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中国应用生理学杂志 ›› 2020, Vol. 36 ›› Issue (1): 85-89.doi: 10.12047/j.cjap.5892.2020.019

• 研究论文 • 上一篇    下一篇

白藜芦醇对成年期追赶生长大鼠体成分的影响及机制

王素星1, 魏金刚2, 邵伟华1, 吕彩霞1, 苑晓烨1△   

  1. 1. 河北省人民医院老年病二科, 石家庄 050051;
    2. 石家庄市第五医院外科, 河北 石家庄 050000
  • 出版日期:2020-01-28 发布日期:2020-06-01
  • 基金资助:
    Tel: 0311-85988207; E-mail: 46047220@qq.com

Effect of resveratrol on body composition in adult catch-up growth rats and its mechanism

WANG Su-xing1, WEI Jin-gang2, SHAO Wei-hua1, LYU Cai-xia1, YUAN Xiao-ye1△   

  1. 1. The Second Department of Geriatrics, Hebei General Hospital, Shijiazhuang 050051;
    2. Department of General Surgery, the Fifth Hospital of Shijiazhuang City, Shijiazhuang 050000, China
  • Online:2020-01-28 Published:2020-06-01

摘要: 目的:观察白藜芦醇对成年期追赶生长大鼠体成分的影响及可能机制。方法:8周龄雄性 SD 大鼠分为6组(共2个时间点),即4周时间点3组:正常饮食4周 (NC4)组、热卡限制4周(R4)组,热卡限制同时白藜芦醇治疗(R4E)组;12周时间点3组:正常饮食12周(NC12)组,追赶生长(CUG)组,追赶生长白藜芦醇治疗(CUGE)组。每组含6只大鼠,白藜芦醇用生理盐水配制成一定浓度悬浊液,按100 mg/(kg·d)剂量予实验动物灌胃治疗。实验第4周、12周检测体重、躯干和全身的肌肉及脂肪含量、躯干与全身脂肪比例,实验第12周检测骨骼肌与附睾脂肪组织SIRT1的表达,附睾脂肪组织PPARγ的表达。结果:与NC12组相比,CUG组躯干及全身的脂肪含量、躯干与全身脂肪比例、附睾脂肪组织PPARγ的表达均明显升高(P<0.05),肌肉含量、骨骼肌与附睾脂肪组织SIRT1的表达显著降低(P<0.05或P<0.01);与CUG组相比,经白藜芦醇干预后的CUGE组全身的脂肪含量、躯干与全身脂肪比例、附睾脂肪组织PPARγ的表达均明显降低(P<0.05),肌肉含量、骨骼肌与附睾脂肪组织SIRT1的表达较CUG组显著提高(P<0.05)。结论:白藜芦醇降低成年期追赶生长大鼠体脂含量,增加肌肉含量,改善腹部脂肪堆积,其机制可能与增加骨骼肌及内脏脂肪组织SIRT1表达,抑制内脏脂肪PPARγ的表达有关。

关键词: 成年期追赶生长, 体成分, 白藜芦醇, 大鼠

Abstract: Objective: To observe the effects of resveratrol on body composition in adult catch-up growth rats and to explore the possible mechanism. Methods: Eight-week-old male SD rats were randomly divided into 6 groups: normal controls for 4 weeks (NC4) group, caloric restriction for 4 weeks (R4) group, calorie restriction meanwhile resveratrol treatment for 4 weeks (R4E) group, normal controls for 12 weeks (NC12) group, catch-up growth (CUG) group and catch-up growth meanwhile resveratrol treatment for 8 weeks (CUGE) group. At the end of the four-week and twelve-week experimental period, the body weight, muscle and fat content of trunk and whole body, the ratio of trunk to whole body fat were detected, and at the end of twelve-week experimental period, the expression of SIRT1 in skeletal muscle and epididymal adipose tissue, and the expression of PPARγ in epididymal adipose tissue were detected.Results: Compared with NC12 group, the fat content of trunk and whole body and trunk to whole body fat ratio in CUG group were increased significantly, along with the expression of PPARγ in epididymal adipose tissue was increased significantly (P<0.05), while the muscle content of trunk and whole body, the expression of SIRT1 in skeletal muscle and epididymal adipose tissue in CUG group were decreased significantly compared with NC12 group (P<0.05 or P<0.01); compared with CUG group, oral administration of resveratrol distinctly reduced the body fat content and trunk to whole body fat ratio in the CUGE groups, and the expression of PPARγ in epididymal adipose tissue of CUGE group was also significantly decreased (P<0.05). Meanwhile, the muscle content and the expression of SIRT1 in skeletal muscle and epididymal adipose tissue in CUGE group were significantly increased compared with the CUG group (P<0.05).Conclusion: Resveratrol can decrease body fat content, increase muscle content and improve abdominal fat accumulation in adult catch-up growth rats, and its mechanism may be associated with increasing SIRT1 expression in skeletal muscle and visceral adipose tissue, decreasing PPARγ expression in visceral adipose tissue.

Key words: adult catch-up growth, body composition, resveratrol, rats

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