党参总皂苷对TNBS诱导的大鼠溃疡性结肠炎的保护作用及其机制

doi:10.12047/j.cjap.6051.2021.032

中国应用生理学杂志 ›› 2021, Vol. 37 ›› Issue (4): 397-401.doi: 10.12047/j.cjap.6051.2021.032

党参总皂苷对TNBS诱导的大鼠溃疡性结肠炎的保护作用及其机制

刘雪枫^1,2, 乔婧¹, 高建德^1,3, 陈正君², 刘雄^1△

1. 甘肃中医药大学药学院, 兰州 730000;
2. 甘肃省中藏药化学与质量研究省级重点实验室, 兰州 730000;
3. 甘肃省中药质量与标准研究重点实验室, 兰州 730000

出版日期:2021-07-28 发布日期:2021-08-09
通讯作者: ^△Tel: 13919798335; E-mail: lx@gszy.edu.cn
基金资助:
^*兰州市科技局项目(2014-1-17);甘肃省中药质量与标准研究重点实验室培育基地开放基金项目(ZYZL16-012);甘肃省中藏药化学与质量研究省级重点实验室开放基金(ZZY-2018-04)

Protective effects of total saponins of Codonopsis on ulcerative colitis induced by TNBS in rats and its mechanism

LIU Xue-feng^1,2, QIAO Jing¹, GAO Jian-de^1,3, CHEN Zheng-jun², LIU Xiong^1△

1. Pharmacy Collage of Gansu University of Chinese Medicine, Lanzhou 730000;
2. Key Laboratory of Chinese and Tibetan Medicine Chemistry and Quality Research in Gansu Province, Lanzhou 730000;
3. Key Laboratory of Traditional Chinese Medicine Quality and Standard in Gansu Province, Lanzhou 730000, China

Online:2021-07-28 Published:2021-08-09

摘要/Abstract

摘要： 目的: 探讨党参总皂苷(TSC)对大鼠实验性溃疡性结肠炎(UC)的治疗作用及其作用机制。方法: 50只雄性Wistar大鼠随机分为5组:对照组、模型组、柳氮磺胺嘧啶(SASP)阳性对照组(0.3 g/kg)、TSC高剂量组(1.2 g/kg)、TSC低剂量组(0.4 g/kg),用三硝基苯磺酸(TNBS)/乙醇联合灌肠制作大鼠UC模型,给药21 d后,通过观察大鼠症状和体征、疾病活动指数(DAI)、结肠粘膜损伤指数(CMDI)、结肠组织形态;测定结肠组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、炎症因子白介素-6(IL-6)、白介素-10(IL-10)、肿瘤坏死因子α(TNF-α)的含量;检测结肠组织中细胞核内核转录因子-κB(NF-κB)蛋白表达;最终评价TSC的治疗效果。结果: 与对照组比较,模型组大鼠DAI、CMDI评分显著升高,结肠粘膜损伤严重,说明造模成功。与模型组比较,TSC高低剂量组均能显著降低UC大鼠DAI评分、CMDI评分(P＜0.05);改善结肠黏膜形态;升高结肠组织中SOD活力,降低MDA含量(P＜0.05),抑制结肠组织中IL-6、TNF-α mRNA水平,促进IL-10 mRNA表达(P＜0.01);同时降低结肠中NF-κB蛋白表达(P＜ 0.01),且TSC高剂量组优于低剂量组(P＜0.05)。结论: TSC对UC大鼠结肠黏膜损伤具有显著保护作用,以高剂量组为佳;其机制可能与抗脂质过氧化,抑制NF-κB信号通路从而调控炎性因子的释放有关。

