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中国应用生理学杂志 ›› 2018, Vol. 34 ›› Issue (3): 268-272.doi: 10.12047/j.cjap.5663.2018.063

• 研究论文 • 上一篇    下一篇

白扁豆多糖对人胃癌细胞凋亡的作用及其机制

张艳姿1,2, 柯瑞君2, 蒋盼若2, 杨林军3, 陈佳玉1,4   

  1. 1. 温州医科大学检验学院、生命科学学院, 浙江温州 325035;
    2. 绍兴文理学院医学院, 浙江绍兴 312000;
    3. 台州市立医院, 浙江台州 318000;
    4. 台州学院医学院, 浙江台州 318000
  • 收稿日期:2017-12-28 修回日期:2018-02-23 出版日期:2018-05-28 发布日期:2018-09-08
  • 通讯作者: 陈佳玉,Tel:0575-88345826;E-mail:chenyujia10@163.com E-mail:chenyujia10@163.com
  • 基金资助:
    国家自然科学基金资助项目(81402979);浙江省科技厅资助项目(2015C33270);浙江省卫生厅资助项目(2014KYA230)

Effect of white lentil polysaccharides on the apoptosis of human gastric cancer cells and its mechanism

ZHANG Yan-zi1,2, KE Rui-jun2, JIANG Pan-ruo2, YANG Lin-jun3, CHEN Jia-yu1,4   

  1. 1. School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325035;
    2. Medical School, Shaoxing University, Shaoxing 312000;
    3. Taizhou Municipal Hospital, Taizhou 318000;
    4. Taizhou University Medical School, Taizhou 318000, China
  • Received:2017-12-28 Revised:2018-02-23 Online:2018-05-28 Published:2018-09-08
  • Supported by:
    国家自然科学基金资助项目(81402979);浙江省科技厅资助项目(2015C33270);浙江省卫生厅资助项目(2014KYA230)

摘要: 目的:探讨白扁豆多糖对人胃癌细胞凋亡的作用及其相关机制。方法:胃癌细胞HGC-27和SGC-7901经终浓度为16、8、4、2、1和0 μg/ml的白扁豆多糖作用24、48和72h,各设3个复孔。MTS法检测其增殖活性;分别取经4、0 μg/ml白扁豆多糖作用24h的HGC-27和SGC-7901细胞(各3个复孔),JC-1染色观察线粒体膜电位,流式细胞仪分析细胞周期和凋亡率,QPCR法探讨Bcl-2、caspase-3和Bax基因的mRNA转录水平。结果:白扁豆多糖作用后,HGC-27和SGC-7901细胞线粒体膜电位降低;细胞增殖显著受抑制(P<0.01),且效果与药物作用浓度和时间有关;细胞凋亡率分别为53.15%和38.77%,均较PBS处理组(8.07%和6.03%)明显增加(P<0.01),而细胞周期无显著变化;同时,细胞内Bcl-2基因的转录水平明显受抑制,Bax和caspase-3基因的转录明显上调(P<0.01)。结论:白扁豆多糖可通过调节Bax-Bcl-2-caspase3通路,诱导胃癌细胞HGC-27和SGC-7901凋亡。

关键词: 白扁豆多糖, 细胞调亡, 人胃癌细胞株HGC-27, 人胃癌细胞株SGC-7901

Abstract: Objective: To investigate the effect of white lentil polysaccharides on the apoptosis of human gastric cancer cells and its related mechanism.Methods: Gastric cancer cell lines HGC-27 and SGC-7901 were treated with 16, 8, 4, 2, 1 and 0 μg/ml white lentil polysaccharides for 24, 48 and 72 hours respectively, each experiment 3 wells. The proliferation activities of gastric cancer cells were detected with CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS). HGC-27 and SGC-7901 cells were treated with 0 or 4 μg/ml white lentil polysaccharides for 24 hours respectively, each experiment 3 wells, then the changes of cell mitochondrial membrane potential were observed by JC-1 staining, cell cycle and cell apoptosis rates were evaluated with flow cytometry, the levels of mRNA expression of Bcl-2, caspase-3 and Bax genes were investigated with real-time quantitative PCR detecting system (QPCR).Results: After the gastric cancer cell lines HGC-27 and SGC-7901 were treated with white lentil polysaccharides, the mitochondrial membrane potential of the cells was significantly reduced, proliferation activities of the cells were inhibited obviously (P<0.01), and the inhibition degree was related with drug concentration and incubation time. Flow cytometry analysis showed that the apoptosis rates of HGC-27 and SGC-7901 cell lines were 53.15% and 38.77% respectively, which were significantly increased compared with PBS treatment groups (8.07% and 6.03%, P<0.01). And the cell cycle of these cells was not influenced significantly. The results of QPCR showed that Bcl-2 gene transcription level of HGC-27 and SGC-7901 cell lines was significantly down-regulated, and the transcription levels of Bax and caspase-3 genes were significantly increased (P<0.01).Conclusion: The white lentil polysaccharides can induce the apoptosis of gastric cancer cells lines HGC-27 and SGC-7901, and the mechanism of their apoptosis may be mediated by the regulation of Bax-Bcl-2-caspase3 pathway.

Key words: white lentil polysaccharides, cell apoptosis, human gastric cancer cell line HGC-27, human gastric cancer cell line SGC-7901

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