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中国应用生理学杂志 ›› 2020, Vol. 36 ›› Issue (4): 346-349.doi: 10.12047/j.cjap.5922.2020.074

• 研究论文 • 上一篇    下一篇

黄芪注射液对缺血性心肌病大鼠心肌自噬及心肌重塑的影响*

邵立群1, 白婷婷1, 赵强1, 王春贵2, 宣丽颖2, 王羽2△, 赵明1△   

  1. 1.内蒙古民族大学附属医院,
    2.内蒙古民族大学医学院, 通辽 028000
  • 收稿日期:2019-08-06 修回日期:2020-05-14 发布日期:2020-11-09
  • 通讯作者: Tel: 13474859991; E-mail: langzhe73@163.com, shen348@126.com
  • 基金资助:
    *国家自然科学基金项目(81360587,81960735);内蒙古自治区自然科学基金项目(2017MS(LH)0824);内蒙古自治区高等学校“青年科技英才支持计划”(NJYT-19-B14)

Astragalus injection mediates autophagy and myocardial remodeling in rats with ischemic cardiomyopathy through calumenin

SHAO Li-qun, BAI Ting-ting1, ZHAO Qiang1, WANG Chun-gui2, XUAN Li-ying2, WANG Yu2△, ZHAO Ming1△   

  1. 1. Affiliated Hospital of Inner Mongolia University for National,
    2. Medical College of Inner Mongolia University for Nationalities, Tongliao 028000, China
  • Received:2019-08-06 Revised:2020-05-14 Published:2020-11-09

摘要: 目的: 研究黄芪注射液对缺血性心肌病大鼠心肌重塑、网腔钙结合蛋白(calumenin)及自噬影响。方法: 36只雄性SD大鼠分为正常对照组(n=12)、缺血性心肌病组(n=12)、黄芪注射液组(n=12),3组大鼠术前行心电图及心脏彩超检查后,正常对照组不做任何处理,而缺血性心肌病组和黄芪注射液组大鼠开胸结扎冠状动脉20 min后,解开结扎线行再灌注后关闭胸腔建立心肌缺血模型,黄芪注射液组术后每次注射黄芪注射液10 g/kg体重,每周注射1次,共注射4次。3组大鼠术后4周行心脏彩超检查后处死大鼠取心脏行HE染色、VG染色,观察心肌病理改变,用Western blot技术检测各组大鼠心肌细胞calumenin、LC3-I、LC3-II表达变化及LC3-I /LC3-II比值变化。结果: 与缺血性心肌病组比较,黄芪注射液组大鼠心脏彩超及心肌病理得到明显改善;同时,calumenin表达增加LC3-I /LC3-II比值表达降低(P<0.01)。结论: 黄芪注射液对缺血性心肌病大鼠心室重塑及心肌细胞自噬有明显抑制作用,该作用可能是通过calumenin所介导的。

关键词: 黄芪注射液, 缺血性心肌病, 心肌重塑, 网腔钙结合蛋白, 自噬, 大鼠

Abstract: Objective: To study the effects of astragalus injection on myocardial remodeling, calumenin and autophagy in rats with ischemic cardiomyopathy.Methods: Thirty-six male SD rats were divided into normal control group, ischemic cardiomyopathy group and astragalus injection group, 12 in each group. Electrocardiogram (ECG) and echocardiography were performed before operation in three groups. Rats in ischemic cardiomyopathy group and astragalus injection group underwent thoracotomy and ligation of coronary artery for 20 minutes, then thoracic cavity was closed after reperfusion. In the astragalus injection group,10 g/kg body weight of Astragalus injection was injected once a week, four times in total. Four weeks after operation, rats in three groups were executed by echocardiography and their hearts were collected for Hematoxylin-Eosin (HE) staining and Van Gieson (VG) staining to observe myocardial pathological changes. Calumenin, LC3-I, LC3-II expressions and LC3-I/LC3-II ratio were detected by Western blot.Results: Compared with ischemic cardiomyopathy group, the echocardiography and myocardial pathology of rats in astragalus injection group changed obviously, and the expressions of calumenin, LC3-I, LC3-II and LC3-I/LC3-II ratio changed significantly (P<0.01).Conclusion: Astragalus injection has apparent inhibitory effect on ventricular remodeling and autophagy of myocardial cells in rats with ischemic cardiomyopathy, which may be mediated by calumenin.

Key words: astragalus injection, ischemic cardiomyopathy, myocardial remodeling, calumenin, autophagy, rat

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