谷氨酰胺对大鼠运动性疲劳的改善作用及其机制

doi:10.12047/j.cjap.6060.2021.003

摘要/Abstract

摘要： 目的: 探讨谷氨酰胺(Gln)对大鼠运动性疲劳、骨骼肌氧化应激和肝脏细胞凋亡的改善作用。方法: 将8周龄SPF级雄性SD大鼠20只, 体重180～220 g,适应性喂养1周后随机分为对照组和谷氨酰胺干预组,每组10只。谷氨酰胺干预组采用每天1.0 g/kg/d谷氨酰胺灌胃(约2 ml),对照组以等体积生理盐水灌胃(约2 ml),持续7 d。随后进行力竭实验,禁食不禁水12 h后处死大鼠,检测血清及骨骼肌谷胱甘肽(GSH)和丙二醛(MDA)水平及超氧化物歧化酶(SOD)活力,检测血清乳酸(LD)水平及肌酸激酶(CK)和乳酸脱氢酶(LDH)活力;荧光实时定量PCR检测肝组织Bcl-2和Bax 基因表达水平。结果: 与对照组相比,谷氨酰胺干预组大鼠力竭时间显著延长(P＜0.05),血清CK、LDH及LD水平显著降低(P＜0.05),血清与骨骼肌中GSH和SOD水平显著提高(P＜0.05),MDA水平明显降低(P＜0.05);肝组织Bax 基因表达水平显著降低(P＜0.05),Bcl-2 基因表达水平明显显著增高(P＜ 0.05)。结论: 谷氨酰胺有缓解大鼠运动性疲劳的作用,其机制可能与降低骨骼肌氧化应激程度和延缓肝脏细胞凋亡率有关。

关键词: 谷氨酰胺, 运动性疲劳, 氧化应激, 细胞凋亡, 大鼠

Abstract: Objective: To investigate the effects of glutamine on exercise-induced fatigue, skeletal muscle oxidation and liver cell apoptosis in rats. Methods: Twenty SPF grade SD rats aged at 8 week and weight from 180 to 220 g, were divided into control group and glutamine-treated group after one week of feeding, 10 rats in each group. The rats in the glutamine group were treated with glutamine at the dose of 1.0 g/(kg·d)by intragastric administration, and the rats in control group were administrated with equal volume of normal saline. After 7 days, the exhaustion test was conducted, the content of glutathione (GSH) was detected by high-performance liquid chromatography. Superoxide dismutase (SOD), malondialdehyde (MDA), lactic acid (2-hydroxypropanoic acid (LD) were detected by enzyme-linked immunosorbent assay, and the creatine kinase(CK) was detected by electroluminescence. Activities of CK, Lactate dehydrogenase (LDH), and expression levels of Bcl-2 and Bax mRNA were detected by fluorescence quantitative RT-PCR. Results: The duration of exhaustion in the glutamine group was greater than that in the control group (P＜0.05). The serum glutathione level in the glutamine group was lower than that in the control group (P＜0.05). After exhaustion, the levels of GSH, SOD and MDA in serum and skeletal muscle of the glutamine group were higher than those of the control group significantly differences (P＜ 0.05). The serum Bax mRNA level in the glutamine group was lower than that in the control group significantly (P＜0.05). The serum bcl-2 mRNA level in the glutamine group was higher than that in the control group significantly (P＜0.05). Conclusion: Glutamine can effectively alleviate exercise-induced fatigue in rats, reduce the oxidation degree of skeletal muscle, and decrease the apoptosis rate of liver cells.

Key words: glutamine, exercise-induced fatigue, superoxide dismutase, apoptosis, rats

中图分类号:

R363.2

江涛, 韦雪亮, 肖书奇. 谷氨酰胺对大鼠运动性疲劳的改善作用及其机制[J]. 中国应用生理学杂志, 2021, 37(3): 293-296.

JIANG Tao, WEI Xue-liang, XIAO Shu-qi. Effects of glutamine on exercise-induced fatigue, skeletal muscle oxidation and liver cell apoptosis in rats[J]. CJAP, 2021, 37(3): 293-296.

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