阶段减压表减压。Elisa法检测家兔模拟潜水前、后血清超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、过氧化氢酶(CAT)、丙二醛(MDA)和过氧化脂质(LPO)等氧化/抗氧化指标的变化。同样采用Elisa法检测空白组和潜水组家兔肺、脑组织γ干扰素(IFN-γ)、肿瘤坏死因子-α(TNF-α)、白介素6(IL-6)、白介素8(IL-8)、髓过氧化物酶活力(MPO)和基质金属蛋白酶9(MMP-9)等炎性因子的表达,并比较组织湿干比(Wet/Dry ratio)。结果:家兔潜水后SOD和GSH活性明显降低(P<0.01),CAT、MDA和LPO含量明显升高(P<0.01);潜水组家兔肺、脑组织中IL-8、IL-6、IFN-γ、TNF-α、MMP-9和MPO的表达均较空白组显著升高(P<0.05,P<0.01);潜水组家兔肺、脑组织湿干比与空白组差异不显著。结论:模拟100 m Trimix常规潜水对家兔氧化应激和炎性反应有显著影响。

关键词: 氦氮氧常规潜水, 炎性反应, 氧化应激, 湿干比

Abstract: Objective: To investigate the effects of simulated 100 m Trimix conventional diving on tissue inflammatory cytokines in rabbits.Methods: Eight New Zealand rabbits were performed a simulated 100 m Trimix conventional diving program which was established according to the Haldane theory. The expression levels of interferon-gamma(IFN-γ), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-8 (IL-8), myeloperoxidase(MPO) and matrix metallo proteinase-9 (MMP-9) in rabbits lung and brain tissues were detected by Elisa after diving decompression. The tissue wet/dry ratio was calculated. The serum levels of superoxide dismutase (SOD),glutathione(GSH), catalase(CAT), malondiadehyde(MDA) and lipid peroxide(LPO) were detected by Elisa method in rabbits before and after diving.Results: The expressions of IFN-γ, TNF-α, IL-6, IL-8, MPO and MMP-9 in simulated diving group rabbits were significantly increased compared with the intact group(P<0.05, P<0.01); the simulated diving rabbits tissues wet/dry ratio had no significant changes compared with the intact group. After diving, the activities of SOD and GSH were decreased significantly (P<0.01), while the contents of CAT, MDA and LPO were increased significantly (P<0.01).Conclusion: The simulated 100 m Trimix conventional diving had significant impact on oxidative stress and inflammatory reaction in rabbits, the results of wet/dry ratio showed that the diving rabbits had no tissue edema after decompression.

Key words: Trimix conventional diving, inflammatory reaction, oxidative stress, wet/dry ratio

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