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中国应用生理学杂志 ›› 2020, Vol. 36 ›› Issue (3): 283-288.doi: 10.12047/j.cjap.5938.2020.062

• 技术方法 • 上一篇    

改良型大鼠鞘内穿刺置管针及导管末端注药装置在大鼠鞘内置管术中的应用研究

徐昌顺, 孙培, 林春   

  1. 福建医科大学基础医学院 脑老化与神经变性疾病重点实验室疼痛研究中心, 福州 350108
  • 收稿日期:2019-09-10 修回日期:2020-04-14 发布日期:2020-09-25
  • 通讯作者: Tel: 0591-22862439; E-mail: chunlin77550@126.com
  • 基金资助:
    国家自然科学基金面上项目(81471138);福建省自然科学基金面上项目(2018J01834);福建省科技厅科技创新联合资金项目(2018Y9069);福建省财政专项基金(2019B028)

A new design puncture needle and a device of microcatheter protection for lumbar intrathecal catheterization in rats

XU Chang-shun, SUN Pei, LIN Chun   

  1. Department of the pain Research Center, Key Laboratory of Brain Aging and Neurodegenerative Diseases, School of Basic Medical Sciences, Fujian Medical University, Fuzhou 350108, China
  • Received:2019-09-10 Revised:2020-04-14 Published:2020-09-25

摘要: 目的: 介绍一种大鼠鞘内穿刺置管针及导管末端注药装置在大鼠鞘内置管术中的应用,并评价其可行性和有效性。方法: 60只清洁级SD雄性大鼠,随机分为常规组(C组,n=30)和改良组(M组,n=30)。C组使用尖端磨平的20G针头穿刺置管,置入微量导管后于大鼠颈部两耳间穿出,留置2~3 cm,导管末端热封后游离放置;M组使用鞘内穿刺置管针进行操作,并以微量注射旋塞保护鞘内导管末端。术后1 d评估两组大鼠运动功能,分别于术后1、3、7、14、21 d进行利多卡因试验,至30 d鞘内注射亚甲蓝定位,观察并记录鞘内导管位置;记录两组鞘内给药操作时间;测定术前及术后上述时点两组大鼠机械刺激缩足反应阈值 (PWT);术前及术后一周行旷场实验,评估两组大鼠自主活动情况。结果: 运动功能分级:C组Ⅰ级75.9%,Ⅱ级20.7%,Ⅲ级3.4%,M组Ⅰ级96.7%,Ⅱ级3.3%,M组运动功能分级Ⅰ级占比率显著高于C组(P<0.05);利多卡因实验和亚甲蓝定位导管位置显示:C组分别于置管后第14日、21日各有1例脱落,至置管后第30日,共有4例导管脱落,而M组导管均在位,两组相比有统计学差异(P<0.05);M组鞘内注药操作时间多在1 min左右,而C组超过3 min,两组相比有显著统计学差异(P<0.01);PWT变化情况:两组置管后一周内PWT有波动,第3日降至最低,两组与术前相比均有显著差异(P< 0.05),置管后7 d逐渐恢复至术前水平,两组间比较无差异;旷场实验:M组置管前后在总路程、中央区活动路程以及运动时间和速度等方面均无差异,两组间各指标之间相比也无差异。结论: 改良型大鼠鞘内穿刺置管针较常规穿刺置管工具穿刺效能一致,但对大鼠运动功能影响小;微量注射旋塞能有效避免鞘内导管脱出,快速便捷完成注药,并对大鼠自主活动无影响,值得进一步推广使用。

关键词: 鞘内置管, 大鼠, 蛛网膜下腔, 疼痛, 微量注射

Abstract: Objective: To introduce a new design needle and a device of microcatheter protection for lumbar intrathecal catheterization in rats,and evaluate its feasibility and effectiveness.Methods: Sixty pathogen-free adult male Sprogue-Dawley rats were randomly divided into two groups(n=30 in each group), the control group (group C) and the modification group(group M). The traditional puncture device, 20G needle, was used in the group C without extemal shielding protection. The new design puncture needle and the microinjection cock were used in the group M. All rats were assessed for motor function on postoperative. The motor function was evaluated 1 day afteroperation. Lidocaine was injected in the catheter at 1st,3rd,7th,14th,21st day post-catheterization, methylene blue was injected in intrathecal at 30th day after operation, and the catheter location was observed. The paw withdrawal threshold(PWT) was measured at 1st,3rd,7th,14th,21st,30th day after operation, open-field test was tested at preoperative and one week postoperative for the purpose of evaluating the autonomous behavior of rats. Results: About motor function:level Ⅰ 75.9%,level Ⅱ 20.7%,level Ⅲ 3.4% in group C, and level Ⅰ 96.7%,level Ⅱ 3.3% in group M, Compared with group C,group M had higher percentage of the level Ⅰ in motor function (P<0.05);Lidocaine test and methylen blue location showed that each one case of catheter was removed on the 14th and 21st day after intubation in group C, and total four cases were removed till the 30th day, while all catheters were in normal location in group M. There was significant difference between two groups in protection of the extemal portion of catheter(P<0.05); The time of intrathecal injection in group M was only 1 minute, and it spent more than 3 minutes in group C. Compared with group C,the time of intrathecal injection is significantly shorter in group M(P<0.01);PWT was reduced to the lowest on the third day after catheterization, and there was significant difference compared with preoperative(P<0.05), PWT recovered on the 7th day and there were no significant difference between two groups; Compared with preoperative, there was no significant difference in the parameters of the group M in the open field test, neither between two groups. Conclusion: The new design puncture needle by its less injury and higher efficiency can be used in intrathecal catheterization. The microinjection cock is reliable and convenient for repeat injection with a perfect protection function of the external portion of catheter, meanwhile it has no impact on rats’ autonomous behavior so that it is worthy of further promoting.

Key words: intrathecal catheterization, rat, spinal subaraehnoid space, pain, microinjection

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