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中国应用生理学杂志  2017, Vol. 33 Issue (5): 466-469    DOI: 10.12047/j.cjap.5535.2017.111
  研究论文 本期目录 | 过刊浏览 | 高级检索 |
葛根素对人非小细胞肺癌A549细胞凋亡的作用
金鑫1, 孙盼盼2, 洪云3, 虞乐4, 李曙4
1. 皖南医学院药理学教研室, 芜湖 241000;
2. 皖南医学院护理学教研室, 芜湖 241000;
3. 皖南医学院弋矶山医院超声医学科, 芜湖 241000;
4. 皖南医学院病理生理学教研室, 芜湖 241000
Puerarin induces apoptosis in A549 cells
JIN Xin1, SUN Pan-pan2, HONG Yun3, YU Le4, LI Shu4
1. Department of Pharmacology, Wannan Medical College, Wuhu 241002, China;
2. Department of Nursing, Wannan Medical College, Wuhu 241002, China;
3. Department of Ultrasonography, Yijishan Hospital, Wuhu 241002, China;
4. Department of Pathophysiology, Wannan Medical College, Wuhu 241002, China
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摘要 目的:观察葛根素对人非小细胞肺癌A549细胞生长的抑制作用及其机制。方法:体外培养人非小细胞肺癌细胞(A549),不同浓度(60 μg/ml,120 μg/ml,240 μg/ml)葛根素处理24 h后;采用CCK-8法观察葛根素对细胞的增值抑制作用;吖啶橙(AO)/溴化乙锭(EB)双染法及AnnexinⅤ-PI双染流式细胞术检测药物作用前后A549细胞的形态学变化及凋亡状况;Western blot法检测Apelin/APJ蛋白水平的变化。结果:CCK-8法检测结果说明葛根素能抑制A549细胞的增值,具有浓度和时间依赖关系;流式细胞术进一步证实葛根素具有诱导细胞凋亡的作用,与A549细胞组比较,葛根素各处理组Apelin/APJ蛋白水平均有不同程度下调。结论:葛根素可能通过调节Apelin/APJ蛋白的表达诱导A549细胞凋亡。
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金鑫
孙盼盼
洪云
虞乐
李曙
关键词 葛根素A549Apelin/APJ凋亡    
AbstractObjective: To explore the effect of puerarin induced apoptosis in human non-small cell lung canner A549 cells and its possible mechanisms.Methods: A549 cells were cultivated in vitro. When 80%~90% confluence was reached, they were treated with puerarin in different concentration (60、120、240 μg/ml). The effects on cell apoptpsis were examined by CCK-8 colorimetry. Characteristic morphological changes of apoptosis were observed by AO/EB staining. The apoptosis of A549 cells were detected by flow cytometry analysis. The proteins level of Apelin and APJ were tested by Western blot.Results: CCK-8 test showed that puerarin could inhibit the proliferation of A549 cells in a time and concentration dependent manner. Flow cytometry analysis indicated that puerarin could make A549 cells apoptosis. Compared with the A549 cells group, the protein expression of Apelin/APJ was decreased in puerarin groups.Conclusion: Puerarin induces apoptosis in A549 cells, and its mechanism may through the regulation of Apelin/APJ expression.
Key wordspuerarin    A-549    Apelin/APJ    apoptosis
收稿日期: 2016-12-09      出版日期: 2018-06-19
基金资助:皖南医学院校级重点科研项目培育基金(WK2017Z04)
通讯作者: 李曙,Tel:13965183595;E-mail:258104975@qq.com     E-mail: 258104975@qq.com
引用本文:   
金鑫, 孙盼盼, 洪云, 虞乐, 李曙. 葛根素对人非小细胞肺癌A549细胞凋亡的作用[J]. 中国应用生理学杂志, 2017, 33(5): 466-469.
JIN Xin, SUN Pan-pan, HONG Yun, YU Le, LI Shu. Puerarin induces apoptosis in A549 cells. CJAP, 2017, 33(5): 466-469.
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