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中国应用生理学杂志 ›› 2019, Vol. 35 ›› Issue (3): 228-231.doi: 10.12047/j.cjap.5763.2019.049

• 研究论文 • 上一篇    下一篇

人参皂苷Rg2对慢性坐骨神经损伤大鼠痛觉敏化和抑郁状态的影响*

张巧莲, 李双英, 李平   

  1. 中国人民武装警察部队特色医学中心神经内科, 天津 3001062
  • 出版日期:2019-05-28 发布日期:2019-06-28
  • 通讯作者: Tel: 022-60577115; E-mail: liping99038@163.com

Effects of ginsenoside-Rg2 on mechanical allodynia, heat hyperalgeia, depressive state of rats with chronic sciatic nerve constriction injury

ZHANG Qiao-lian, LI Shuang-ying, LI PING   

  1. Department of Neurology, Characteristic Medical Center of the Chinese People’s Armed Police Forces, Tianjin 300162, China
  • Online:2019-05-28 Published:2019-06-28

摘要: 目的:观察人参皂苷Rg2对慢性坐骨神经损伤大鼠痛觉敏化、抑郁状态的影响。方法: 将50只 SD 大鼠随机分为 5组(n=10): 空白对照组(Normal+生理盐水腹腔注射)、假手术组(手术但不结扎+生理盐水腹腔注射) 、坐骨神经慢性压迫损伤(CCI)组(CCI +生理盐水腹腔注射) 、人参皂苷Rg2低剂量组(CCI+ Rg2 5 mg/kg腹腔注射)、人参皂苷Rg2高剂量组(CCI+ Rg2 10 mg/kg 腹腔注射)。CCI模型建立后,药物通过注射器进行腹腔内注射 5 ml/kg,每天1次,连续14 d。分别在术前1 d和术后 1、3、5、7、10、14 d测定大鼠的机械性缩足反射阈值(MWT)和热缩足潜伏期(TWL);术前1 d和术后第14日时检测明暗箱实验和强迫游泳试验。 结果:与假手术组比较,CCI组术后14 d机械痛阈值和热痛潜伏期明显缩短(P<0.01),明箱内停留时间明显缩短(P<0.01),穿梭次数明显减少(P<0.01),游泳潜伏期明显延长(P<0.01)。与CCI组比较,人参皂苷Rg2组术后14 d机械痛阈和热痛潜伏期明显增加(P<0.01),大鼠在明箱内时间明显延长(P<0.01),穿梭次数明显增多(P<0.01),且游泳潜伏期明显缩短(P<0.01)。结论:人参皂苷Rg2能抑制 CCI 大鼠的机械痛敏和热痛敏,同时改善其抑郁状态。

关键词: 人参皂苷Rg2, 慢性坐骨神经损伤, 机械痛敏, 热痛敏, 抑郁状态, 大鼠

Abstract: Objective: To investigate the effects of ginsenoside-Rg2 on mechanical allodynia, heat hyperalgeia, depressive state of rats with chronic sciatic nerve constriction injury. Methods: Fifty SD rats were randomly divided into 5 groups: blank control group (Normal, normal + saline),sham operation group (Sham, sham operation + saline),chronic constriction injury of the sciatic nerve group (CCI, CCI + saline),ginsenoside-Rg2 low dose group (CCI + Rg2 5 mg/kg), and ginsenoside-Rg2 high dose group (CCI + Rg2 10 mg/kg).After the CCI model was established,drug were injected into the abdominal cavity through the syringe once a day,for 14 consecutive days.The mechanical shrinkage foot reflex threshold (MWT) and thermal withdrawal latency(TWL) were determined at 1 d before the operation and at 1,3,5,7,10 and 14 d after the operation.Light-dark transition test, forced swimming test were determined at 1 d before the operation and at 14 d after the operation.Results: Compared with the sham group, the MWL and TWL of the CCI rats were decreased significantly (P<0.01), time in the light compartment and number of transition were decreased (P<0.01), the immobility time in FST was also prolonged significantly (P<0.01). At 14 days after CCI operation, the MWL and TWL of the ginsenoside-Rg2 groups were increased significantly (P<0.01), time in the light compartment and number of transition were also shortened significantly (P<0.01), the immobility time in FST was also shortened significantly (P<0.01). Conclusion: Intraperitoneal injection of ginsenoside-Rg2 can inhibit the mechanical and thermal pain sensitivity of CCI rats,and can relieve depressive state.

Key words: ginsenoside-Rg2, chronic constriction injury of the sciatic nerve, mechanical allodynia, heat hyperalgeia, depressive state, rat

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