关键词: 党参总皂苷, 溃疡性结肠炎, NF-κB, 细胞因子, 大鼠

Abstract: Objective: To study the protective effects and mechanisms of total saponins of Codonopsis (TSC) on ulcerative colitis in rats. Methods: Fifty male Wistar rats were randomly divided into 5 groups: control group, model group, salazosulfadiazine (SASP) positive control group (0.3 g/kg), TSC high- and low-dose experimental groups(1.2, 0.4 g/kg). UC rat model was established by trinitrobenzene sulfonic acid (TNBS)/ ethanol enema. After administration for 21 days, the rats' symptoms and signs, disease activity index (DAI), colonic mucosal injury index (CMDI) and colonic tissue morphology were observed. The contents of superoxide dismutase (SOD), malondialdehyde (MDA), inflammatory cytokines interleukin-6 (IL-6), interleukin-10 (IL-10) and tumor necrosis factor (TNF-α) in colon tissues were determined. Protein expression of nuclear nuclear transcription factor-κB (NF-κB) in colon tissues was detected. Finally, the effect of TCS therapy was evaluated. Results: Compared with the control group, the DAI and CMDI scores of the rats in the model group were increased significantly, meanwhile the colonic mucosa was seriously damaged, indicating that the model was successful. Compared with the model group, the TSC high and low dose groups could significantly reduce the DAI and CMDI score (P＜0.05) and improve the colonic mucosa form. TSC also could increase the SOD activity and decrease MDA content in colon tissues(P＜0.05), while inhibit the levels of IL-6 and TNF-α mRNA in the colon tissues and promote the expression of IL-10 mRNA (P＜0.01). At the same time, TSC reduced the expressions of NF-κB protein in the colon (P＜0.01). The TSC high-dose group was superior to the low-dose group (P＜0.05). Conclusion: TSC has significant protective effects on ulcerative colonic mucosal damage in UC rats, and there is a dose-dependent relationship; its mechanism may be related to anti-lipid peroxidation and inhibiting the NF-κB signaling pathway to regulate the release of inflammatory factors.

Key words: total saponins of codonopsis, ulcerative colitis, NF-κB, cytokine, rat

中图分类号:

R285.5

刘雪枫, 乔婧, 高建德, 陈正君, 刘雄. 党参总皂苷对TNBS诱导的大鼠溃疡性结肠炎的保护作用及其机制[J]. 中国应用生理学杂志, 2021, 37(4): 397-401.

LIU Xue-feng, QIAO Jing, GAO Jian-de, CHEN Zheng-jun, LIU Xiong. Protective effects of total saponins of Codonopsis on ulcerative colitis induced by TNBS in rats and its mechanism[J]. CJAP, 2021, 37(4): 397-401.

参考文献 16

[1]	Ungaro R, Mehandru S, Allen PB, et al. Ulcerative colitis[J]. The Lancet, 2017, 389(10080): 1756-1770.
[2]	Conrad K, Roggenbuck D, Laass MW, et al. Diagnosis and classification of ulcerative colitis[J]. Autoimun Rev, 2014, 13(4/5): 463-466.
[3]	肖迅. 中药有效成分/有效部位治疗溃疡性结肠炎的研究进展[J]. 中药药理与临床, 2017, 33(3): 219-223.
[4]	国家药典委员会编. 中国药典一部[S]. 北京: 化学工业出版社, 2015: 281-282.
[5]	张声生. 溃疡性结肠炎中医诊疗专家共识意见(2017)[J]. 中华中医药杂志, 2017, 32(8): 3585-3589.
[6]	刘美霞, 戚进, 余伯阳. 党参药理作用研究进展[J]. 海峡药学, 2018, 30(11): 36-39.
[7]	乔婧, 薛晶晶, 高建德, 等. 星点设计- 响应面法优化党参总皂苷提取工艺[J]. 中兽医医药杂志, 2019, 38(3): 36-42.
[8]	王倩, 别玉龙, 王豆, 等. 蒲公英多糖对溃疡性结肠炎大鼠IL-6/STAT3信号通路的影响[J]. 中国应用生理学杂志, 2017, 33(5): 422-425.
[9]	Walter EC, Chaitanya VG, Mihir P, et al. VEGF-A isoform modulation in an preclinical TNBS model of ulcerative colitis: protective effects of a VEGF164b therapy[J]. J Transl Med, 2013, 207(11): 2-12.
[10]	Luk HH, Ko K, Fung HS, et al. Delineation of the protective action of zinc sulfate on ulcerative colitis in rats[J]. Eur J Pharmacol, 2002, 443(1-3): 197-204.
[11]	裴银奇, 赵党生. 基于中医传承辅助系统的溃疡性结肠炎方剂用药规律分析[J]. 亚太传统医药, 2016, 12(24): 92-94.
[12]	范文涛, 王攀红, 王倩. 马齿苋多糖对溃疡性结肠炎大鼠肠组织IL-6 /STAT3及NF-κB的影响[J]. 中国应用生理学杂志, 2018, 34(3): 263-267.
[13]	崔宇, 李晓栩, 黄缄. 三七和银杏叶片对高原脱习服大鼠心功能及血清炎症因子的影响及其机制[J]. 中国应用生理学杂志, 2019, 35(1): 34-38.
[14]	Sun LD, Wang F, Dai F, et al. Development and mechanism investigation of a new piperlongumine derivative as a potent anti-inflammatory agent[J]. Biochem Pharmacol, 2015, 95(3): 156-169.
[15]	Schinzari F, Armuzzi A, Pascalis BD, et al. Tumor necrosisfactor-α antagonism improves endothelial dysfunction inpatients with Crohn's Disease[J]. Clin Pharmacol Ther, 2008, 83(1): 70-76.
[16]	Yukitake H, Takizawa M, Kimura H. Macrophage migration inhibitory factor as an emerging drug target to regulate antioxidant response element system[J]. Oxid Med Cell Longev, 2017, 2017: 1-6.

