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中国应用生理学杂志 ›› 2019, Vol. 35 ›› Issue (6): 496-500.doi: 10.12047/j.cjap.5852.2019.108

• 研究论文 • 上一篇    下一篇

二氢杨梅素改善慢性社会挫败应激小鼠认知与情感障碍*

王乐1, 李碧蓉1, 肖志勇2, 赵金龙1, 于旭东1△   

  1. 1. 邵阳学院基础医学院, 湖南 邵阳 422000;
    2. 南华大学附属第一医院, 湖南 衡阳 421001
  • 收稿日期:2019-03-26 出版日期:2019-11-28 发布日期:2020-04-02
  • 通讯作者: 湖南省教育厅一般项目(09CD47);湖南省自然科学基金项目(2019JJ50548)

Dihydromyricetin ameliorates chronic social defeat stress induced cognitive and affective disorder in mice

WANG Le1, LI Bi-rong1, XIAO Zhi-yong2, ZHAO Jin-long1, YU Xu-dong1△   

  1. 1. Basic medical school, Shao yang University, Shao yang 422000;
    2. The First Affiliated Hospital, University of South, Hengyang 421001, China
  • Received:2019-03-26 Online:2019-11-28 Published:2020-04-02

摘要: 目的:探索二氢杨梅素(DHM)对慢性社会挫败应激小鼠认知与情感障碍的作用及其可能机制。方法:将C57BL/6J小鼠随机分成对照组(Control)、慢性社会挫败应激组(CSDS)和慢性社会挫败应激+DHM组(CSDS+DHM),每组14只,每天将两个应激组小鼠放入ICR攻击鼠的饲养笼中10 min,之后取出放于ICR攻击鼠饲养笼的旁边笼中,连续应激10 d,在应激5 d后,每天按10 ml/kg的量分别腹腔注射一次2%的DMSO或20 mg/kg的DHM(分散于2% DMSO中),连续注射5 d,之后每组取10只小鼠进行新颖物体识别测试、Y迷宫测试、社会交互和旷场测试、行为学测试,剩余4只小鼠于实验结束后24 h内断头取脑,采用Western blot法检测海马组织SIRT1水平。结果:与Control组比较,CSDS组小鼠的学习记忆显著降低,焦虑水平显著升高,在悬尾测试(TST)和强迫游泳测试(FST)中的不动时间显著升高,海马SIRT1蛋白水平显著降低(P均<0.05或P<0.01);与CSDS组比较,CSDS+DHM组小鼠学习记忆显著提高,小鼠焦虑水平显著降低,在TST和FST中不动时间显著降低,海马SIRT1蛋白水平显著升高(P均<0.05或P<0.01)。结论:DHM可改善CSDS诱导小鼠的认知障碍、焦虑样行为和抑郁样行为,并提高海马SIRT1蛋白的表达水平。

关键词: 二氢杨梅素, 慢性社会挫败应激, 认知功能障碍, 抑郁, 焦虑, SIRT1, 小鼠

Abstract: Objective: To investigated the effects of dihydromyricetin on cognitive and affective disorders induced by chronic social defeat stress and its possible mechanism in mice. Methods: C57BL/6J mice were randomly divided into control group (Control), chronic social defeat stress group (CSDS) and chronic social defeat stress + DHM group (CSDS+DHM) (14 mice in each group). The mice received chronic social defeat stress and were injected with DHM or vehicle intraperitoneally. A part of mice were subjected to (10 mice of each group) novel object recognition test (NOR), Y maze test, open field test (OFT), social interaction test (SIT), forced swimming test (FST) and tail suspension test (TST). The other mice (4 mice of each group) were decapitated and the expression levels of SIRT1 in hippocampus were detected by Western blot. Results: Compared with the control group, the learning and memory of the CSDS group were reduced significantly, the anxiety level was increased significantly, the immobility time in TST and FST was increased significantly, and the SIRT1 protein level in hippocampus was reduced significantly (P< 0.05 or P< 0.01); Compared with the CSDS group, the learning and memory of the CSDS + DHM group were improved significantly, the anxiety level of the mice was reduced significantly, and the immobility time in TST and FST was reduced significantly. The protein level of SIRT1 in hippocampus was increased significantly (P< 0.05 or P< 0.01). Conclusion: DHM ameliorates the cognitive impairment, anxiety like behavior and depression like behavior of mice induced by CSDS and up-regulates the expression of SIRT1 protein.

Key words: dihydromyricetin, chronic social defeat stress, cognitive dysfunction, depression, anxiety, SIRT1

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