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中国应用生理学杂志 ›› 2019, Vol. 35 ›› Issue (5): 454-456.doi: 10.12047/j.cjap.5876.2019.098

• 研究论文 • 上一篇    下一篇

寒冷对雌性C57BL/6小鼠动情周期的影响*

赵海1,2, 李曦2+, 张莉2, 武帅2, 张永强2, 李军3, 张格祥1△, 杨丹凤2△   

  1. 1. 兰州大学公共卫生学院, 甘肃 兰州 730000;
    2. 军事科学院军事医学研究院环境医学与作业医学研究所, 天津 300050;
    3. 中国人民解放军32110部队卫生连, 黑龙江 黑河 164300
  • 收稿日期:2019-05-09 出版日期:2019-09-28 发布日期:2020-01-02
  • 通讯作者: Tel: 17627765932; E-mail: zhanggx@lzu.edu.cn, fengdyd@126.com
  • 作者简介:+:为共同第一作者
  • 基金资助:
    BWS17J025;国家自然科学基金资助(21707165)

Effects of cold exposure on estrous cycle of female C57BL/6 mice

ZHAO Hai1,2, LI Xi2+, ZHANG Li2, WU Shuai2, ZHANG Yong-qiang2, LI Jun3, ZHANG Ge-xiang1△, YANG Dan-feng2△   

  1. 1. School of Public Health, Lanzhou University, Lanzhou 730000;
    2. Institute of Environmental and Operational Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Tianjin 300050;
    3. Chinese PLA 32110, Heihe, China
  • Received:2019-05-09 Online:2019-09-28 Published:2020-01-02

摘要: 目的:研究寒冷对雌性C57BL/6小鼠动情周期的影响。方法:12只雌性小鼠随机分为对照组、低温组,每组6只;低温组每天4℃暴露4 h,每天阴道涂片法观察小鼠动情状况,对照组饲养于常温动物房;每2 d称量体重,2周后心脏取血、子宫和卵巢,检测小鼠血清E2、FSH、LH、Prl、P水平,进行子宫、卵巢的组织病理学检查。结果:与对照组比较,低温组小鼠体重无显著性差异(P>0.05),小鼠子宫脏器系数明显较低、动情间期明显延长(P<0.01),血清FSH显著升高、Prl显著降低(P<0.01),小鼠子宫腺管扩张,卵巢卵泡数量明显减少。结论:寒冷可使雌性C57BL/6小鼠动情周期延长,进而可能影响生殖功能。

关键词: 寒冷暴露, 动情周期, C57BL/6小鼠, 子宫, 卵巢

Abstract: Objective: To study the effects of cold exposure on estrous cycle of female C57BL/6 mice. Methods: Twelve healthy female C57BL/6 mice were randomly divided into two groups: control group and cold exposure group, 6 in each group. Cold exposure group was exposed to 4℃, 4 h per day, while control group stayed in normal conditions. Vaginal smears were used to observe the estrous cycle. After 2 weeks, blood and uteri were collected from each mouse after anesthetized and weighted. Serum levels of estradiol(E2), follicle-stimulating hormone(FSH), luteinizing hormone(LH), prolactin(Prl) and progesterone(P) were determined by using mouse ELISA kits. The uterus and ovary pathological slices were prepared to observe the structural changes. Results: Compared with the control group, body weight gain showed no significant differences (P>0.05). The cold group had significant lower coefficients of uterus and the diestrus phase was significantly increased after cold exposure (P<0.01). Serum level of FSH in cold group was higher and Prl was lower significantly (P<0.01). Pathological examination of uterus and ovary showed that uterine glands of cold group were expanded and the amount of follicles was decreased significantly. Conclusion: Cold exposure might increase mouse estrous cycle and affect their reproductive function.

Key words: cold exposure, estrous cycle, C57BL/6 mice, uterus, ovary

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