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中国应用生理学杂志 ›› 2019, Vol. 35 ›› Issue (1): 55-59.doi: 10.12047/j.cjap.5706.2019.014

• 研究论文 • 上一篇    下一篇

枸杞多糖对糖尿病大鼠视网膜神经元的保护作用及其机制

潘虹1△,施真1,杨泰国2,于腊梅1,徐爱丽1   

  1. 1. 滨州医学院生理学教研室, 山东 烟台 264003;
    2. 平邑县人民医院骨科, 山东 平邑 273300
  • 收稿日期:2018-05-27 出版日期:2019-01-28 发布日期:2019-06-27
  • 通讯作者: Tel: 0535-6913210; E-mail: pjlndy@163.com
  • 基金资助:
    国家自然科学基金青年科学基金项目(81700865);山东省自然科学基金(ZR2018MC011,BS2015YY036)

The protective effects of lycium barbarum polysaccharides on retinal neurons in diabetic rats and its mechanism

PAN Hong1△, SHI Zhen1, YANG Tai-guo2, YU La-mei1, XU Ai-li1   

  1. 1. Department of Physiology, Binzhou Medical College, Yantai 264003;
    2. The People's Hospital of Pingyi County, Pingyi 273300, China
  • Received:2018-05-27 Online:2019-01-28 Published:2019-06-27

摘要: 目的:观察枸杞多糖(LBP)对糖尿病大鼠视网膜神经细胞的保护作用,并探讨其作用机制。方法:18只SD大鼠随机分为3组(n= 6):正常对照组(NC),糖尿病模型组(DM)和LBP治疗组(DM+LBP),通过一次性腹腔注射链脲佐菌素(STZ)的方法制备糖尿病大鼠模型。DM+LBP组按1 mg/(kg·d)剂量的LBP灌胃12周。治疗结束后检测大鼠体重、空腹血糖、视网膜活性氧簇(ROS)的生成、视网膜神经节细胞(RGCs)和无长突细胞的表达、视网膜NF-E2相关因子2(Nrf2)和血红素加氧酶-1(HO-1)的蛋白表达。结果:STZ诱导糖尿病大鼠模型造模成功率100%。与NC 组相比,DM组大鼠体重明显降低、空腹血糖值升高、ROS的生成明显增加、RGCs和无长突细胞的数量均明显减少(P<0.01)。与DM组相比,LBP治疗组大鼠体重升高、血糖降低、ROS的生成减少、RGCs和无长突细胞的数量均明显增加(P<0.01或P<0.05);视网膜Nrf2和HO-1的蛋白表达均明显升高(P<0.01)。结论:LBP能改善糖尿病大鼠视网膜的氧化应激状态,对糖尿病大鼠视网膜神经细胞有一定的保护效应,其作用机制可能与其激活Nrf2/HO-1信号通路有关。

关键词: 枸杞多糖, 大鼠, 糖尿病视网膜病变, 氧化应激, NF-E2相关因子2, 血红素加氧酶-1

Abstract: Objective:To clarify whether lycium barbarum polysaccharides (LBP) have protective effects on retina neuronal cells in diabetic rats and to identify the related mechanism involved in this process. Methods: Eighteen SD rats were randomly divided into 3 groups ( n= 6): normal control group (NC), diabetes mellitus group (DM) and LBP-treatment group (DM+LBP). The diabetic rat model was induced by single intraperitoneal injection of streptozotocin (STZ). The rats in DM+LBP group were treated with LBP at the dose of 1 mg/kg by gavage, once a day for 12 weeks. After the treatment, the weight and blood glucose, the generation of reactive oxygen species (ROS), the surviving retinal ganglion cells (RGCs) and amacrine cells and the protein expressions of nuclear factor E2-related factor 2 (Nrf2) and the heme oxygenase-1 (HO-1) were detected. Results: The successful rate of diabetic model was 100%. Compared with NC group, the rats of DM group caused weight loss, elevated blood glucose, a marked increase of ROS generation and a significant decrease in the number of RGCs and amacrine cells (P<0.01), and these effects were diminished or abolished by LBP treatment. Meanwhile, LBP significantly increased the expressions of Nrf2 and HO-1 in the retinas of diabetic rats (P<0.01). Conclusion: LBP can improve retinal oxidative stress and exert beneficial neuroprotective effects in diabetic rats, and its mechanism may be associated with the activation of the Nrf2/HO-1 antioxidant pathway.

Key words: lycium barbarum polysaccharides, rat, diabetic retinopathy, oxidative stress, nuclear factor E2-related factor 2, heme oxygenase-1

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