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中国应用生理学杂志 ›› 2017, Vol. 33 ›› Issue (4): 351-356.doi: 10.12047/j.cjap.5537.2017.085

• 研究论文 • 上一篇    下一篇

间歇有氧运动对MI大鼠肾脏CD40表达的影响及可能机制

林琴琴1, 耿元文1, 高婧1, 田振军2   

  1. 1. 燕山大学 体育学院, 河北 秦皇岛 066004;
    2. 陕西师范大学 体育学院, 陕西 西安 710062
  • 收稿日期:2016-12-09 修回日期:2017-05-12 出版日期:2017-07-28 发布日期:2018-06-19
  • 通讯作者: 林琴琴,Tel:0335-8057020;E-mail:linqinqin@ysu.edu.cn E-mail:linqinqin@ysu.edu.cn
  • 基金资助:
    国家自然科学基金青年科学基金项目(31300978);河北省科技支撑计划项目(12277652);河北省高等学校科学技术研究项目(YQ2014004)

Effect of aerobic interval training on the expression of renal CD40 in a rat model with myocardial infarction and its mechanism

LIN Qin-qin1, GENG Yuan-wen1, GAO Jing1, TIAN Zhen-jun2   

  1. 1. Yanshan University, Qinhuangdao 066004;
    2. Shanxi Normal University, Xi'an 710062, China
  • Received:2016-12-09 Revised:2017-05-12 Online:2017-07-28 Published:2018-06-19
  • Supported by:
    国家自然科学基金青年科学基金项目(31300978);河北省科技支撑计划项目(12277652);河北省高等学校科学技术研究项目(YQ2014004)

摘要: 目的:探讨间歇有氧运动对心肌梗死(MI)大鼠肾脏CD40表达的影响,揭示运动改善MI肾脏功能的可能机制。方法:36只雄性SD大鼠,随机分为假手术组(Sham)、心肌梗死组(MI)、心梗+间歇运动组(ME),每组12只。MI组采用心脏左冠状动脉前降支(LAD)结扎法,建立MI模型。Sham组大鼠实施假手术,ME组大鼠在MI手术后1周进行8周跑台运动。运动开始速度为10 m/min运动10 min后,速度逐渐增至25 m/min×7 min,再以15 m/min×3 min运动,之后依次交替进行。每天60 min×1次,每周5 d,共8周。训练结束后次日,各组大鼠评定心功能,腹主动脉取血及获取肾脏组织后,测定肾脏胶原容积百分比(CVF)、CD40、hs-CRP、TNF-α、IL-6、p-NF-κBp65、BUN和sCr等指标变化。结果:与Sham组比较,MI组大鼠LVEDP升高,LVSP和±dp/dt max显著降低,肾脏CVF升高;MI后可见肾脏肾小管细胞胞浆中CD40阳性染色,CD40蛋白和mRNA表达增多,血清及肾脏hs-CRP、TNF-α和IL-6表达升高,同时肾脏p-NF-κBp65蛋白表达增多,血清BUN和sCr表达增高。与MI组比较,ME组大鼠LVEDP降低,LVSP和±dp/dt max升高,肾脏CVF降低;肾脏CD40蛋白和mRNA表达减少,血清及肾脏hs-CRP、TNF-α和IL-6表达降低;同时肾脏p-NF-κBp65蛋白表达降低,血清BUN和sCr表达减少。结论:间歇有氧运动可显著降低心梗大鼠肾脏CD40表达,抑制NF-κB通路,减少血清及肾脏炎症因子表达,改善心梗大鼠肾脏功能。

关键词: 心肌梗死, 间歇有氧运动, CD40, 肾脏, 炎症因子, 大鼠

Abstract: Objective: To study the effects of aerobic interval training (AIT) on renal cluster of differentiation 40 (CD40) expression in rats with myocardial infarction (MI) and its possible mechanism.Methods: Thirty-six rats were randomly divided into three groups (n=12):Sham, MI and MI with AIT (ME) groups. The MI model was established by ligation of the left anterior descending coronary artery. Treadmill training was performed five times a week for 8 weeks (AIT:60 min/day with 10 min of warm-up at 10 m/min and 50 min of exercise at 25 m/min 7 min interspersed with 3 min at 15 m/min). After training, cardiaorenal function and renal tissue remodeling were evaluated. The changes of CD40, high-sensitivity C reactive protein(hs-CRP), TNF-α, IL-6, p-NF-κBp65, blood urea nitrogen (BUN) and serum creatinine (sCr) were determined.Results: Compared with the sham group, MI significantly increased left ventricular end-diastolic pressure (LVEDP) and decreased left ventricular systolic pressure (LVSP) and left indoor pressure change rate peak (dp/dtmax) in the MI group, concomitant with the increase in renal collagen volume fraction (CVF), which was reversed by AIT in the ME group. Moreover, compared with the sham group, CD40 was largely dispersed within the cytoplasm of renal tubule cells in the MI group. Meanwhile, the expressions of renal CD40 mRNA and protein, the levels of serum and renal hs-CRP, TNF-α and IL-6, the phosphorylation of NF-κBp65 (p-NF-κBp65) and the levels of sCr and BUN were obviously increased in the MI group. Compared with the MI group, AIT decreased the expressions of renal CD40 mRNA and protein, the levels of serum and renal hs-CRP, TNF-α and IL-6 and the expression of p-NF-κBp65, as well as decreased the levels of sCr and BUN in the ME group.Conclusion: AIT reduces the expressions of renal CD40 protein and mRNA, inhibits NF-κB signaling pathway, and then decreases the levels of inflammatory factors thereby improve the renal dysfunction after MI.

Key words: myocardial infarction, aerobic interval training, CD40, kidney, inflammatory factor, rats

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