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中国应用生理学杂志 ›› 2018, Vol. 34 ›› Issue (6): 485-489.doi: 10.12047/j.cjap.5662.2018.108

• 研究论文 • 上一篇    下一篇

氨基羟乙酸对慢性酒精中毒大鼠学习记忆的影响及可能的机制

秦浩志1, 姜洪波1,3, 代玄1, 黄亚迪1, 罗晓秋1, 张瑞岭1,2, 杜爱林1,2   

  1. 1. 新乡医学院生理学与神经生物学教研室, 中英脑功能损伤联合实验室, 河南省高校脑研究重点实验室培育基地, 河南 新乡 453003;
    2. 新乡医学院第二附属医院 河南省生物精神病学重点实验室, 河南 新乡 453003;
    3. 新乡医学院第三附属医院, 河南 新乡 453003
  • 收稿日期:2017-11-29 修回日期:2018-11-05 出版日期:2018-11-28 发布日期:2019-03-09
  • 通讯作者: 张瑞岭, 杜爱林 E-mail:duailin@126.com;zhangruilingxx@163.com
  • 基金资助:
    国家自然科学基金(81471351);河南省教育厅课题(15A310006);河南省卫生科技创新型人才工程专项经费(3052);河南省高校科技创新团队(17IRTSTHN023)

Effects of aminooxyacetic acid on the learning and memory ability and its possible mechanism in rats with chronic alcoholism

QIN Hao-zhi1, JIANG Hong-bo1,3, DAI Xuan1, HUANG Ya-di1, LUO Xiao-qiu1, ZHANG Rui-ling1,2, DU Ai-lin1,2   

  1. 1. Department of Physiology and Neurobiology and Sino-UK Joint Laboratory for Brain Function and Injury, Henan Provincial Key Laboratory of Brain Research, Xinxiang Medical University, Xinxiang 453003;
    2. The Second Affiliated Hospital of Xinxiang Medical University, Key Laboratory of Biological Psychiatry in Henan Province, Xinxiang 453003;
    3. The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang 453003, China
  • Received:2017-11-29 Revised:2018-11-05 Online:2018-11-28 Published:2019-03-09
  • Supported by:
    国家自然科学基金(81471351);河南省教育厅课题(15A310006);河南省卫生科技创新型人才工程专项经费(3052);河南省高校科技创新团队(17IRTSTHN023)

摘要: 目的:探究氨基羟乙酸(AOAA)对慢性酒精中毒大鼠学习记忆能力及可能机制的影响。方法:将60只SD雄性大鼠随机均分为3组(n=20):空白对照组、模型组和治疗组。模型组和治疗组饮含6%(v/v)酒精水溶液28 d。14 d后,治疗组连续14 d腹腔注射AOAA(5 mg/kg·d)注射液,其余两组注射等量生理盐水。实验结束前5 d连续进行5 d的水迷宫实验,实验结束取大鼠海马组织检测H2S含量、线粒体ATP酶活性及5-HT受体蛋白的表达。结果:与空白对照组比较,模型组大鼠水迷宫实验的第2~4日潜伏期、第2~4日游泳距离、H2S含量均升高,ATP酶活性和海马CA1、CA3区5-HT受体阳性表达明显下降(P<0.01);与模型组比较,治疗组大鼠第2~4日潜伏期、第2~4日游泳距离、H2S含量均下降,ATP酶活性和海马CA1、CA3区5-HT受体阳性表达显著升高(P< 0.01)。结论:AOAA能够减轻慢性酒精中毒大鼠的症状,可能与AOAA影响H2S的含量、线粒体酶活性、5-HT受体的含量有关。

关键词: 氨基羟乙酸, 慢性酒精中毒, 大鼠, 海马, 学习记忆能力

Abstract: Objective: To investigate the effects of aminooxyacetic acid (AOAA) on learning and memory ability and possible mechanisms in rats with chronic alcoholism.Methods: Sixty SD male rats were randomly divided into three groups on average.The model group rats and the remedy group rats were fed with the water containing (v/v) 6% alcohol for 28 days.After 14 days, the remedy group rats were treated with AOAA (5 mg/kg·d) by intraperitoneal injection once a day for 14 days and the other two group rats were treated with the equal amount of saline by intraperitoneal injection every day.Five days before the end of the experiment, the water maze test was carried out to test the learning and memory ability of rats for 5 days.Subsequently, the content of H2S, the activity of ATP enzyme and the expression of 5-HT in hippocampus were measured.Results: Compared with the rats in the control group, the latency and the swimming distance of the 2nd to the 4th day, the content of H2S in hippocampus of rats in the model group were all increased, the mitochondrial ATP enzyme activity in hippocampus and the positive expression of 5-HT in hippocampus CA1 and CA3 of rats in the model group were decreased (P<0.01).Compared with the rats in the model group, the latency and the swimming distance of the 2nd to the 4th day, the content of H2S in hippocampus of the rats in the remedy group were decreased, the mitochondrial ATP enzyme activity in hippocampus and the positive expression of 5-HT in hippocampus CA1 and CA3 of rats in the model group were increased (P<0.01).Conclusion: AOAA could alleviate the symptoms of chronic alcoholism rats, which may be related to the effects of AOAA on the content of H2S, the mitochondrial enzyme activity and the expression of 5-HT in hippocampus.

Key words: aminooxyacetic acid, chronic alcoholism, rats, 5-HT, mitochondria, learning and memory abilities

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