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中国应用生理学杂志 ›› 2017, Vol. 33 ›› Issue (5): 405-409.doi: 10.12047/j.cjap.5498.2017.098

• 研究论文 • 上一篇    下一篇

脂联素改善阿尔茨海默病模型小鼠焦虑情绪及工作记忆

曲雪松1, 石辉1,2, 曹秀丽1, 董雪帆1, 李甜1, 焦娟娟1, 祁金顺1, 武美娜1   

  1. 1. 山西医科大学生理学系, 细胞生理学省部共建教育部重点实验室, 太原 030001;
    2. 内蒙古赤峰市医院重症医学科, 赤峰 024000
  • 收稿日期:2016-10-08 修回日期:2017-05-22 出版日期:2017-09-28 发布日期:2018-06-19
  • 通讯作者: 武美娜,Tel:0351-4135117;E-mail:wmna@163.com E-mail:wmna@163.com
  • 基金资助:
    国家自然科学基金(31300968);青年三晋学者支持计划专项经费资助

Effects of adiponectin on the anxiety and memory impairment in triple transgenic Alzheimer's disease model mice

QU Xue-song1, SHI Hui1,2, CAO Xiu-li1, DONG Xue-fan1, LI Tian1, JIAO Juan-juan1, QI Jin-shun1, WU Mei-na1   

  1. 1. Department of Physiology, Shanxi Medical University, Key Laboratory for Cellular Physiology of Ministry of Education, Taiyuan 030001;
    2. Intensive Care Unit, Chifeng Municipal Hospital of Inner Mongolia, Chifeng 024000, China
  • Received:2016-10-08 Revised:2017-05-22 Online:2017-09-28 Published:2018-06-19
  • Supported by:
    国家自然科学基金(31300968);青年三晋学者支持计划专项经费资助

摘要: 目的:探讨脂联素(APN)预处理对9月龄三转基因阿尔茨海默病(3xTg-AD)模型小鼠学习记忆能力和焦虑情绪的影响。方法:选取9月龄3xTg-AD小鼠及C57BL/6J小鼠,分为4组:WT+Saline组、3xTg-AD+Saline组、WT+APN组和3xTg-AD+APN组,每组8只。将全部小鼠进行侧脑室埋管术后,恢复7 d,在自由清醒状态下分别经侧脑室给予生理盐水或APN,采用旷场、新物体识别及Y-迷宫3种行为学手段检测小鼠的情绪及学习记忆能力。结果:①在旷场实验中,与WT+Saline组小鼠相比,3xTg-AD+Saline组小鼠在中央区域的活动时间明显缩短,在外周区域的活动时间明显延长,给予APN后可有效逆转3xTg-AD小鼠的该现象,表明脂联素可有效缓解3xTg-AD小鼠的焦虑情绪。②新物体识别实验中,3xTg-AD+Saline组小鼠的辨别指数为(-16.7±10.1)%,明显低于WT+Saline组的(18.0±8.2)%(P<0.01)和3xTg-AD+APN组的(15.7±8.8)%(P<0.01),表明脂联素可明显改善3xTg-AD小鼠的识别记忆能力损伤。③Y-迷宫实验中,3xTg-AD+Saline组小鼠的自发交替正确率为(40.0±1.7)%,明显低于WT+Saline组的(56.6±4.6)%(P<0.01)和3xTg-AD+APN组的(53.9±5.6)%(P<0.01),表明脂联素能够逆转3xTg-AD小鼠工作记忆能力的损伤。结论:脂联素可以改善9月龄3xTg-AD小鼠的焦虑情绪及识别记忆和工作记忆能力损伤,可能在AD的预防和治疗中发挥有效作用。

关键词: 脂联素, 阿尔茨海默病, 三转基因小鼠, 焦虑, 学习记忆

Abstract: Objective: To investigate the effects of adiponectin (APN) on anxiety and memory impairment of 9-month-old triple transgenic Alzheimer's disease (3xTg-AD) model mice.Methods: The 9-month-old 3xTg-AD mice and C57BL/6J mice were randomly divided into four groups (n=8 for each group):Wild type(WT)+Saline, 3xTg-AD +Saline, WT+APN and 3xTg-AD +APN group. All mice were implanted cannula in lateral ventricle and each mouse was intracerebroventricular injected with adiponectin or saline under free moving condition after 7 days recovery. The anxiety and memory ability of each mouse were observed by using open field test, object recognition task and Y-maze test.Results: ①In the open field test, compared to WT+Saline group, the time of 3xTg-AD +Saline mice spent in center was significantly decreased, and the time spent in periphery was obviously increased. However, APN treatment effectively reversed the phenomenon appeared in 3xTg-AD mice, indicating that APN could alleviate the anxiety observed in 3xTg-AD mice. ②In novel object recognition task, the discrimination index of 3xTg-AD+Saline group was (-16.7±10.1)%, significantly lower than (18.0±8.2)% in WT+Saline group (P<0.01) and (15.7±8.8)% in 3xTg-AD+APN group (P<0.01), which indicated that APN could effectively prevent the recognition memory impairment in 3xTg mice. ③In Y-maze test, the spontaneous alternation rate of 3xTg-AD +Saline group was (40.0±1.7)%,significantly lower than (56.6±4.6)% in WT+Saline group and (53.9±5.6)% in 3xTg-AD +APN group (P<0.01), which indicated that APN could prevent working memory impairment in 3xTg-AD mice.Conclusion: Adiponectin could effectively alleviate the anxiety and reverse the impairment of recognition memory and working memory of 9-month-old 3xTg-AD mice, and might play an important role in the prevention and treatment of AD.

Key words: adiponectin, Alzheimer's disease (AD), triple-transgenic AD (3xTg-AD) mice, anxiety, learning and memory

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