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中国应用生理学杂志 ›› 2021, Vol. 37 ›› Issue (4): 433-438.doi: 10.12047/j.cjap.6064.2021.034

• 研究论文 • 上一篇    下一篇

虾青素复合有氧运动对D-半乳糖诱导大鼠肾脏衰老的干预作用及其机制

牛衍龙1,2, 曹建民3, 周绮云4,5, 胡戈6, 郭娴3, 刘健4, 郝敏4, 张涛3, 周海涛4,5△   

  1. 1. 赣南医学院, 江西 赣州 341000;
    2. 赣州市康复医学重点实验室, 江西 赣州 341000;
    3. 北京体育大学, 北京 100084;
    4. 北京联合大学, 北京 100101;
    5. 北京联合大学 生物活性物质与功能食品北京市重点实验室, 北京 100191;
    6. 常州大学, 江苏 常州 213164
  • 出版日期:2021-07-28 发布日期:2021-08-09
  • 通讯作者: Tel: 13611383040; E-mail: zsettle@sina.com
  • 基金资助:
    *江西省教育厅科学技术项目(GJJ190800);江西省中医药科研课题(2019A073);赣南医学院博士启动金课题(QD201817);北京市朝阳区协同创新项目(CYXC1817);北京联合大学科研项目(ZK70202005,ZK30202006);北京市高等学校高水平人才交叉培养“实培计划”项目;北京联合大学“启明星”大学生科技创新创业项目(201911417031,202011417SJ128)

Effects of astaxanthin combined with aerobic exercise on renal aging of rat induced by D-galactose and its mechanism

NIU Yan-long1,2, CAO Jian-min3, ZHOU Qi-yun4,5, HU Ge6, GUO Xian3, LIU Jian4, HAO Min4, ZHANG Tao3, ZHOU Hai-tao4,5△   

  1. 1. Gannan Medical University, Jiangxi Ganzhou 341000, China;
    2. Key Laboratory of Rehabilitation Medicine in Ganzhou, Jiangxi Ganzhou 341000, China;
    3. Beijing Sport University, Beijing 100084;
    4. Beijing Union University, Beijing 100101;
    5. Key Laboratory of Bioactive Substances and Functional Foods, Beijing Union University, Beijing 100191;
    6. Changzhou University, Changzhou 213164, China
  • Online:2021-07-28 Published:2021-08-09

摘要: 目的: 研究虾青素复合有氧运动对D-半乳糖诱导大鼠肾脏衰老的干预作用及其机制。方法: 60只3月龄SPF级SD大鼠采用两因素两水平2×2析因设计随机分为空白对照组(C组)、急性衰老组(S组)、虾青素+急性衰老组(AS组)、有氧运动+急性衰老组(ES组)、虾青素+有氧运动+急性衰老组(AES组),每组12只。大鼠腹腔注射100 mg/(kg·d) D-半乳糖复制经典急性衰老模型,并分别以20 mg/(kg·d) 虾青素和/或强度为60%最大摄氧量的有氧运动进行干预,实验周期6周。末次训练12 h后取肾脏,光镜/电镜观察肾脏组织形态/超微结构,酶联免疫吸附法检测肾脏组织超氧化物歧化酶(SOD)、γ-谷氨酸半胱氨酸合酶(γ-GCS)活性及丙二醛(MDA)含量,荧光比色法检测肾脏组织脂褐质(LDF)含量,免疫组化法检测肾脏组织核因子E2相关因子2(Nrf2)通路蛋白质表达水平。结果: 与AS、ES组比较:AES组肾脏组织形态/超微结构改善更为显著;LDF含量均显著降低(P<0.01);SOD活性均显著升高(P<0.01);γ-GCS活性显著高于AS组,而与ES组无显著性差异(P>0.05);MDA含量组间无显著性差异(P>0.05);Nrf2、磷酸化核因子E2相关因子2(p-Nrf2)蛋白质表达均显著升高(P<0.05,P<0.01);抗氧化酶血红素氧合酶1(HO-1)蛋白质表达显著高于ES组(P<0.05),而与AS组无显著性差异(P<0.05)。结论: 虾青素复合有氧运动可以延缓肾脏衰老,其作用机制可能为调控Nrf2信号通路相关蛋白质表达及下游Ⅱ相解毒酶和抗氧化酶活性,改善D-半乳糖致衰大鼠肾脏氧化应激。

关键词: 虾青素, 有氧运动, 衰老, 氧化应激, Nrf2,

Abstract: Objective: To study the effects and mechanisms of astaxanthin combined with aerobic exercise on renal senescence of rat induced by D-galactose. Methods: Sixty 3-month-old SPF SD rats were divided into control group (C group), acute senescence group (S group), astaxanthin+acute senescence group (AS group), aerobic exercise+acute senescence group (ES group), astaxanthin+aerobic exercise+acute senescence group (AES group), by two-factor two-level 2×2 factorial design with 12 rats in each group. Acute senescence model of rat was establshed by intraperitoneal injection with 100 mg/(kg·d) D-galactose, and the intervention was conducted with 20 mg/(kg·d) astaxanthin and/or aerobic exercise with 60% VO2max for 6 weeks. The histopathological/ultrastructural changes of the kidney were observed by light microscope/electron microscope; the levels of SOD, γ-GCS and MDA were detected by ELISA, and LDF in kidney was determined by fluorescence colorimetry; the protein expression of Nrf2 signaling pathway was detected by immunohistochemistry. Results: Compared with AS and ES group, in AES group, the improvement of renal tissue morphology/ultrastructure was more significant; LDF was decreased significantly (P<0.01); SOD activity was significantly increased (P<0.01); γ-GCS was significantly higher than that of AS group, but not significantly different from that of ES group (P>0.05); there was no significant difference in MDA between groups (P>0.05); the levels of Nrf2 and p-Nrf2 were increased significantly (P<0.05, P<0.01); HO-1 was significantly higher than that of ES group(P<0.05), but not significantly different compared with that of AS group(P>0.05). Conclusion: Astaxanthin combined with aerobic exercise can delay aging process of kidney, its mechanism may be that the combination regulate the protein expression in Nrf2 signaling pathway, Ⅱ detoxifying enzymes and antioxidant enzyme activity, and improve oxidative stress in kidney of rat induced by D-galactose.

Key words: astaxanthin, aerobic exercise, aging, oxidative stress, Nrf2, kidney

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