玛咖提取物对运动耐力和脊神经元线粒体超微结构的影响

doi:10.12047/j.cjap.5601.2017.127

中国应用生理学杂志 ›› 2017, Vol. 33 ›› Issue (6): 535-538.doi: 10.12047/j.cjap.5601.2017.127

玛咖提取物对运动耐力和脊神经元线粒体超微结构的影响

俞发荣¹, 杨博¹, 李作平², 连秀珍¹, 谢明仁¹, 李登楼¹, 张诗爽¹

1. 甘肃政法学院甘肃省证据科学技术研究与应用重点实验室, 兰州 730070;
2. 甘肃政法学院体育教学部, 兰州 730070

收稿日期:2016-07-28 修回日期:2017-05-15 出版日期:2017-11-28 发布日期:2018-06-19
基金资助:
甘肃省基础研究创新群体项目（145RJIA333）；兰州市科技计划项目（2014-1-182；2015-3-80）；甘肃省高校科技创新团队项目（2016C-09）；兰州市人才创新创业项目（2016-RC-85）；西北民族地区侦查理论与实务研究中心项目；甘肃政法学院重大项目（2016XZD15）；甘肃政法学院"文翰学者"项目

Effects of the maca extract on the ultrastructures of mitochondria in the spinal nerve cell and exercise endurance

YU Fa-rong¹, YANG Bo¹, LI Zuo-ping², LIAN Xiu-zhen¹, XIE Ming-ren¹, LI Deng-lou¹, ZHANG Shi-shuang¹

1. Key Laboratory of Evidence of Science and Technology Research and Application, Gansu Institute of Political Science and Law, Lanzhou 730070, China;
2. Department of Physical Education, Gansu Institute of Political Science and Law, Lanzhou 730070, China

Received:2016-07-28 Revised:2017-05-15 Online:2017-11-28 Published:2018-06-19
Contact: 李作平,Tel:18189651683;E-mail:tim9898@163.com E-mail:tim9898@163.com
Supported by:
甘肃省基础研究创新群体项目（145RJIA333）；兰州市科技计划项目（2014-1-182；2015-3-80）；甘肃省高校科技创新团队项目（2016C-09）；兰州市人才创新创业项目（2016-RC-85）；西北民族地区侦查理论与实务研究中心项目；甘肃政法学院重大项目（2016XZD15）；甘肃政法学院"文翰学者"项目

摘要/Abstract

摘要： 目的：探索玛咖提取物对运动耐力和脊神经元线粒体超微结构的影响。方法：50只Wistar大鼠随机分为5组。对照组：不进行游泳，灌胃等量蒸馏水；单纯游泳组：游泳，灌胃等量蒸馏水，玛咖提取物组（设4.0，5.3，8.0 g/kg 3个剂量组）：游泳，给予相应剂量的玛咖提取物。游泳大鼠在游泳池内循环水流自由游泳，连续游泳并给药15 d，第16天游泳耐力测试后无痛处死大鼠，用投射电子显微镜观察脊髓神经元线粒体超微结构，放免法测定肌肉肌糖元、检测肌肉中丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和游离钙水平。结果：与单纯游泳组比，沉下前游泳时间和游泳总时间分别延长了（%）19.83、60.28、77.55和55.34、73.91、94.47，差异均有统计学意义（P均<0.01）；沉下次数分别减少了（%）34.35、51.18、57.96，差异有统计学意义（P<0.01）；MDA和游离钙含量分别降低了（%）20.10、31.49、38.72，差异有统计学意义（P<0.01）和6.42、17.58、26.35；SOD、GSH-Px和肌糖原含量分别升高了（%）5.12、22.74、52.53、44.22、77.79、98.45，差异有统计学意义（P<0.01）和35.08、47.83、81.88，差异有统计学意义（P<0.01）；脊神经元线粒体的体密度（VD）、面密度（SD）和数密度（ND）分别减小了（%）7.79、18.18、31.17，16.95、27.34、43.31和13.51、23.19、43.15。结论：玛咖提取物具有保护脊髓神经元线粒体结构、抗氧化、增加肌糖原和提高运动能力等作用。

关键词: 玛咖提取物, 运动耐力, 线粒体, Wistar大鼠

Abstract: Objective: To investigate the effects of maca extract on the ultrastructures of mitochondria in the spinal nerve cell and exercise endurance.Methods: The Wistar rats were randomly divided into 5 groups, including the control group (no swimming), the swimming group (free swimming), and 3 treatment groups treated with the maca extract at the doses of 4.0, 5.3 and 8.0 g/kg body weight. The animals in swimming and treatment groups were then for free swimming in the circulating water flow daily for 15 days. On the 16^th day after swimming endurance, the spinal and muscular tissues were collected from all groups. The mitochondrial ultrastructures of the neurons of the spinal cells were observed with the projection electron microscope, and the levels of the glycogen, malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and Ca²⁺ in muscle tissues were determined by the RIA method.Results: When rats were treated with maca extract (at 4.0, 5.3, 8.0 g/kg body weight), the total swimming time and the swimming duration before sinking were increased by 19.83%, 60.28%, 77.55%, and 55.34%, 73.91%, 94.47%, respectively, compared with the simple swimming group(P<0.01), while the sinking times were decreased by 34.35%, 51.18% and 57.96%, compared with those of the swimming group. Also, the levels of SOD, GSH-Px, and muscle glycogen in three treatment groups were enhanced by 5.12%, 22.74%, 52.53%, 44.22%, 77.79%, 98.45%(P<0.01), and 35.08%, 47.83%,81.88% (P<0.01)respectively over the swimming rats without treatment, but the MDA content and the Ca²⁺ levels were reduced by 20.10%, 31.49% 38.72%, and 6.42%, 17.58%, 26.35%,compared with the simple swimming group(P<0.01). In addition, compared to the swimming group, the mitochondrial densities of volume (VD), surface (SD) and numbers (ND) of spinal nerve cells in rats treated with maca extract (4.0, 5.3, 8.0 g/kg body weight) were reduced by 7.79%, 18.18%, 31.17%, 16.95%, 27.34%, 43.31% and 13.51%, 23.19%, 43.15%, respectively.Conclusion: Our results demonstrated the protective effects of maca extract on the mitochondria of spinal cell and suggested that maca extract could improve the muscle antioxidant activity by increasing the levels of SOD, GSH-Px, and muscle glycogen.

Key words: maca extract, movement endurance, mitochondria, Wistar rats

俞发荣, 杨博, 李作平, 连秀珍, 谢明仁, 李登楼, 张诗爽. 玛咖提取物对运动耐力和脊神经元线粒体超微结构的影响[J]. 中国应用生理学杂志, 2017, 33(6): 535-538.

YU Fa-rong, YANG Bo, LI Zuo-ping, LIAN Xiu-zhen, XIE Ming-ren, LI Deng-lou, ZHANG Shi-shuang. Effects of the maca extract on the ultrastructures of mitochondria in the spinal nerve cell and exercise endurance[J]. CJAP, 2017, 33(6): 535-538.

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玛咖提取物对运动耐力和脊神经元线粒体超微结构的影响

Effects of the maca extract on the ultrastructures of mitochondria in the spinal nerve cell and exercise endurance

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