6周大强度训练对大鼠肾功能的影响及其机制

doi:10.12047/j.cjap.5641.2018.017

中国应用生理学杂志 ›› 2018, Vol. 34 ›› Issue (1): 65-68.doi: 10.12047/j.cjap.5641.2018.017

6周大强度训练对大鼠肾功能的影响及其机制

牛衍龙¹, 曹建民¹, 周海涛², 李浩²

1. 北京体育大学, 北京 100084;
2. 北京联合大学, 北京 100023

收稿日期:2017-07-10 修回日期:2017-11-27 出版日期:2018-01-28 发布日期:2018-06-19
通讯作者: 周海涛,Tel:13611383040;E-mail:zsettle@sina.com E-mail:zsettle@sina.com
基金资助:
运动应激适应国家体育总局重点实验室研究课题（2016SYS005）；北京市高等学校高水平人才交叉培养-实培计划项目；北京市朝阳区协同创新项目（CYXC1508）

Effects and mechanisms of 6-week intensive training on renal function in rats

NIU Yan-long¹, CAO Jian-min¹, ZHOU Hai-tao², LI Jie²

1. Beijing Sport University, Beijing 100084;
2. Beijing Union University, Beijing 100023, China

Received:2017-07-10 Revised:2017-11-27 Online:2018-01-28 Published:2018-06-19
Supported by:
运动应激适应国家体育总局重点实验室研究课题（2016SYS005）；北京市高等学校高水平人才交叉培养-实培计划项目；北京市朝阳区协同创新项目（CYXC1508）

摘要/Abstract

摘要： 目的：研究6周大强度训练对大鼠肾功能影响及运动性蛋白尿的机制。方法：6周龄SD雄性大鼠36只随机分为：安静对照组（C组，n=12）和大强度训练组（M组，n=24）。大鼠适应性饲养4 d后，C组不进行任何运动，M组采用6周递增负荷游泳训练。每周训练6 d，每天1次。第4周起开始负重（体重的1%）并逐渐递增（体重的6%）。各组大鼠末次训练结束后30 min取单次尿，24 h后腹主静脉取血，取双侧肾脏待测。HE染色观察肾脏肾小球结构，酶联免疫吸附法测定尿总蛋白、尿微量白蛋白、中性粒细胞明胶酶相关脂质运载蛋白，碱性苦味酸法测试尿肌酐，比色法测定血清肌酐、尿素氮，蛋白质免疫印迹法检测肾组织Nephrin蛋白表达量，放射免疫法测试血清睾酮、皮质酮和肾组织及血液中肾素-血管紧张素系统相关指标变化。结果：与C组比较，M组血清睾酮/皮质酮显著降低（P<0.01）；尿液蛋白总量、微量白蛋白、微量白蛋白与肌酐比值、中性粒细胞明胶酶相关脂质运载蛋白、血清尿素氮、肌酐均显著升高（P<0.01）；肾小球结构明显改变，且Paller评分明显增高（P<0.01）；肾组织Nephrin蛋白表达量明显降低（P<0.01）；肾脏内部与循环血液中肾素活性、血管紧张素Ⅱ显著增高（P<0.01）。结论：6周大强度训练对大鼠肾功能影响及运动性蛋白尿的机制可能为过度训练诱导肾脏内部及循环系统中肾素-血管紧张素系统持续兴奋，并下调Nephrin蛋白的表达，进而导致肾脏结构与功能异常，出现蛋白尿。

关键词: 长期大强度训练, 大鼠, 裂孔隔膜, Nephrin, 肾素-血管紧张素系统

Abstract: Objective: To study the effect of 6-week intensive training on renal function in rats and the mechanism of exercise-induced proteinuria.Methods: Thirty-six male SD rats, aged 6 weeks, were divided into two groups, including a control group(C,n=12)and an overtraining group(M,n=24). After the rats adapted to feeding for 4 d, group C did not carry out any exercise, and the M group did 6-week of increasing load swimming, 6 days a week, once a day. Started with the load of 1%weight at the beginning of the 4^th week,and gradually increased (to 6% weight). Took a single urine from both groups 30 min after the end of the training. Blood was taken from the main ventral vein, and the bilateral kidneys were to be tested. The levels of tested urine protein, microalbumin and neutrophil gelatinase associated lipocalin(NGAL) was determined by using enzyme linked immunosorbent assaytest. The content of urine creatinine was tested with alkaline picric acid method,. The serum levels of colorimetric method to determine serum creatinine and urea nitrogen were determined by colorimetric method. The expression of Nephrin in renal tissue was detected by Western blot and the radioimmunoassay was used to test serum testosterone, corticosterone and renin-angiotensin system related index.Results: Compared with group C, the serum testosterone/cortisone(T/C) of group M was decreased significantly (P<0.01). The urine total protein(TP), microalbumin (mAlb), microalbumin/creatinine (mAlb/CRE), NGAL, blood urea nitrogen (BUN) and serum creatinine(SCr) were increased significantly (P<0.01). The abnormality of glomerular structure was obvious, and the paller scores were higher. The protein expression of Nephrin was obviously down decreased (P<0.01). The renin activity (Ra) and angiotension Ⅱ (Ang Ⅱ) in renal and circulating blood were decreased significantly (P<0.01).Conclusion: The effects of 6-week intensive training on renal function in rats and the mechanism of exercise-induced proteinuria may be that overtraining can induce the continuous excitation of Reninrenin activity in renal and circulating blood, down-regulated the expression of Nephrin, lead to abnormality of renal structure and function, and proteinuria.

Key words: long-term strength training, rat, slit diaphragm, nephrin, renin angiotensin system

牛衍龙, 曹建民, 周海涛, 李浩. 6周大强度训练对大鼠肾功能的影响及其机制[J]. 中国应用生理学杂志, 2018, 34(1): 65-68.

NIU Yan-long, CAO Jian-min, ZHOU Hai-tao, LI Jie. Effects and mechanisms of 6-week intensive training on renal function in rats[J]. CJAP, 2018, 34(1): 65-68.

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6周大强度训练对大鼠肾功能的影响及其机制

Effects and mechanisms of 6-week intensive training on renal function in rats

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