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中国应用生理学杂志 ›› 2017, Vol. 33 ›› Issue (4): 373-376.doi: 10.12047/j.cjap.5569.2017.090

• 研究论文 • 上一篇    下一篇

TNF-α/NF-κB信号通路在依那西普缓解大鼠类风湿性关节炎中的作用

刘彩丽, 王颖瑛   

  1. 安阳市妇幼保健院检验科, 河南 安阳 455000
  • 收稿日期:2017-02-23 修回日期:2017-06-05 出版日期:2017-07-28 发布日期:2018-06-19
  • 通讯作者: 王颖瑛,Tel:13569067892;E-mail:10485536@qq.com E-mail:10485536@qq.com
  • 基金资助:
    王颖瑛,Tel:13569067892;E-mail:10485536@qq.com

Effects of TNF-alpha/NF-kappa B signaling pathway on etanercept alleviating rheumatoid arthritis

LIU Cai-li, WANG Ying-ying   

  1. Department of Clinical Laboratory the Maternal and Child Health Hospital of Anyang City, Anyang 455000, China
  • Received:2017-02-23 Revised:2017-06-05 Online:2017-07-28 Published:2018-06-19
  • Contact: 王颖瑛,Tel:13569067892;E-mail:10485536@qq.com E-mail:10485536@qq.com
  • Supported by:
    王颖瑛,Tel:13569067892;E-mail:10485536@qq.com

摘要: 目的:探讨依那西普(ETN)对胶原诱导大鼠类风湿性关节炎(CIA)的缓解作用及其机制。方法:实验动物分为3组(n=10):①对照组;②生理盐水治疗组(NS组);③(0.5 mg/kg)依那西普治疗组(ETN组)。其中②和③组的鼠在炎症分值达到两分及以上时随机分组开始治疗。药物采用皮下注射牛Ⅱ型胶原诱导Wistar大鼠建立类风湿性关节炎模型。治疗前和治疗期间每周测量足趾容积和体重,治疗4周后处死,ELISA检测血清TNFα、Ang-1、IL-1β、IL-6、IL-8水平;取足趾部位肌肉提取胞浆和胞核蛋白,Western blot检测NF-κB含量及其亚细胞定位。结果:依那西普治疗组较之生理盐水治疗组能显著缓解类风湿性关节炎症症状、降低TNFα以及下游炎症因子水平(P<0.05);能抑制炎症状况下NF-κB的核内定位增加、胞浆定位减少的状况。结论:依那西普可通过抑制TNFα/NF-κB信号通路缓解大鼠类风湿性关节炎。

关键词: 依那西普, 类风湿性关节炎, TNFα/NF-κB信号通路, 大鼠

Abstract: Objective: To investigate the effects of etanercept on collagen induced arthritis rats.Methods: The rheumatoid arthritis model was established via subcutaneous injection of bovine type Ⅱ collagen, and the effects of etanercept was compared through three groups:①the normal control;②arthritis model treated with saline;③arthritis model treated with etanercept (0.5 mg/kg, intraperitoneal injection) with 10 rats in each group;group②and group③ were selected by arthritis index (≥ 2)and grouped randomly. The body weight and toe volume were measured at just before and during treatment weekly. Rats were sacrificed after 4 weeks of treatment, and the serum levels of tumor necrosis factor α(TNF-α), angiopoietin-1(Ang-1), IL-1β, IL-6 and IL-8 were tested by ELISA. Nuclear factor-kappa B(NF-κB) levels and subcellular locations in toe muscles were test by Western blot.Results: Etanercept significantly relieved inflammation of rheumatoid arthritis, lowered the TNF-α and relative inflammatory factors levels compared with saline treatment (P<0.05). The results of Western blot showed that etanercept significantly suppressed the nuclear location of NF-κB p105/p50 caused by inflammation.Conclusion: The anti-inflammatation effect of etanercept may be related to its activity in inhibiting the activation of TNF-α/NF-κB pathway.

Key words: etanercept, rheumatoid arthritis, TNF-α/NF-κB pathway, rats

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