细胞粘附分子CHL1缺失对炎症性肠病的影响

doi:10.12047/j.cjap.5567.2018.002

中国应用生理学杂志 ›› 2018, Vol. 34 ›› Issue (1): 4-7.doi: 10.12047/j.cjap.5567.2018.002

细胞粘附分子CHL1缺失对炎症性肠病的影响

王晓萌¹, 赵彤¹, 成祥¹, 郭宁¹, 朱玲玲¹, 施明², 吴奎武¹

1. 军事科学院军事医学研究院认知与脑科学研究所, 北京 100850;
2. 徐州医科大学肿瘤生物治疗研究所, 江苏徐州 221004

收稿日期:2017-05-22 修回日期:2017-10-18 出版日期:2018-01-28 发布日期:2018-06-19
通讯作者: 施明,Tel:13051909301,010-66932333;E-mail:shiming_amms@sina.cn,吴奎武,E-mail:ammswu@sina.com E-mail:shiming_amms@sina.cn;ammswu@sina.com
基金资助:
国家自然科学基金面上项目（81572845，81773086，81773258）

Effects of CHL1 deficiency,a cell adhesion molecule,on the inflammatory bowel disease

WANG Xiao-meng¹, ZHAO Tong¹, CHENG Xiang¹, GUO Ning¹, ZHU Ling-ling¹, SHI Ming², WU Kui-wu¹

1. Department of Brain Science and Stress, Institute of Cognition and Brain Sciences, Beijing 100850;
2. Jiangsu Province Cancer Biotherapy Institute, Xuzhou 221004, China

Received:2017-05-22 Revised:2017-10-18 Online:2018-01-28 Published:2018-06-19
Supported by:
国家自然科学基金面上项目（81572845，81773086，81773258）

摘要/Abstract

摘要： 目的：探讨细胞粘附分子CHL1缺失对炎症性肠病（IBD）的影响。方法：采用葡聚糖硫酸钠（DSS）建立CHL1（-/-）炎症性肠病小鼠模型，将10只C57/BL6为遗传背景的CHL1（+/+）型小鼠分为对照组、DSS模型组（n=5）；将10只C57/BL6为背景的CHL （-/-）型小鼠分为CHL1缺失组、CHL1缺失DSS模型组（n=5）。DSS模型组和CHL1缺失DSS模型组自主饮用7 d含1.5% DSS蒸馏水，随后改用蒸馏水饲喂2 d；对照组和CHL1缺失组连续饮用蒸馏水9 d，观察小鼠体重、血便、生存率及结肠组织长度的变化。结果：CHL1缺失DSS模型组小鼠在炎症性肠病的发生中便血明显，从应激的第7天开始，体重明显减轻，在第9天，CHL1缺失DSS模型组型小鼠死亡率明显增加；结肠长度明显缩短，损伤加重。结论：细胞粘附分子CHL1缺失加重炎症性肠病的发生。

关键词: CHL1, DSS, 炎症性肠病

Abstract: Objective: To investigate the effects of deficiency of CHL1 in inflammatory bowel disease (IBD).Methods: Dextran Sulfate Sodium (DSS)-induced colitis model was used to study the effects of deficiency of CHL1 on the development of IBD. Ten CHL1(+/+) mice in C57/BL6 background were randomly divided into CHL1(+/+) group and DSS-induced CHL1(+/+) group. Ten CHL1(-/-) mice in C57/BL6 background were randomly divided into CHL1(-/-) group and DSS-induced CHL1(-/-) group. DSS-induced CHL1(+/+) group and DSS-induced CHL1(-/-)group were fed with 1.5% DSS for 7 days, and then drinking distilled water for 2 days. CHL1(+/+) group and CHL1(-/-) group as control group were fed with distilled water for 9 days. The changes of weight, survival, fecal blood and the change of colon length in this study were observed.Results: On the 7^th day, the weight of DSS-induced CHL1(-/-) group were reduced significantly, and DSS-induced CHL1(-/-) group had extreme mortality on the 9th day. The fecal blood of DSS-induced CHL1(-/-) group also had higher score than that of DSS-induced CHL1(+/+) group. In the DSS-induced CHL1(-/-) group,the length of colon was shortened obviously.Conclusion: The loss of CHL1 aggravates the development of IBD.

Key words: CHL1, DSS, IBD

王晓萌, 赵彤, 成祥, 郭宁, 朱玲玲, 施明, 吴奎武. 细胞粘附分子CHL1缺失对炎症性肠病的影响[J]. 中国应用生理学杂志, 2018, 34(1): 4-7.

