首页  期刊介绍 征稿简则 编委会 期刊征订 广告服务 留言板 联系我们 English

中国应用生理学杂志 ›› 2020, Vol. 36 ›› Issue (2): 97-100.doi: 10.12047/j.cjap.5871.2020.022

• 研究论文 •    下一篇

当归对阴虚哮喘小鼠气道黏液高分泌及TNF-α/NF-κB信号通路的影响*

姚楠1, 韦凌霞1, 王志旺1,2△, 丁茂鹏1, 付晓艳1, 庞亚蓉1, 程小丽1, 蔺兴遥1,2, 邵晶1△   

  1. 1.甘肃中医药大学, 兰州 730000;
    2.甘肃省中药药理与毒理学重点实验室, 兰州 730000
  • 出版日期:2020-03-28 发布日期:2020-07-31
  • 通讯作者: Tel: 0931-8765395, 8765394; E-mail: wzw0933@126.com, cn221@163.com
  • 基金资助:
    *国家自然科学基金(81460668, 81660653);甘肃省自然科学基金(1606RJZA011, 1310RJZA086);甘肃省中药药理与毒理学重点实验室项目基金(ZDSYS-KJ-2018-001)

Effects of Radix Angelicae Sinensis on airway mucus hypersecretion and the TNF-α/NF-κB signaling pathway in Yin-deficiency asthmatic mice

YAO Nan1, WEI Ling-xia1, WANG Zhi-wang1,2△, DING Mao-peng1, FU Xiao-yan1, PANG Ya-rong1, CHENG Xiao-li1, LIN Xing-yao1,2, SHAO Jing1△   

  1. 1. Gansu College of Traditional Chinese Medicine, Lanzhou 730000;
    2. Key Laboratory of Pharmacology and Toxicology for Traditional Chinese Medicine of Gansu Province, Lanzhou 730000, China
  • Online:2020-03-28 Published:2020-07-31

摘要: 目的: 探讨当归对阴虚哮喘小鼠气道黏液高分泌及TNF-α/NF-κB信号通路的影响。方法: 取KM小鼠随机分为空白对照组、模型对照组、氨溴索组、当归低、中、高剂量(2、4、8 g/kg)组(n=12),采用卵蛋白与甲状腺片复制阴虚哮喘模型,观测当归对小鼠哮喘症状、IgE、TNF-α以及肺组织Muc5ac与NF-κB表达的影响。结果: 2、4、8 g/kg当归能明显缓解阴虚哮喘小鼠的哮喘症状,降低血清IgE与BALF中TNF-α水平,抑制肺组织Muc5ac与NF-κB的过度表达。结论: 当归具有明显的平喘作用,抑制TNF-α/NF-κB信号通路而缓解气道黏液高分泌是其平喘的作用机制之一。

关键词: 当归, 阴虚哮喘, 小鼠, 黏液高分泌, α-肿瘤坏死因子(TNF-α), NF-κB

Abstract: Objective: To investigate the therapeutic effects of Radix Angelicae Sinensis (RADA) on airway mucus hypersecretion and the tumor necrosis factor-α/ nuclear factor- κB (TNF-α/NF-κB) signaling pathway in Yin-deficiency asthma mice. Methods: KM mice were randomly divided into control group, model group, ambroxol group and RADA low, medium and high dose (2, 4 and 8 g/kg) group(n=12). Ovalbumin and the thyroid gland were used to replicate the model of Yin-deficiency asthma. Asthma symptoms in mice , immune globulin E (IgE) , TNF-α , and the expressions of Mucin 5ac (Muc5ac) and NF- κB in lung tissue were observed under the intervention of RADA. Results: RADA at the doses of 2,4 and 8 g/kg could alleviate the asthma symptoms of Yin-deficiency asthma mice significantly, reduce the levels of IgE in serum and TNF-α in bronchoalveolar lavage fluid (BALF), and inhibite the overexpressions of Muc5ac and NF- κB in lung tissue. Conclusion: RADA has significant anti-asthmatic effect. One of its mechanisms is to inhibit TNF-α/NF- κB signaling pathway and to alleviate airway mucus hypersecretion.

Key words: Radix Angelicae Sinensis (RADA), Yin-deficiency asthma, mouse, mucus hypersecretion, Alpha-tumor necrosis factor (TNF-α), NF-κB

中图分类号: 

版权所有 © 2015 《中国应用生理学杂志》编辑部
京ICP备16058274号-1
地址:天津市和平区大理道1号,邮编:300050  电话:022-84655184  E-mail:tjzgyish@163.com
本系统由北京玛格泰克科技发展有限公司设计开发 技术支持:support@magtech.com.cn