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中国应用生理学杂志 ›› 2019, Vol. 35 ›› Issue (1): 47-49.doi: 10.12047/j.cjap.5690.2019.012

• 研究论文 • 上一篇    下一篇

参麦注射液对严重烫伤大鼠肠道屏障功能的保护作用

杨晨1,宋垚垚2,季一发3△   

  1. 1. 中国人民武装警察部队特色医学中心烧伤整形科, 天津 300162;
    2. 解放军总医院第六医学中心烧伤科, 北京 100048;
    3. 北京丰台右安门医院美容整形科, 北京 100069
  • 收稿日期:2018-04-02 出版日期:2019-01-28 发布日期:2019-06-27
  • 通讯作者: Tel: 010-83322000; E-mail: 2811894982@qq.com
  • 基金资助:
    解放军总医院第六医学中心创新培育基金(CXPY201732)

Protective effects of Shenmai injection on intestinal mucosal barrier function in severely scalded rats

YANG Chen1, SONG Yao-yao2, JI Yi-fa3△   

  1. 1. Department of Burn and Plastic Surgery, Special Medical Center of Chinese People’s Armed Police Force, Tianjin 300162;
    2. Department of Burn and Plastic Surgery, the Sixth Medical Center of PLA General Hospital, Beijing 100048;
    3. Department of Cosmetic Surgery, Beijing Fengtai Youanmen Hospital, Beijing 100069, China
  • Received:2018-04-02 Online:2019-01-28 Published:2019-06-27

摘要: 目的:探索参麦注射液对30% Ⅲ°烫伤早期肠道屏障功能的保护作用,为参麦注射液防治肠源性感染提供实验依据。方法:Wistar大鼠60只,随机分为正常对照组、模型对照组,地塞米松5 mg/kg组、参麦注射液5、10、15 mg/kg组,每组10只,使用烫伤仪建立30% Ⅲ°烫伤动物模型,立即腹腔注射相应的药物,每天1次。烫伤72 h后,检测肝脏、脾脏、肠系膜淋巴结细菌移位量、血浆内毒素、二胺氧化酶(DAO)、肿瘤坏死因子-α(TNF-α)及白细胞介素-6(IL-6)水平和肠粘膜分泌型免疫球蛋白A(sIgA)的水平。结果:与正常对照组比较,模型组肝脏、脾脏、肠系膜淋巴结细菌移位量,血浆内毒素、DAO、TNF-α及 IL-6和肠黏膜sIgA水平明显升高(P<0.01);与模型组比较,地塞米松组和参麦注射液5、10、15 mg/kg组肝脏、脾脏、肠系膜淋巴结细菌移位量,血浆内毒素、DAO、TNF-α及 IL-6和肠黏膜sIgA水平明显降低(P<0.05或P<0.01)。结论:参麦注射液可减轻严重烫伤引起的肠粘膜损伤,效果与地塞米松相当,高剂量组效果更好。

关键词: 烫伤, 肠黏膜屏障, 细菌易位, 内毒素, 分泌性免疫球蛋白A, 大鼠

Abstract: Objective:To investigate the protective effects of shen-mai injection on intestinal barrier function in the early stage of 30% Ⅲ° scald, and to provide experimental basis for the prevention and control of enterogenic infection. Methods: A total of 60 Wistar rats were randomly divided into 6 groups: normal control group (without treatment), model control group (with 30% total body surface area (TBSA) fully thickness burn on the back), hexadecadrol (5 mg/kg) group, Shenmai injection (5, 10, 15 mg/kg) groups, with 10 rats in each group. After burned by scald apparatus, rats in each group were treated with drugs immediately by intraperitoneal injection once a day. At 72 hours after burned, the levels of plasma endotoxin, diamine oxidase (DAO), tumor necrosis factor-alpha (TNF-α), interleukins-6(IL-6) in all rats were detected and the mesenteric lymph nodes, liver and spleen were homogenized to culture for bacteria. The change of secretory immunoglobulin A (sIgA) in intestinal mucosa was measured. Results: Compared with normal control group, bacterial translocation quantity in mesenteric lymph nodes(MLN), liver, and spleen, and the plasma levels of DAO, endotoxin, TNF-α, IL-6 and the level of sIgA in intestinal mucosa in model control group were increased significantly (P<0.01); compared with model control group, bacterial translocation quantity in MLN, liver, and spleen, and the plasma levels of DAO, endotoxin, TNF-α, IL-6 and the level of sIgA in intestinal mucosa in hexadecadrol (5 mg/kg) group and shen-mai injection (5, 10, 15 mg/kg) groups were decreased significantly (P<0.05 or P<0.01). Conclusion: Shen-mai injection can alleviate intestinal mucosa injury caused by severe scald, and the effects are similar with those of dexamethasone, and the effect is better in the high-dose group.

Key words: scald, intestinal mucosal barrier, bacterial translocation, endotoxin, sIgA, rats

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