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中国应用生理学杂志 ›› 2020, Vol. 36 ›› Issue (5): 438-443.doi: 10.12047/j.cjap.5991.2020.093

• 研究论文 • 上一篇    下一篇

FABP5-PPARγ信号通路对血管性痴呆大鼠学习记忆及脂质代谢的影响*

陈丹丹1, 董莹2, 杨晓丹3△   

  1. 1.天津中医药大学第一附属医院脑血管病科, 天津 300300;
    2.吉林省肿瘤医院放疗三科, 长春 130012;
    3.中国人民解放军第九八三医院神经内科, 天津 300142
  • 收稿日期:2019-12-19 修回日期:2020-06-09 发布日期:2021-02-25
  • 通讯作者: Tel: 022-84683731; E-mail: xiaodanyang2006@126.com
  • 基金资助:
    *吉林省卫生建康委科技项目(2014zc032)

Effects of FABP5-PPARγ signaling pathway on learning-memory ability and lipid metabolism in rats with vascular dementia

CHEN Dan-dan1, DONG Ying2, YANG Xiao-dan3△   

  1. 1. Department of Cerebrovascular Diseases, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300300;
    2. Department of Radiation Ⅲ, Tumor Hospital of Jilin Province, Changchun 130012;
    3. Department of Neurology, the 983th Hospital of People's Liberation Army, Tianjin 300142, China
  • Received:2019-12-19 Revised:2020-06-09 Published:2021-02-25

摘要: 目的: 探讨脂肪酸结合蛋白5(FABP5)-过氧化物酶体增殖物激活受体γ(PPARγ)信号通路对血管性痴呆(VD)大鼠学习记忆及脂质代谢的影响及其作用机制。方法: ①采用双侧颈总动脉结扎法制备VD模型大鼠,设立正常对照组(WT组)、假手术组(sham组)及VD模型组;②设立WT组和WT+FABP5抑制剂组。4周后行Morris水迷宫实验检测大鼠空间学习记忆能力;采用RT-qPCR及Western blot方法测定脑内FABP5、PPARγ、p- PPARγ及脂蛋白脂肪酶(LPL)在转录水平和蛋白水平的表达;用试剂盒检测脑组织中总胆固醇(TC)、甘油三酯(TG)及游离脂肪酸(FFA)含量。结果: 与WT组和sham组相比,VD模型和FABP5抑制剂组大鼠学习记忆能力明显下降(P<0.05, P<0.01),脑内的FABP5、PPARγ、p- PPARγ及LPL在转录水平和蛋白水平上表达显著降低(P<0.05, P< 0.01);脑内的TC、TG和FFA含量明显提高(P<0.05, P<0.01)。结论: FABP5可通过PPARγ和LPL影响VD大鼠的学习记忆和脂质代谢。

关键词: 血管性痴呆, 脂肪酸结合蛋白5, 过氧化物酶体增殖物激活受体γ, 脂蛋白脂肪酶, 脂质代谢

Abstract: Objective: To investigate the effects of fatty acid binding protein 5(FABP5)- peroxisome proliferator-activated receptor gamma(PPARγ) signaling pathway on learning-memory ability and lipid metabolism in rats with vascular dementia(VD) and its mechanisms. Methods: ①VD model rats were established by ligating bilateral common carotid artery. These rats were divided into three groups: normal group (WT group), sham-operated group (sham group) and VD model group; ②WT group and FABP5 inhibitor group were set up. After four weeks, Morris water maze test was used to detect spatial learning and memory ability in rats. RT-qPCR and Western blot methods were used to detect the expressions of FABP5, PPARγ, p-PPARγ and lipoprotein lipase (LPL) in the brain at the mRNA and protein levels. The levels of TC, TG and FFA in the brain were detected by assay kits. Results: Compared with the WT group and sham group, the learning-memory ability of the VD model and the FABP5 inhibitor group were significantly decreased, and the expressions of FABP5, PPARγ, p-PPARγ and LPL were significantly decreased at mRNA level and protein level in the brain; and the levels of TC, TG and FFA were increased significantly in the brain. Conclusion: FABP5 can affect the learning-memory ability and lipid metabolism in VD rats through PPARγ and LPL.

Key words: Vascular dementia, fatty acid binding protein 5, peroxisome proliferator-activated receptor gamma, lipoprotein lipase, lipid metabolism

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