WANG Xiao-meng, ZHAO Tong, CHENG Xiang, GUO Ning, ZHU Ling-ling, SHI Ming, WU Kui-wu. Effects of CHL1 deficiency,a cell adhesion molecule,on the inflammatory bowel disease[J]. CJAP, 2018, 34(1): 4-7.

参考文献

[1] Mikocka-Walus AA, Turnbull DA, Moulding NT, et al. Controversies surrounding the comorbidity of depression and anxiety in inflammatory bowel disease patients:a literature review[J]. Inflamm Bowel Dis, 2007, 13(2):225-234.
[2] Ng WK, Wong SH, Ng SC. Changing epidemiological trends of inflammatory bowel disease in Asia[J]. Intest Res, 2016, 14(2):111-119.
[3] Kaplan GG. The global burden of IBD:from 2015 to 2025[J]. Nat Rev Gastroenterol Hepatol, 2015, 12(12):720-727.
[4] Ahmad R, Sorrell MF, Batra SK, et al. Gut permeability and mucosal inflammation:bad, good or context dependent[J]. Mucosal Immunol, 2017, 10(2):307-317.
[5] Cromer WE, Mathis JM, Granger DN, et al. Role of the endothelium in inflammatory bowel diseases[J]. World J Gastroenterol, 2011, 17(5):578-593.
[6] Nieves JR, Gorfu G, Ley K. Leukocyte adhesion molecules in animal models of inflammatory bowel diseases[J]. Inflamm Bowel Dis, 2008, 14(12):1715-1735.
[7] Rolf B, Lang D, Hillenbrand R, et al. Altered expression of CHL1 by glial cells in response to optic nerve injury and intravitreal application of fibroblast growth factor-2[J]. J Neurosci Res, 2003, 71(6):835-843.
[8] Nishimune H, Bernreuther C, Carroll P, et al. Neural adhesion molecules L1 and CHL1 are survival factors for motoneurons[J]. J Neurosci Res, 2005, 80(5):593-599.
[9] Holm J, Hillenbrand R, Steuber V, et al. Structural features of a close homologue of L1(CHL1) in the mouse:a new member of the L1 famliy of neural recognition molecules[J]. Eur J Neurosci, 1996, 8(8):1613-1629.
[10] Fearon ER, Cho KR, Nigro JM, et al. Identification of a chromosome 18q gene that is altered in colorectal cancers[J]. Science, 1990, 247(4938):49-56.
[11] Jakovcevski I, Wu J, Karl N, et al. Glial scar expression of CHL1, the close homolog of the adhesion molecule L1, limits recovery after spinal cord injury[J]. J Neurosci, 2007, 27(27):7222-7233.
[12] Schmalbach B, Lepsveridze E, Djogo N, et al. Age-dependent loss of parvalbumin-expressing hippocampal interneurons in mice deficient in CHL1, a mental retardation and schizophrenia susceptibility gene[J]. J Neurochem, 2015, 135(4):830-844.
[13] Huang X, Zhu LL, Zhao T, et al. CHL1 negatively regulates the proliferation and neuronal differentiation of neural progenitor cells through activation of the ERK1/2 MAPK pathway[J]. Mol Cell Neurosci, 2011, 46(1):296-307.
[14] Senchenko VN, Krasnov GS, Dmitriev AA, et al. Differential expression of CHL1 gene during development of major human cancers[J]. PLoS One, 2011, 6(3):15612.
[15] Bonaz BL, Bernstein CN. Brain-gut interactions in inflammatory bowel disease[J]. Gastroenterol, 2013, 144(1):36-49.
[16] Deng Q, Chen H, Liu Y, et al. Psychological stress promotes neutrophil infiltration in colon tissue through adrenergic signaling in DSS-induced colitis model[J]. Brain Behav Immun, 2016, 57:243-254.
[17] 于正, 刘红菊, 顿桓桓, 等. 白头翁汤对炎症性肠病中促炎细胞因子表达的影响[J]. 中国应用生理学杂志, 2011, 27(4):416-419.
[18] 黄循铷, 王承党, 王瑞幸, 等. 溃疡性结肠炎小鼠肠道通透性改变与TNF-α及NF-κB P65的关系[J]. 中国应用生理学杂志, 2016, 32(2):112-115.

细胞粘附分子CHL1缺失对炎症性肠病的影响

Effects of CHL1 deficiency,a cell adhesion molecule,on the inflammatory bowel disease

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

编辑推荐

Metrics

本文评价