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中国应用生理学杂志 ›› 2018, Vol. 34 ›› Issue (5): 418-421.doi: 10.12047/j.cjap.5667.2018.095

• 研究论文 • 上一篇    下一篇

不同功率的激光刺激对小鼠C2C12成肌细胞耗氧率水平的影响及机制

冯钰, 程泽鹏, 孙鹏, 史仍飞   

  1. 上海体育学院运动科学学院, 上海 200438
  • 收稿日期:2018-01-03 修回日期:2018-09-25 出版日期:2018-09-28 发布日期:2019-02-21
  • 通讯作者: 史仍飞,Tel:021-65507363;E-mail:rfshi@sus.edu.cn E-mail:rfshi@sus.edu.cn
  • 基金资助:
    史仍飞,Tel:021-65507363;E-mail:rfshi@sus.edu.cn

Effects of laser stimulation with different power on oxygen consumption rate of C2C12 myoblasts in mice

FENG Yu, CHENG Ze-peng, SUN Peng, SHI Reng-fei   

  1. Academy of Sport Sciences, Shanghai University of Sport, Shanghai 200438, China
  • Received:2018-01-03 Revised:2018-09-25 Online:2018-09-28 Published:2019-02-21
  • Contact: 史仍飞,Tel:021-65507363;E-mail:rfshi@sus.edu.cn E-mail:rfshi@sus.edu.cn
  • Supported by:
    史仍飞,Tel:021-65507363;E-mail:rfshi@sus.edu.cn

摘要: 目的:探讨不同功率的低强度650 nm激光刺激对C2C12成肌细胞耗氧率水平和相关蛋白的影响及其机制。方法:以体外培养的C2C12小鼠成肌细胞作为实验对象,以4×105个/孔接种于牵张6孔板中,采用输出功率5 mW,波长650 nm的二极管激光进行单次刺激,激光照射剂量分别0 J/cm2(0 min)、0.4 J/cm2(12.8 min)、0.8 J/cm2(25.6 min)。实验结束后,采用耗氧率试剂盒(Luxcel Biosciences)检测细胞耗氧率;提取细胞总蛋白,采用Western blot技术检测成肌调节因子(MyoD)、过氧化物酶体增殖活化受体γ共激活因子1α(PGC-1α)、雷帕霉素靶蛋白和磷酸化蛋白(p-mTOR/mTOR)表达。结果:与对照组相比,低剂量组细胞氧化耗氧率结果、MyoD、PGC-1α蛋白表达显著增加(P<0.05),高剂量组MyoD、PGC-1α蛋白表达显著增加(P<0.05),p-mTOR/mTOR蛋白显著降低(P<0.05)。结论:较低剂量(0.4 J/cm2)的650 nm低强度激光增强了细胞氧化功能水平,并对细胞分化相关蛋白有一定影响。其机制可能与适宜的激光刺激影响PGC-1α蛋白的表达,进而影响线粒体氧化呼吸有关。

关键词: 低强度激光, 耗氧率, PGC-1α, 小鼠C2C12成肌细胞

Abstract: Objective: To investigate the effects of 650 nm laser irradiation on cell oxygen consumption rate in C2C12 myoblasts following different doses. Methods: C2C12 cells were irradiated with 650 nm laser(λ=650 nm, p=5 mW) with energy densities of 0, 0.4, and 0.8 J/cm2. Cell oxidative function was measured by oxygen consumption rate kit. Protein expression of myogenic determination factor (MyoD), peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), mammalian target of rapamycin (mTOR) and its phosphorylation were detected by Western blot. Results: Compared to the control group, the expression levels of MyoD, PGC-1α protein were increased and cell oxygen consumption rate was promoted in the low dose group(P<0.05). MyoD and PGC-1α protein expressions were also increased(P<0.05), the ratio of mTOR and its phosphorylationwere decreased significantly in the high dose group(P<0.05). Conclusion: 650 nm laser irradiation that dose is 0.4 J/cm2 enhances cell oxidative function, it related to that proper dose laser irradiation promoted the expression of PGC-1a protein.

Key words: laser irradiation, oxygen consumption rate, PGC-1α, C2C12 cells

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