党参总皂苷对TNBS诱导的大鼠溃疡性结肠炎的保护作用及其机制

Protective effects of total saponins of Codonopsis on ulcerative colitis induced by TNBS in rats and its mechanism

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献 16

相关文章 15

编辑推荐

Metrics

本文评价

[1]	张瑜, 路青华, 曹海芳, 张生荣, 王虎德. GPR81激动剂对非酒精性脂肪肝病大鼠胰岛素抵抗的影响[J]. 中国应用生理学杂志, 2021, 37(4): 354-358.
[2]	丁海丽, 黄增浩, 任在方, 孙竹昕, 李俊平, 王瑞元. 针刺对大鼠运动性骨骼肌损伤内质网应激的干预作用及机制[J]. 中国应用生理学杂志, 2021, 37(4): 359-364.
[3]	杨向君, 王羽, E.巴雅日玛, 赵明, S.贺希格扎日嘎拉. 蒙药绍沙-7味丸防治大鼠心肌缺血/再灌注损伤的作用[J]. 中国应用生理学杂志, 2021, 37(4): 380-384.
[4]	郝卯林, 楼国强, 刘秀洁, 钱巍, 王嘉, 周卓琳, 王万铁. 细胞自噬在大鼠缺血/再灌注肺损伤中的调控作用[J]. 中国应用生理学杂志, 2021, 37(4): 385-388.
[5]	耿元文, 林琴琴, 王湘怡, 李若明, 田振军. 间歇运动对心梗大鼠心肌氧化应激和炎症的影响及其机制[J]. 中国应用生理学杂志, 2021, 37(4): 439-444.
[6]	徐云, 陈雪辉, 白立炜, 尹清风, 冯春瑜, 张清贵. 达格列净对2型糖尿病大鼠肾脏葡萄糖转运蛋白2及葡萄糖转运蛋白4基因表达的影响^*[J]. 中国应用生理学杂志, 2020, 36(6): 565-570.
[7]	王丹, 高峰, 陈慧, 项伟玲, 骆志显, 金丽琴. 人参归脾丸对脾不统血证大鼠肝损伤的保护作用及其机制^*[J]. 中国应用生理学杂志, 2020, 36(6): 571-575.
[8]	韦凌霞, 丁茂鹏, 王志旺, 刘雪枫, 庞亚蓉, 郭玫. 鳖甲育肝颗粒对乙醇性肝纤维化大鼠的影响^*[J]. 中国应用生理学杂志, 2020, 36(6): 582-586.
[9]	张冉, 马梦尧, 苏欣宇, 孟想, 姜鲲鹏, 李曙, 洪云. 大鼠脑缺血/再灌注过程中血流量和脑组织含水量的变化趋势^*[J]. 中国应用生理学杂志, 2020, 36(6): 586-589.
[10]	苏海容, 程文姚, 袁秋虹, 欧阳菁, 邓伟民. 补肾壮骨颗粒对去卵巢大鼠血清GH和IGF-1及其骨组织中受体表达的影响^*[J]. 中国应用生理学杂志, 2020, 36(6): 605-610.
[11]	金艳, 刘文明. 多配体蛋白多糖-1在慢性阻塞性肺疾病大鼠肺上皮间质转化中的作用及机制^*[J]. 中国应用生理学杂志, 2020, 36(6): 611-615.
[12]	冉卉, 尹浩, 刘窗溪, 韩国强, 高方友, 谌鸿斌, 付航, 徐晓钟, 黎涛, 马骏. 甲状腺素对动脉瘤性蛛网膜下腔出血大鼠脑缺氧诱导因子-1α表达的影响及其机制^*[J]. 中国应用生理学杂志, 2020, 36(6): 648-652.
[13]	李刚, 籍胤玺, 高毅, 冯彦冰. 帕瑞昔布对骨关节炎大鼠软骨细胞的影响^*[J]. 中国应用生理学杂志, 2020, 36(6): 652-655.
[14]	孙科, 罗招亮, 胡琛, 龚廷亮, 唐国华, 吴绍萍. 小檗碱对大鼠脑缺血/再灌注损伤的保护作用及免疫机制^*[J]. 中国应用生理学杂志, 2020, 36(6): 656-661.
[15]	潘文强, 王书凡, 丁伯平, 黄帧桧. 格列齐特对糖尿病大鼠心肌的保护作用及其机制^*[J]. 中国应用生理学杂志, 2020, 36(5): 402-407.