纳米炭黑颗粒复合寒冷暴露对小鼠肺部氧化应激反应的影响

doi:10.12047/j.cjap.5669.2018.061

中国应用生理学杂志 ›› 2018, Vol. 34 ›› Issue (3): 258-262.doi: 10.12047/j.cjap.5669.2018.061

纳米炭黑颗粒复合寒冷暴露对小鼠肺部氧化应激反应的影响

赵丹丹^1,2, 郭展², 李曦², 张永强², 张莉², 朱五福¹, 郑鹏武¹, 杨丹凤²

1. 江西科技师范大学药学院南昌 330013;
2. 军事科学院军事医学研究院环境医学与作业医学研究所, 天津 30050

收稿日期:2018-01-08 修回日期:2018-02-14 出版日期:2018-05-28 发布日期:2018-09-08
通讯作者: 郑鹏武,Tel:022-84655058;E-mail:zhengpw@126.com;杨丹凤,E-mail:fengdyd@126.com E-mail:zhengpw@126.com;fengdyd@126.com
基金资助:
国家自然科学基金资助（21707165），BWS16J0101

Effects of nano-carbon black particles combined with cold exposure on oxidative stress of lung in mice

ZHAO Dan-dan^1,2, GUO Zhan², LI Xi², ZHANG Yong-qiang², ZHANG Li², ZHU Wu-fu¹, ZHENG Peng-wu¹, YANG Dan-feng²

1. School of Pharmacy, Jiangxi Science and Technology Normal University, Nanchang 330013;
2. Institute of Environmental and Operational Medicine, Institute of Military Medicine, Academy of Military Sciences, Tianjin 30050, China

Received:2018-01-08 Revised:2018-02-14 Online:2018-05-28 Published:2018-09-08
Supported by:
国家自然科学基金资助（21707165），BWS16J0101

摘要/Abstract

摘要： 目的：研究纳米炭黑颗粒复合寒冷暴露对小鼠肺部组织结构及其氧化应激反应的影响。方法：将72只健康雄性C57BL/6小鼠随机分为6组：对照（Ctrl）组、单纯冷暴露（C）组、低剂量染毒（L）组、低剂量染毒复合冷暴露（LC）组、高剂量染毒（H）组、高剂量染毒复合冷暴露（HC）组。采用吸入式气管滴注染毒方式，一次性滴注纳米炭墨颗粒染毒液40 μl，浓度分别为0.45 mg/ml （L）和4.05 mg/ml （H）。冷暴露方式为4℃暴露，4 h/d，连续20 d。暴露结束24 h后称重、取样，进行相关指标测定。采用试剂盒法测定小鼠肺组织匀浆中超氧化物歧化酶（SOD）活力、谷胱甘肽过氧化物酶（GSH-Px）活力和丙二醛（MDA）含量；肺组织块HE染色，观察肺组织病理组织结构改变。结果：所有冷暴露处理组小鼠的体重均显著低于所有非冷暴露组（P<0.05），对照组及单纯染毒组小鼠体重均在实验开始14 d后明显升高（P<0.05），单纯冷暴露组与纳米炭黑颗粒染毒复合冷暴露组小鼠体重均在14 d后趋于稳定。HE检测结果表明，单纯纳米炭黑颗粒染毒组及染毒复合冷暴露组小鼠肺泡腔内均有黑色颗粒沉积，高剂量染毒复合冷暴露组可见肺泡结构破环，排列凌乱，有大量炎细胞浸润。与对照组相比，其余各组SOD活力均显著降低（P<0.05）；高剂量染毒组及高剂量染毒复合冷暴露组GSH-Px活力明显低于对照组（P<0.01）；与对照组相比，高剂量染毒组、低剂量染毒与高剂量染毒复合冷暴露组MDA含量显著升高（P<0.01）。两因素方差分析提示，随着染毒剂量的增加，SOD活力及GSH-Px活力显著降低（P<0.05）；随着温度的降低，肺组织MDA含量显著升高（P<0.05），4℃间歇性冷暴露与纳米颗粒物暴露对肺组织SOD、GSH-Px活力及MDA含量的影响均无交互作用。结论：纳米炭黑颗粒复合寒冷暴露可导致小鼠肺部炎症反应加重，氧化应激水平升高。

关键词: 寒冷暴露, 纳米炭黑颗粒物, 小鼠, 肺, 氧化应激

Abstract: Objective: To study the effects of nano-carbon black particles combined with cold exposure on lung tissue and its oxidative stress in mice.Methods: Seventy-two healthy male C57BL/6 mice were randomly divided into six groups:control group (Ctrl), cold exposure group (C), low dose exposure group (L), low dose exposure combined with cold exposure group (LC), high dose exposure group (H), high dose exposure combined with cold exposure group (HC). The suspensions of nanoscale carbon black particles were intratracheal instilled to mice once time, the exposure doses were 0.45 mg/ml (L) and 4.05 mg/ml (H). The mice in cold group and in nano-carbon black particles exposure combined with cold exposure groups had been intermittently exposed to 4℃ for 20 days (4 h/day). Then the mice were weighed and the lung were drawn. The activities of SOD (superoxide dismutase), GSH-Px (glutathione peroxidase) and the content of MDA (malondialdehyde) in lung were determined. The lung pathological slices were prepared to observe the structural changes of the lung.Results: All cold exposed mice were significantly lighter in body weight than mice in all non-cold-exposed groups(P<0.05),the body weight of the mice in control group and in simple nano-carbon black particles exposure group began to increase significantly after the 14th day of the experiment(P<0.05), the body weight of the mice in the C group, LC group and HC group tended to be stable after the 14th day of the experiment. The results of HE pathological examination showed that the alveolar spaces of mice in L group, LC group, H group and HC group were all deposited with black granules. The alveolar structure was disrupted, the arrangement was in disorder, and a large number of inflammatory cells infiltrated in lung of HC group. Compared with the control group,SOD activity of the other groups was significantly decreased(P<0.05). The GSH-Px activity of the high-dose exposure group and the high-dose exposure combined with cold exposure group was significantly lower than that of the control group (P<0.01). The MDA content in H group, LC group and HC group was significantly higher than that of the control group(P<0.01). The two factor variance analysis showed that as the administrated dose increased,SOD activity and GSH-Px activity were decreased significantly(P<0.05), and as the decrease of temperature, the MDA content of lung tissue was significantly increased(P<0.05). There was no interaction effect between 4℃ intermittent cold exposure and nano-carbon black particles exposure on the activity of SOD, GSH-Px and the content of MDA in lung tissue.Conclusion: Nano-carbon black particles combined with cold exposure might aggravate lung inflammation and increase the level of oxidative stress in mice.

Key words: cold exposure, nano-carbon black particles, mouse, lung, oxidative stress

赵丹丹, 郭展, 李曦, 张永强, 张莉, 朱五福, 郑鹏武, 杨丹凤. 纳米炭黑颗粒复合寒冷暴露对小鼠肺部氧化应激反应的影响[J]. 中国应用生理学杂志, 2018, 34(3): 258-262.

ZHAO Dan-dan, GUO Zhan, LI Xi, ZHANG Yong-qiang, ZHANG Li, ZHU Wu-fu, ZHENG Peng-wu, YANG Dan-feng. Effects of nano-carbon black particles combined with cold exposure on oxidative stress of lung in mice[J]. CJAP, 2018, 34(3): 258-262.

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纳米炭黑颗粒复合寒冷暴露对小鼠肺部氧化应激反应的影响

Effects of nano-carbon black particles combined with cold exposure on oxidative stress of lung in mice

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