JIN Xin1, SUN Pan-pan2, HONG Yun3, YU Le4, LI Shu4
1. Department of Pharmacology, Wannan Medical College, Wuhu 241002, China;
2. Department of Nursing, Wannan Medical College, Wuhu 241002, China;
3. Department of Ultrasonography, Yijishan Hospital, Wuhu 241002, China;
4. Department of Pathophysiology, Wannan Medical College, Wuhu 241002, China
Abstract：Objective: To explore the effect of puerarin induced apoptosis in human non-small cell lung canner A549 cells and its possible mechanisms.Methods: A549 cells were cultivated in vitro. When 80%~90% confluence was reached, they were treated with puerarin in different concentration (60、120、240 μg/ml). The effects on cell apoptpsis were examined by CCK-8 colorimetry. Characteristic morphological changes of apoptosis were observed by AO/EB staining. The apoptosis of A549 cells were detected by flow cytometry analysis. The proteins level of Apelin and APJ were tested by Western blot.Results: CCK-8 test showed that puerarin could inhibit the proliferation of A549 cells in a time and concentration dependent manner. Flow cytometry analysis indicated that puerarin could make A549 cells apoptosis. Compared with the A549 cells group, the protein expression of Apelin/APJ was decreased in puerarin groups.Conclusion: Puerarin induces apoptosis in A549 cells, and its mechanism may through the regulation of Apelin/APJ expression.
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