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中国应用生理学杂志 ›› 2019, Vol. 35 ›› Issue (4): 351-354.doi: 10.12047/j.cjap.5785.2019.074

• 研究论文 • 上一篇    下一篇

红花水提取液对小鼠系统性硬皮病的防治作用及其机制*

范春芳1, 张红霞1, 唐逸豪1, 徐海环2, 宋冬3△   

  1. 1. 中国人民武装警察部队特色医学中心药剂科, 天津 300162;
    2. 中国人民武装警察部队特色医学中心皮肤科, 天津 300162;
    3. 中国人民武装警察部队特色医学中心, 天津 300162
  • 收稿日期:2018-11-20 出版日期:2019-07-28 发布日期:2019-11-06
  • 通讯作者: ,Tel: 18910366626; E-mail: 18910366626@163.com

Preventive and therapeutic effects of safflower water extract on systemic scleroderma in mice and its mechanism

FAN Chun-fang1, ZHANG Hong-xia1, TANG Yi-hao1, XU Hai-huan2, SONG Dong3△   

  1. 1. Pharmacy Department of Characteristic Medical Center of the Chinese People’s Armed Police Force, Tianjin 300162;
    2. Dermatology Department of Characteristic Medical Center of the Chinese People’s Armed Police Force, Tianjin 300162;
    3. Characteristic Medical Center of the Chinese People's Armed Police Force, Tianjin 300162, China
  • Received:2018-11-20 Online:2019-07-28 Published:2019-11-06

摘要: 目的:研究红花水提取液对系统性硬皮病(SSc)模型小鼠的防治作用及相关机制研究。方法:60只 BALB /C小鼠随机分为对照组、模型组、强的松组、红花低、中、高剂量组,每组10只。对照组背部注射生理盐水,其余5组均背部皮下注射100 μl浓度为 200 μg /ml的注射用盐酸博来霉素,每天1次,连续注射28 d,制备SSc模型;造模同时对照组和模型组给予生理盐水10 ml/kg灌胃,强的松组给予强的松溶液4.5 mg/kg (10 ml/kg)灌胃,红花低、中、高剂量组分别给予红花1.5、3、6 g/kg (10 ml/kg)灌胃,各组均连续灌胃28 d。给药28 d后,取各组小鼠背部注射博来霉素区皮肤组织切片测量真皮厚度,采用水解法检测皮肤组织羟脯氨酸(HYP)含量;采用ELISA法检测皮肤组织结缔组织生长因子(CTGF)、转化生长因子-β(TGF-β)含量及血清白细胞介素-6(IL-6)、白细胞介素-17(IL-17)水平。结果:与对照组比较,模型组皮肤真皮厚度,皮肤组织CTGF、TGF-β、HYP含量及血清 IL-6、IL-17 水平明显升高(P<0.05);与模型组比较,强的松组、红花低、中、高剂量组皮肤真皮厚度,皮肤组织 CTGF、TGF-β、HYP含量及血清 IL-6、IL-17水平明显降低(P<0.05)。结论:红花水提取液可改善SSc小鼠皮肤状况(或真皮厚度),其作用机制可能与减轻免疫炎症反应有关。

关键词: 系统性硬皮病, 博莱霉素, 结缔组织生长因子, 转化生长因子-β, 羟脯氨酸, 小鼠

Abstract: Objective: To study the preventive and therapeutic effects of safflower water extract on systemic scleroderma (SSc) in mice and its mechanism.Methods: Sixty BALB/C mice were randomly divided into the control group, model group, prednisone group and safflower low, middle, high dose groups, 10 mice in each group.The control group was injected with normal saline, and the other five groups were subcutaneously injected with bleomycin hydrochloride with 100 μl at the concentration of 200 μg /ml on the back, once a day for 28 days to establish the SSc models.At the same time, the control group and model group were treated with normal saline (10 ml/kg), the prednisone group was treated with prednisone 4.5 mg/kg (10 ml/kg), and the low, middle, and high dose safflower groups were treated with safflower at the doses of 1.5, 3, 6 g/kg (10 ml/kg), and all groups were treated for 28 days.After 28 days, all mice were decapitated. The blood samples and back skin of the BLM injection part were collected.After that, all the tissue slices were taken to measure the dermal thickness, and the content of hydroxyproline (HYP) in the skin tissues was detected by hydrolysis method.The contents of tissue growth factor (CTGF) and transforming growth factor-β (TGF-β ) in the skin tissues and the levels of interleukin-6 (IL-6) and interleukin-17 (IL-17) in serum were determined by ELISA.Results: Compared with the control group, the dermal thickness of the model group was increased(P<0.05), the contents of CTGF, TGF-β and HYP in the skin tissues and the levels of IL-6 and IL-17 in the serum of the model group were increased(P<0.05); compared with the model group, the dermal thickness in the prednisone group and safflower groups was decreased (P<0.05), the levels of CTGF, TGF-β and HYP in the skin tissues and the serum levels of IL-6 and IL-17 in the prednisone group and safflower groups were decreased (P<0.05).Conclusion: Safflower water extract can improve skin condition (or dermal thickness) in SSc mice, and its mechanism may be related to reducing immune inflammatory response.

Key words: systemic scleroderma, bleomycin, CTGF, TGF-β, hydroxyproline, mouse

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