首页  期刊介绍 征稿简则 编委会 期刊征订 广告服务 留言板 联系我们 English

中国应用生理学杂志 ›› 2020, Vol. 36 ›› Issue (5): 481-485.doi: 10.12047/j.cjap.6004.2020.102

• 研究论文 • 上一篇    下一篇

有氧运动对高糖高脂膳食大鼠腓肠肌Nrf2-SOD的影响*

刘玉倩1,2, 张静3, 高丽娜3, 王海涛1,2△   

  1. 1.岭南师范学院体育科学学院,
    2.运动与健康研究所, 广东 湛江 524048;
    3.河北师范大学体育学院, 河北 石家庄 050024
  • 收稿日期:2020-01-08 修回日期:2020-07-09 发布日期:2021-02-25
  • 通讯作者: Tel: 17875191037; E-mail: haitaoyq@126.com
  • 基金资助:
    *河北省高等学校自然科学重点项目(ZD2017048);岭南师范学院人才专项(ZL2008,ZL2009)

Effects of aerobic exercise on Nrf2-SOD pathway in the gastrocnemius of rats with high-glucose and high-fat diet

LIU Yu-qian1,2, ZHANG Jing3, GAO Li-na3, WANG Hai-tao1,2△   

  1. 1. School of Sport and Exercise Science, Lingnan Normal University, Zhanjiang 524048;
    2. In stitute of Exercise and Health, Lingnan Normal University, Zhanjiang 524048;
    3. Department of Physical Education, Hebei Normal University, Shijiazhuang 050024, China
  • Received:2020-01-08 Revised:2020-07-09 Published:2021-02-25

摘要: 目的: 探讨有氧运动预防大鼠胰岛素抵抗中Nrf2及SOD的变化。方法: 24只12月龄SD大鼠随机分为对照组(C)、高糖高脂IR组(IR)和高糖高脂IR并运动组(IRE)。IRE进行递增负荷跑台运动,运动6周。检测腓肠肌T-SOD、CAT、MDA、GSH/GSSG,ELISA法检测腓肠肌8-OHdG含量,Western blot检测腓肠肌Nrf2和GLUT4表达。结果: ①IRE组HOMA-IR明显低于IR组(P<0.05);IRE组肌糖原明显高于IR组(P<0.01);②IRE组T-SOD和CAT、GSH/GSSG明显高于IR组(P<0.01);IRE组8-OHdG和MDA明显低于IR组(P<0.01);③IRE组腓肠肌Nrf2和GLUT4明显高于IR组 (P<0.01) 。结论: 有氧运动可激活大鼠腓肠肌Nrf2-SOD通路,提高抗氧化酶活性和糖摄取能力,预防IR发生。

关键词: 核因子E2相关因子2, 有氧运动, 腓肠肌, 胰岛素抵抗, 大鼠

Abstract: Objective: To investigate the changes of nuclear factor erythroid 2-related factor 2 (Nrf2) and superoxide dismutase (SOD) in the prevention of insulin resistance in skeletal muscle of rats by aerobic exercise. Methods: Twenty-four 12-month old Sprague-Dawley (SD) rats were randomly divided into control (C), insulin resistance model (IR) and insulin resistance model and exercise (IRE) groups. IR was induced by high- glucose and high-fat diet. Rats of IRE received the increasing load running on the treadmill for 6 W. The activity of total superoxide dismutase (T-SOD), catalase (CAT), Malonaldehyde (MDA) and glutathione / L-glutathione oxidized (GSH/GSSG) were measured by experiment kits. The content of 8-hydroxy-2 deoxyguanosine (8-OHdG) was measured by ELISA Kits. The expressions of Nrf2 and Glucose transporters 4 (GLUT4) in gastrocnemius were detected by Western Blot. Results: ①Compared with IR, the content of HOMA-IR was decreased in IRE (P<0.05), while the content of muscle glycogen was increased in IRE (P<0.01). ② The activities of T-SOD, CAT, and GSH/GSSG in IRE were significantly increased compared with those in IR (P<0.01). Compared with IR, the contents of 8-OHdG and MDA in IRE were significantly decreased (P<0.01). ③ The expressions of Nrf2 and GLUT4 in gastrocnemius of IRE rats were increased than those in IR (P<0.01). Conclusion: The Nrf2 pathway was activated in gastrocnemius of rats after aerobic exercise, which promoted the activity of antioxidant enzymes, and prevented IR induced by high- glucose and high-fat diet.

Key words: Nrf2, aerobic exercise, gastrocnemius, insulin resistance, rat

中图分类号: 

版权所有 © 2015 《中国应用生理学杂志》编辑部
京ICP备16058274号-1
地址:天津市和平区大理道1号,邮编:300050  电话:022-84655184  E-mail:tjzgyish@163.com
本系统由北京玛格泰克科技发展有限公司设计开发 技术支持:support@magtech.com.cn