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中国应用生理学杂志 ›› 2020, Vol. 36 ›› Issue (2): 165-170.doi: 10.12047/j.cjap.5861.2020.037

• 研究论文 • 上一篇    下一篇

抗阻训练对增龄大鼠骨骼肌线粒体功能的影响*

苏艳红1△, 袁乾坤2, 肖蓉3, 陈娟1, 李强4, 张世超1   

  1. 1.辽宁师范大学体育学院, 辽宁省运动人体科学重点实验室, 大连116029;
    2.辽宁省大连市旅顺口区大华小学, 大连116041;
    3.辽宁师范大学生命科学院, 大连116081;
    4.周口科技职业学院, 河南 周口 466000
  • 出版日期:2020-03-28 发布日期:2020-07-31
  • 通讯作者: Tel: 0411-84258545; E-mail: suyanhong2494@163.com
  • 基金资助:
    *辽宁省教育厅课题(L2014430)

Effects of resistance training on mitochondrial function in skeletal muscle of aging rats

SU Yan-hong1△, YUAN Qian-kun2, XIAO Rong3, CHEN Juan1, LI Qiang4, ZHANG Shi-chao1   

  1. 1. College of Physical Education, Provincial Key Laboratory of Sports Human Science, Liaoning Normal University, Dalian 116029;
    2. Lushunkou District Dahua Primary School, Dalian 116041;
    3. Academy of Life Sciences, Liaoning Normal University,Dalian 116081;
    4. Zhoukou Vocational College of Science and Technology, Zhoukou 466000, China
  • Online:2020-03-28 Published:2020-07-31

摘要: 目的: 从线粒体动力学的角度,探讨抗阻运动对增龄大鼠骨骼肌线粒体功能的影响。方法: 40只雄性SD大鼠随机分为4组:2月龄安静对照组(C1组)、2月龄抗阻运动训练组(R1组)、6月龄安静对照组(C2组)、6月龄抗阻运动训练组(R2组),每组10只。C1、C2组正常喂养,R1、R2组大鼠进行跑台坡度为35°,速度为15 m/min的抗阻运动,一次跑动15 s,间歇30 s,4次为一组,组间间歇3 min,3组为一次循环,一天为2个循环,循环间歇10 min,每周6 d,共8周。采用Western blot法测定各组大鼠股四头肌线粒体融合蛋白2(Mfn2)、GTP酶1(DRP1) 蛋白含量,使用流式细胞仪测定各组大鼠股四头肌线粒体膜电位(ΔΨm)、活性氧(ROS)和游离钙(Ca2+)水平。结果: ① 与C1组相比,R1组大鼠DRP1蛋白升高(P<0.01)、Mfn2蛋白无显著变化,C2组大鼠DRP1、Mfn2蛋白均降低(P均<0.01);与C2组相比,R2组大鼠DRP1、Mfn2蛋白均升高(P<0.01,P<0.05);与R1组相比,R2组DRP1、Mfn2蛋白均降低(P<0.01,P<0.05)。② 与C1组相比,R1组Ca2+含量降低(P<0.01)、C2组Ca2+含量升高(P<0.01);与C2组相比,R2组Ca2+含量降低(P<0.01);与R1组相比,R2组Ca2+含量升高(P<0.01)。③ 与C1组相比,R1组ROS含量有所上升,但无显著性差异,C2组ROS含量升高(P<0.01);与C2组相比,R2组ROS含量降低(P<0.01);与R1组相比,R2组ROS含量升高(P<0.01)。④ 与C1组相比,C2组ΔΨm降低(P<0.01);与C2组相比,R2组ΔΨm升高(P<0.01);与R1组相比,R2组ΔΨm有所降低,但无统计学差异。结论: 大鼠增龄过程中股四头肌线粒体出现Ca2+堆积、活性氧增多、线粒体膜电位下降、融合蛋白减少等现象,抗阻训练可有效改善这些变化。

关键词: 大鼠, 抗阻训练, 股四头肌, 线粒体, 融合蛋白2

Abstract: Objective: To investigate the effects of resistance exercise on mitochondrial function in skeletal muscle of aging rats. Methods: Forty male Sprague-Dawley rats were randomly assigned to 4 groups, 2-month sedentary control group (C1; n=10), 2-month with resistance training group (R1; n=10), 6-month sedentary control group (C2; n=10), 6-month with resistance training group (R2; n =10 ). Rats in R1 and R2 groups were arranged for resistance training for 8 weeks. This program consisted of interval running on a treadmill, speed 15 m·min-1, 35° incline, duration 15 s, interval 30 s, 4 times/group, 3 groups/cycle, 2 cycles per day, 6 days per week, a total of 8 weeks. The expressions of mitochondrial fusion protein 2(Mfn2) and dynamin-related protein 1(DRP1) in rat quadriceps were detected by Western blot, and the changes of mitochondrial membrane potential (ΔΨm), reactive oxygen species (ROS) and Ca2+ concentration were measured by flow cytometry. Results: ①Compared with C1 group, the expression of DRP1 protein in R1 group was increased (P<0. 01), and the Mfn2 protein in R1 group had no significant difference, both DRP1 and Mfn2 protein in C2 group were decreased (P<0. 01);compared with C2 group, the DRP1 and Mfn2 protein in R2 group were similarly increased (P<0. 01, P<0. 05);compared with R1 group, the DRP1 and Mfn2 protein in R2 group were both decreased (P<0. 01). ② Compared with C1 group, the Ca2+ content of R1 group was decreased (P<0. 01) and the Ca2+ content of C2 group was increased (P<0. 01);Compared with C2 group, the content of Ca2+ in R2 group was decreased (P<0. 01);compared with R1 group, the Ca2+ content in R2 group was increased (P<0. 01). ③ Compared with C1 group, the ROS content in R1 group was increased, but there was no significant difference, while the ROS content in C2 group was increased (P<0. 01);compared with C2 group, ROS content in R2 group was decreased (P<0. 01); compared with R1 group, the ROS content in R2 group was increased (P<0. 01). ④ Compared with group C1, the levels of ΔΨm in C2 group was decreased (P<0. 01);Compared with C2 group, The ΔΨm of R2 group was increased(P<0. 01); Compared with group R1, the ΔΨm of R2 group was decreased, but there was no statistical difference. Conclusion: During the aging process of rats, mitochondria of quadriceps femoral muscle showed Ca2+ accumulation, increased reactive oxygen species, decreased mitochondrial membrane potential, decreased fusion protein and other phenomena, and resistance training could effectively improve these changes.

Key words: rats, resistance training, quadriceps, mitochondria, fusion protein 2

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