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中国应用生理学杂志 ›› 2020, Vol. 36 ›› Issue (4): 336-339.doi: 10.12047/j.cjap.5917.2020.072

• 研究论文 • 上一篇    下一篇

环状GMP-AMP合成酶高表达对乳腺癌细胞上皮间质转化的影响*

张晓金1, 张文霞2△, 朱吉玲3, 罗文盼3   

  1. 1.江苏医药职业学院, 盐城 224004;
    2.甘肃省人民医院, 兰州 730000;
    3.绍兴文理学院, 浙江 绍兴 312000
  • 收稿日期:2019-07-31 修回日期:2020-05-15 发布日期:2020-11-09
  • 通讯作者: Tel: 18861995768; E-mail: 295867361@qq.com

Effect of cyclic GMP-AMP synthase on EMT in breast cancer cells

ZHANG Xiao-jin1, ZHANG Wen-xia2△, ZHU Ji-ling3, LUO Wen-pan3   

  1. 1. Jiangsu Vocational College of Medicine, Yancheng 224005;
    2. Gansu Provincial Hospital, Lanzhou 730000;
    3. Medical College of Shaoxing University, Shaoxing 312000, China
  • Received:2019-07-31 Revised:2020-05-15 Published:2020-11-09

摘要: 目的: 探讨环状GMP-AMP合成酶(cGAS)高表达对乳腺癌MCF7细胞发生上皮间质转化(EMT)的影响。方法: 构建稳定高表达cGAS的慢病毒载体并转染MCF7细胞;转染后细胞分别培养12 h,24 h,48 h,72 h,每组实验重复三次,采用MTT检测cGAS对MCF7细胞增殖的影响; transwell法检测高表达cGAS对MCF7细胞迁移能力的影响;蛋白免疫印迹(Western blot)法分析EMT相关蛋白E-cadherin和N-cadherin的表达情况。结果: 与对照组比较,cGAS上调后MCF7细胞增殖能力显著增强(P<0.05);细胞形态学观察显示cGAS上调后诱导MCF7细胞EMT发生,细胞形态由鹅卵石样变为梭形;transwell实验结果显示,cGAS上调导致MCF7细胞迁移能力增强(P<0.05);Western blot结果表明,cGAS上调后上皮标记蛋白E-cadherin表达下降(P<0.05),间质标记蛋白N-cadherin表达增加(P<0.05)。结论: cGAS上调可增强乳腺癌细胞的增殖和迁移能力,诱导乳腺癌细胞EMT发生。

关键词: 乳腺癌, 增殖, 迁移, 上皮-间质转化, 环状GMP-AMP合成酶

Abstract: Objective: To investigate the effects of cyclic GMP-AMP synthase (cGAS) on the proliferation, migration and epithelial to mesenchymal transition (EMT) of breast cancer cells. Methods: The cGAS lentiviral vector and control fluorescence vector were transfected into breast cancer MCF7 cells and were divided into negative group (NC) and MCF7-cGAS group. The effect of cGAS on proliferation in the MCF7 cells was detected by MTT. The effect of cGAS on cell migration was detected by Transwell assay. The expressions of EMT related proteins were analyzed by Western blot. Results: After over-expressed with cGAS, the proliferation and migration of MCF7 cells were increased (P<0.05). The expression level of the epithelial markers E-cadherin was decreased, while the expression level of the mesenchymal markers N-cadherin was increased(P<0.05). Conclusion: The over-expression of cGAS increased the proliferation and migration of breast cancer cells and induced EMT in breast cancer cells.

Key words: breast cancer, proliferation, migration, epithelial to mesenchymal transition, cyclic GMP-AMP synthase

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