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中国应用生理学杂志 ›› 2018, Vol. 34 ›› Issue (4): 375-378.doi: 10.12047/j.cjap.5657.2018.086

• 研究论文 • 上一篇    下一篇

苦参碱对大鼠乳腺癌细胞荷瘤生长及其炎性因子与免疫功能的影响

张瑞奎, 汪超   

  1. 武警后勤学院附属医院肿瘤外科, 天津 300162
  • 收稿日期:2017-12-08 修回日期:2018-01-18 出版日期:2018-07-28 发布日期:2019-02-21
  • 通讯作者: 汪超 E-mail:tjwjzrk@126.com
  • 基金资助:
    武警后勤学院种子基金(WHB201214)

Effect of matrine on tumor growth and inflammatory factors and immune function in Wistar rat with breast cancer

ZHANG Rui-kui, WANG Chao   

  1. Department of Surgical Oncology, Affiliated Hospital of Logistics College of Chinese People's Armed Police Force, Tianjin 300162, China
  • Received:2017-12-08 Revised:2018-01-18 Online:2018-07-28 Published:2019-02-21
  • Supported by:
    武警后勤学院种子基金(WHB201214)

摘要: 目的:探讨苦参碱对大鼠乳腺癌细胞荷瘤大鼠生长及其炎性因子与免疫功能的影响。方法:雌性Wistar大鼠60只,随机分为对照组(n=10),乳腺癌细胞荷瘤大鼠建模组(n=50)。建模大鼠再随机分为5组(n=10):模型组、苦参碱低(50 mg/kg)、中(100 mg/kg)、高(200 mg/kg)剂量组和香菇多糖(200 mg/kg)组。除对照组外,每只大鼠右腋皮下接种0.4 ml Walker 256乳腺癌细胞悬液(5×107 cells/ml)。按10 ml/kg的体积腹腔注射给药,2次/日,连续14 d。14 d后麻醉大鼠,腹主动脉取血,取瘤组织称取质量,计算抑瘤率;检测大鼠外周血IL-2、IFN-γ、IL-6、IL-10、TGF-β、CD3+、CD4+、CD8+、IgG、IgM、IgA水平。结果:苦参碱低、中、高剂量组和香菇多糖组大鼠瘤块平均质量分别为(4.99±0.93)g、(4.52±0.92)g、(4.22±1.18)g、(4.52±0.92)g,与模型组(6.62±1.20)g比较均明显降低(P<0.01)。苦参碱各剂量组与香菇多糖组比较,瘤块平均质量无明显差异(P>0.05)。苦参碱低、中、高剂量组和香菇多糖组抑瘤率分别为24.6%、31.7%、36.3%和27.9%,组间无统计学差异。模型组大鼠IL-2、IFN-γ、CD8+较对照组明显降低(P<0.01);而IL-6、IL-10、TGF-β、CD3+、CD4+、IgG、IgM、IgA较对照组明显升高(P<0.01)。苦参碱低、中、高剂量组和香菇多糖组IL-2、IFN-γ、CD8+较模型组显著升高(P<0.01,P<0.05);而IL-6、IL-10、TGF-β、CD3+、CD4+、IgG、IgM、IgA较模型组明显降低(P<0.01,P<0.05)。与香菇多糖组比较,苦参碱低、中剂量组IgM、IgA明显升高(P<0.01,P<0.05);高剂量组IL-2和IFN-γ、IgA水平明显升高(P<0.01,P<0.05);低剂量组IFN-γ水平明显降低(P<0.05);高剂量组IL-10、CD4+水平明显降低(P<0.01,P<0.05)。结论:苦参碱具有明显的抑制肿瘤生长的作用,其抑瘤机制与其提高细胞和体液免疫、减轻炎症反应有关。

关键词: 苦参碱,乳腺癌细胞荷瘤, 大鼠, 炎性因子, 免疫功能

Abstract: Objective: To study the effect of matrine on tumor growth, inflammatory factors and immune function in Wistar rat with breast cancer. Methods: Sixty female Wistar rats were randomly divided into control group (n=10) and the modeling group of breast cancer cell tumor-bearing rat (n=50), then the rats in modeling group were randomly divided into five groups (n=10):vehicle group, matrine low dose group (50 mg/kg), medium dose group (100 mg/kg), high dose group (200 mg/kg), and lentinan group (200 mg/kg). Except the control group, each rat in the other groups was subcutaneously inoculated 0.4 ml Walker 256 breast cancer cell suspension (5×107 cells/ml) in the right axillary. Each group was treated with corresponding drug by ig administration (10 ml/kg body weight) twice a day for 14 days. After 14 days, the blood sample was collected from ventral aorta, the tumor was removed and weighed to calculate tumor inhibitory rate. The levels of interleukin-2 (IL-2), interferon-γ (IFN-γ), interleukin-6 (IL-6), interleukin-10 (IL-10), transforming growth factor-β (TGF-β), CD3+, CD4+, CD8+, IgG, IgM, IgA in peripheral blood were determined. Results: The mean tumor weight of matrine low-dose, medium-dose, high-dose groups and lentinan group were (4.99±0.93) g, (4.52±0.92) g, (4.22±1.18) g and (4.52±0.92) g respectively, which were significantly lower than that in model group. There was no statistical difference on the mean tumor weight among matrine groups and lentinan group (P>0.05). After the drug intervention, the tumor inhibitory rates of matrine low-dose, medium dose, high-dose groups and lentinan group were 24.6%, 31.7%, 36.3%, and 27.9% respectively, there was no statistical difference among the four groups. The levels of IL-2, IFN-γ, CD8+ in vehicle group were lower than those of control group obviously (P<0.01), however, the levels of IL-6, IL-10, TGF-β, CD3+, CD4+, IgG, IgM, IgA were higher significantly than those of control group (P<0.01). The levels of IL-2, IFN-γ, CD8+ in matrine low-dose, medium dose, high-dose groups and lentinan group were higher than those of vehicle group obviously (P<0.01, P<0.05); while the levels of IL-6, IL-10, TGF-β, CD3+, CD4+, IgG, IgM, IgA were lower than those of model group markedly (P<0.01, P<0.05). The levels of IgM and IgA in matrine low-dose and medium-dose groups were higher than those of lentinan group obviously (P<0.01, P<0.05); the levels of IL-2, IFN-γ and IgA in matrine high-dose group were higher than those of lentinan group obviously (P<0.01, P<0.05); while the levels of IFN-γ in matrine low-dose group were lower than those of lentinan group markedly (P<0.05); the levels of IL-10 and CD4+ in matrine high-dose group were lower than those of lentinan group markedly (P<0.01, P<0.05). Conclusion: Matrine has an obvious antitumor action which is related to its ability to enhance cellular and humoral immunity, reduce inflammatory reaction.

Key words: matrine, breast cancer cell tumor-bearing, rats, inflammatory factor, immune function

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