首页  期刊介绍 征稿简则 编委会 期刊征订 广告服务 留言板 联系我们 English

中国应用生理学杂志 ›› 2018, Vol. 34 ›› Issue (3): 263-267.doi: 10.12047/j.cjap.5540.2018.062

• 研究论文 • 上一篇    下一篇

马齿苋多糖对溃疡性结肠炎大鼠肠组织IL-6/STAT3及NF-κB的影响

范文涛, 王攀红, 王倩   

  1. 陕西中医药大学, 咸阳 712046
  • 收稿日期:2017-06-15 修回日期:2017-12-27 出版日期:2018-05-28 发布日期:2018-09-08
  • 通讯作者: 王倩,Tel:029-38185299;E-mail:674165202@qq.com E-mail:674165202@qq.com
  • 基金资助:
    国家自然科学基金(81202847,81470188,81673832);陕西省科技厅社发攻关项目(2016SF-375);陕西省中医药管理局项目(13-LC073)

Effects of purslane polysaccharide on the intestinal tissue IL-6/STAT3 and NF-kappa B of ulcerative colitis

FAN Wen-tao, WANG Pan-hong, WANG Qian   

  1. Shanxi University of Chinese Medicine, Xianyang 712046, China
  • Received:2017-06-15 Revised:2017-12-27 Online:2018-05-28 Published:2018-09-08
  • Supported by:
    国家自然科学基金(81202847,81470188,81673832);陕西省科技厅社发攻关项目(2016SF-375);陕西省中医药管理局项目(13-LC073)

摘要: 目的:采用TNBS (2,4,6-三硝基苯磺酸)复制溃疡性结肠炎大鼠模型,探索马齿苋多糖对溃疡性结肠炎大鼠肠组织IL6/STAT3及NF-κB的影响,明确IL-6/STAT3信号通路与慢性炎症性肠病发病的关系,为慢性溃疡性结肠炎的治疗寻找新靶点。方法:将40只SD大鼠随机分为对照组、模型组、美沙拉嗪组和马齿苋组(n=10)。采用TNBS诱导复制结肠炎模型,造模成功后第3天开始灌胃给药:美沙拉嗪组剂量为每次10 mg/kg,每日1次,连续3周;马齿苋组给予马齿苋多糖,每次10 ml/kg,每日1次,连续3周;模型组和对照组大鼠给予等体积生理盐水灌胃,每日1次,连续3周。收集大鼠结肠内容物称重,干燥后再次称重,取结肠组织作病理切片。采用ELISA试剂盒检测血清IL-6、IL-1β、TNF-α和核转录因子-kappa B (NF-κB)含量;免疫组化染色法测定结肠髓过氧化物酶(MPO);RT-PCR法检测信号转导和转录激活因子(STAT3)、IL-6的mRNA。结果:与模型组、美沙拉嗪组比较,马齿苋组大鼠排便状态明显改善,肠粘膜水肿减轻;血清IL-6、sIL-6Rα、gp130,肠组织MPO、NF-κB含量均降低(P<0.01)。与模型组比较,马齿苋组STAT3、IL-6mRNA的表达水平明显降低(P<0.01)。与对照组比较,上述指标无显著性差异(P>0.05)。结论:马齿苋多糖通过降低大鼠血清IL-6、sIL-6Rα、gp130含量及肠组织MPO、NF-κB水平,减轻sIL-6Rα与IL-6形成复合物所致的炎症反应;经IL-6/STAT3信号通路下调大鼠肠组织STAT3和IL-6的mRNA水平,从而抑制炎症的发生。

关键词: 马齿苋多糖, 溃疡性结肠炎, IL-6/STAT3, NF-κB、大鼠

Abstract: Objective: 2minus 2-trinitrobenzene sulfonic acid (TNBS) copy ulcerative colitis model of rats, to explore purslane polysaccharide on ulcerative colitis rats colon organization IL6/STAT3 and the influence of the NF-kappa B, clear IL-6/STAT3 signaling pathway and the relationship of chronic inflammatory bowel disease, looking for new targets for the treatment of chronic ulcerative colitis.Methods: Fourty rats were randomly divided into control group, model group, mesalazine group, and purslane group (n=10). The colonic contents were weighed and weighed again after drying, and the colonic tissues were biopsies. The contents of serum interleukin-6 (IL-6), interleukin 1 β (IL-1 β), tumor necrosis factor α (TNF-α), nuclear transcription factor kappa B (NF-kappa B) were detected by ELISA kit. Immunohistochemical staining was used to determine the colonic MPO (myeloperoxidase). RT-PCR method was used to detect signal transduction and transcriptional activator 3 (STAT3), IL-6 mRNA.Results: Compared with model group and mesalazine group, defecation status of rats in purslane group were markedly improved, intestinal mucosa edema reduced, the serum IL-6, intestinal tissue MPO, the NF-kappa B and serum sIL-6Rα, gp130 content were decreased (P<0.01). Compared with the model group, the expression levels of STAT3 and IL-6 mRNA in the purslane group were significantly reduced (P<0.01). There was no significant difference of testing indicator mentioned above between control group and purslane group (P>0.05).Conclusion: By lowering the serum IL-6, sIL-6Rα and gp130 in rats, the purslane polysaccharides reduced the levels of MPO and NF-kappa B in the intestinal tissues, and further reduced the inflammatory response caused by the formation of sIL-6R alpha and IL-6. By using IL-6/STAT3 signaling pathway, the mRNA expression levels of STAT3 and IL-6 of rats intestinal tissues were down-regulated to inhibit the occurrence of inflammation.

Key words: Purslane polysaccharide, inflammatory bowel disease, IL-6/STAT3 signaling pathway, rat

版权所有 © 2015 《中国应用生理学杂志》编辑部
京ICP备16058274号-1
地址:天津市和平区大理道1号,邮编:300050  电话:022-84655184  E-mail:tjzgyish@163.com
本系统由北京玛格泰克科技发展有限公司设计开发 技术支持:support@magtech.com.cn