首页  期刊介绍 征稿简则 编委会 期刊征订 广告服务 留言板 联系我们 English

中国应用生理学杂志 ›› 2021, Vol. 37 ›› Issue (3): 287-292.doi: 10.12047/j.cjap.6009.2021.005

• 研究论文 • 上一篇    下一篇

不同强度有氧运动对大鼠房室结形态结构及VEGF、VIP、Syn表达的影响

王友华, 陈伟, 马美, 戴高远, 滑艺杰   

  1. 陕西师范大学 运动生物学研究所, 西安 710119
  • 出版日期:2021-05-28 发布日期:2021-08-09
  • 通讯作者: Tel: 17782563881; E-mail: wangyouh@snnu.edu.cn
  • 基金资助:
    *国家自然科学基金资助项目(81570449);中央高校基本科研业务费专项资金资助项目(GK201803097);陕西省自然科学基础研究计划(2019JM-301);大学生创新创业训练计划项目(S202010718050)。

Effects of different intensity exercise on morphology and structure of atrioventricular node and protein expressions of VEGF, VIP and Syn in rats

WANG You-hua, CHEN Wei, MA Mei, DAI Gao-yuan, HUA Yi-jie   

  1. Institute of Sports and Exercise Biology, Shaanxi Normal University, Xi'an 710119, China
  • Online:2021-05-28 Published:2021-08-09

摘要: 目的: 探讨不同强度有氧运动对大鼠房室结形态结构和心肌血管内皮生长因子(VEGF)、血管活性肠肽(VIP)、突触素(Syn)表达的影响。方法: 3月龄SD大鼠随机分为3组:安静对照组(C),中等强度有氧运动组(AE),大强度有氧运动组(FE),每组8只,采用8周跑台训练方式建立大鼠中等强度有氧运动和大强度有氧运动模型。结果: 与安静对照组相比,中等强度有氧运动组房室结肌纤维分布均匀、间隙较小,有透明感且胶原紧紧包绕房室结,闰盘结构清晰,连接完整,毛细血管管壁较厚弹力纤维较发达,房室结VEGF、VIP的表达显著升高(P< 0.05),Syn表达无显著性变化;大强度有氧运动组房室结肌纤维分布不均、排列异常、细胞之间连接紊乱、出现空泡化现象,闰盘发生扭曲,盘旋重叠,模糊、不连续并有极度扩张现象,毛细血管管壁较厚且内皮细胞排列稀疏凌乱,而且,大强度有氧运动显著抑制VEGF、VIP、Syn的表达(P<0.05)。结论: 不同强度有氧运动训练对房室结形态结构和VEGF、VIP、Syn的表达均有明显的影响,中等强度有氧运动可以维持并促进房室结正常形态结构,而大强度有氧运动对房室结的形态与结构产生较大的损伤,推测与大强度运动诱导心律失常关系密切。

关键词: 不同强度有氧运动, 房室结, 形态结构, VEGF, VIP, Syn, 大鼠

Abstract: Objective: To investigate the effects of different intensities of aerobic exercise on the morphology of the atrioventricular node and the expressions of vascular endothelial growth factor (VEGF), vasoactive intestinal peptide (VIP) and synaptophysin (Syn) in rats. Methods: Three-month-old SD rats were randomly divided into 3 groups: quiet control group (C), moderate-intensity aerobic exercise group (AE), and high-intensity aerobic exercise group (FE), each with 8 rats. The moderate-intensity aerobic exercise and high-intensity aerobic exercise rat models were rats established by using 8-week treadmill training ways. Results: Compared with the quiet control group, in the moderate-intensity aerobic exercise group, the atrioventricular nodule muscle fibers were evenly distributed, with smaller gaps, a sense of transparency, and collagen tightly wraps around the atrioventricular nodules. The intercalary disc had a clear structure and complete connection, and the capillary tube wall was relatively clear. Thick elastic fibers were more developed, the expression levels of VEGF and VIP in the atrioventricular node were increased significantly (P<0.05), and there was no significant change in the expression of Syn. In the high-intensity aerobic exercise group, the atrioventricular nodule muscle fibers were unevenly distributed and arranged abnormally, connection between cells were disordered, and vacuolated. The intercalary disk was twisted, circling and overlapping, fuzzy, discontinuous, and extremely dilated. The wall was thick and the endothelial cells were arranged sparsely and disorderly. Moreover, high-intensity aerobic exercise significantly inhibited the expressions of VEGF, VIP, and Syn (P<0.05). Conclusion: Aerobic exercise training of different intensities has obvious effects on the morphology and structure of the atrioventricular node and the expressions of VEGF, VIP and Syn. Moderate aerobic exercise can maintain and promote the normal morphology and structure of the atrioventricular node, while high-intensity aerobic exercise can damage the morphology and structure of the ventricular node, which is presumably closely related to the arrhythmia induced by high-intensity exercise.

Key words: aerobic exercise of different intensities, atrioventricular node, morphology, VEGF, VIP, Syn, rat

中图分类号: 

版权所有 © 2015 《中国应用生理学杂志》编辑部
京ICP备16058274号-1
地址:天津市和平区大理道1号,邮编:300050  电话:022-84655184  E-mail:tjzgyish@163.com
本系统由北京玛格泰克科技发展有限公司设计开发 技术支持:support@magtech.com.cn