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中国应用生理学杂志 ›› 2020, Vol. 36 ›› Issue (2): 184-188.doi: 10.12047/j.cjap.5847.2020.041

• 研究论文 • 上一篇    下一篇

α-烯醇化酶基因干扰表达对籽鹅卵泡颗粒细胞增殖及凋亡的影响*

计红1, 邵子益1, 薛琳琳2, 牛春阳1, 詹雪龙1, 杨闯1, 甄莉1, 杨焕民1, 李士泽1△   

  1. 1.黑龙江八一农垦大学动物科技学院, 黑龙江 大庆 163319,
    2.黑龙江职业学院, 黑龙江 双城 1501113
  • 出版日期:2020-03-28 发布日期:2020-07-31
  • 通讯作者: Tel: 15214611935; E-mail: 2262962424@qq.com
  • 基金资助:
    *国家自然科学基金项目(31372398);黑龙江省自然科学基金重点项目(ZD201116)

Effects of α-enolase gene interference expression on proliferation and apoptosis of follicular granulosa cells from Zi geese

JI Hong1, SHAO Zi-yi1, XUE Lin-lin2, NIU Chun-yang1, ZHAN Xue-long1, YANG Chuang1, ZHEN Li1, YANG Huan-min1, LI Shi-ze1△   

  1. 1. College of Animal Science and Veterinary Medicine, HeilongjiangBayi Agricultural University, Daqing 163319;
    2. Heilongjiang Polytechnic, Shuangcheng 150111, China
  • Online:2020-03-28 Published:2020-07-31

摘要: 目的: 研究α-烯醇化酶(ENO1)基因干扰表达对籽鹅卵泡颗粒细胞增殖、凋亡和细胞周期的影响。方法: 原代培养籽鹅F1级卵泡颗粒细胞(复合培养),将ENO1基因干扰表达重组质粒转染至籽鹅卵泡颗粒细胞。实验分为四组:ENO1干扰表达组(RNAi)、无关序列干扰组(NC)、培养液组(Control)、转染试剂组(Lip)。流式细胞术检测干扰组和对照各组的凋亡率、细胞周期时相性。结果: ENO1基因干扰表达使籽鹅卵泡颗粒细胞增殖速度变慢,凋亡率增加,G2/M期颗粒细胞比例增高。结论: ENO1基因干扰表达可使籽鹅卵泡颗粒细胞周期发生G2/M期阻滞,诱导细胞发生凋亡,抑制细胞增殖。

关键词: α-烯醇化酶, 籽鹅, 卵泡颗粒细胞, 增殖, 凋亡, 细胞周期

Abstract: Objective: To study the effects of α-enolase (ENO1) gene interference expression on proliferation, and cell cycle of follicular granulosa cells from Zi geese. Methods: F1 follicular granulosa cells were primary cultured (mixed culture), which were divided into four groups: ENO1 interference expression group (RNAi), unrelated sequence group (NC), culture group (Control), transfection reagent group (Lip). The apoptosis rate and cell cycle phase of the interference group and the control group were detected by the flow cytometry. Results: ENO1 gene interference expression slowed the proliferation of granulosa cells, increased the apoptosis, and increased the proportion of granulosa cells in G2/M phase. Conclusion: ENO1 gene interference expression could cause G2/M phase arrest in primary cultured goose follicular granulosa cells, induce cell apoptosis and inhibit cell proliferation.

Key words: α-enolase, Zi goose, follicular granulosa, proliferation, apoptosis, cell cycle

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