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中国应用生理学杂志 ›› 2019, Vol. 35 ›› Issue (4): 366-370.doi: 10.12047/j.cjap.5779.2019.078

• 研究论文 • 上一篇    下一篇

石菖蒲及其活性成分-5-羟甲基糠醛对疲劳运动大鼠学习记忆的影响及其机制*

陈惠花1, 朱梅菊1△, 朱洪竹1, 丁孝民1, 王卉1, 毛泽华2   

  1. 1. 井冈山大学体育学院, 江西 吉安 343009;
    2. 井冈山大学后勤保障处, 江西 吉安 343009
  • 收稿日期:2018-11-12 出版日期:2019-07-28 发布日期:2019-11-06
  • 通讯作者: ,Tel: 0796-8100491; E-mail: zhu_mj@163.co m
  • 基金资助:
    * 国家自然科学基金项目(31660291,31360255,31160217);江西省自然科学基金资助项目(20151BAB205070);井冈山大学博士启动基金资助项目(JZB11043)

Effects of Acorus tatarinowii Schott and its active component -5- hydroxymethylfurfural on ERK/CREB signal in hippocampus of exercise-induced fatigue rats

CHEN Hui-hua1, ZHU Mei-ju1△, ZHU Hong-zhu1, DING Xiao-min1, WANG Hui 1, MAO Ze-hua2   

  1. 1. Institute of Physical Education, Jinggangshan University, Ji’an 343009;
    2. Logistics Support Department, Jinggangshan University, Ji’an, 343009, China
  • Received:2018-11-12 Online:2019-07-28 Published:2019-11-06

摘要: 目的:研究石菖蒲及5-羟甲基糠醛(HMF)对疲劳运动大鼠学习记忆和海马ERK/CREB信号的影响。方法:将SD大鼠随机分为:正常组(A)、运动组( B )、运动+HMF低、中、高剂量组(C、D、E)、运动+石菖蒲低、中、高剂量组( F、G、H ),每组10只。 并在疲劳运动开始前2 h分别以0.10、1.00和3.00 mg. kg-1. wt-1 HMF,灌胃C、D、E组,以0.12、1.20和4.80 g. kg-1. wt-1石菖蒲提取物,灌胃 F、G、H组。实验结束后采用水迷宫实验进行学习记忆检测,用免疫印迹法测定海马p-ERK1/2和p-CREB表达。结果:E和H组大鼠逃避潜伏期低于B、C、D、F和G组;穿越平台次数、p-ERK1/2、p-CREB蛋白表达高于B、C、D、F和G组(P均<0.01);除E组p-ERK2蛋白表达低于A、H组(P<0.05)外,上述各指标A、E、H组比较,差异均无显著性(P均>0.05)。结论:石菖蒲及HMF能明显改善运动疲劳大鼠学习记忆,其机制与上调海马ERK/CREB信号有关。

关键词: 石菖蒲, 5-羟甲基糠醛, 运动, 学习记忆, 细胞外信号调节激酶, cAMP 反应元件结合蛋白, 大鼠

Abstract: Objective: To investigate the effects of Acorus tatarinowii Schott and its active component 5- hydroxymethyl furfural (HMF) on learning and memory and ERK/CREB signal in hippocampus of rats with exercise-induced fatigue. Methods: SD rats were randomly divided into normal group (A), exercise group (B), exercise + HMF low, middle and high dose treatment group (C, D, E), exercise + acorus tatarinowii Schott low, middle and high dose treatment group (F, G, H), with ten rats in each group. The rats in group C, D and E were treated with HMF at the doses of 0.10, 1.00 and 3.00 mg. kg-1 by ig. The rats in group F, G and H were treated with the extracts of Acorus tatarinowii Schott at the doses of 0.12, 1.20 and 4.80 g. kg-1 by ig. Learning and memory of rats were tested by the method of water maze experiment, and the expression levels of p-ERK1/2 and p-CREB protein in hippocampus of rats were tested by the method of Western blot in the end of the experiment. Results: The escape latencies of E and H groups were lower than those of groups B, C, D, F and G; and the numbers of plateau crossing were more than those of groups B, C, D, F and G and the expression levels of p-ERK1/2, p-CREB protein were higher than those of groups B, C, D, F and G , respectively(P < 0.01). There was no significant difference in the above indexes among groups A, E and H(P>0.05) except that the expression levels of p-ERK2 protein in group E were lower than those in group A and H (P<0.05). Conclusion: Acorus tatarinowii and its active component- HMF can improve the learning and memory of rats with exercise-induced fatigue, and the mechanism is related to the up-regulation of ERK / CREB signal in hippocampus of rats with exercise-induced fatigue.

Key words: acorus tatarinowii Schott, 5-hydroxymethylfurfural, exercise, learning and memory, extracellular signal-regulated kinase, cAMP-responsive element-binding protein, rat

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