虾青素复合有氧运动对D-半乳糖诱导大鼠肾脏衰老的干预作用及其机制

doi:10.12047/j.cjap.6064.2021.034

中国应用生理学杂志 ›› 2021, Vol. 37 ›› Issue (4): 433-438.doi: 10.12047/j.cjap.6064.2021.034

虾青素复合有氧运动对D-半乳糖诱导大鼠肾脏衰老的干预作用及其机制

牛衍龙^1,2, 曹建民³, 周绮云^4,5, 胡戈⁶, 郭娴³, 刘健⁴, 郝敏⁴, 张涛³, 周海涛^4,5△

1. 赣南医学院, 江西赣州 341000;
2. 赣州市康复医学重点实验室, 江西赣州 341000;
3. 北京体育大学, 北京 100084;
4. 北京联合大学, 北京 100101;
5. 北京联合大学生物活性物质与功能食品北京市重点实验室, 北京 100191;
6. 常州大学, 江苏常州 213164

出版日期:2021-07-28 发布日期:2021-08-09
通讯作者: ^△ Tel: 13611383040; E-mail: zsettle@sina.com
基金资助:
^*江西省教育厅科学技术项目(GJJ190800);江西省中医药科研课题(2019A073);赣南医学院博士启动金课题(QD201817);北京市朝阳区协同创新项目(CYXC1817);北京联合大学科研项目(ZK70202005,ZK30202006);北京市高等学校高水平人才交叉培养“实培计划”项目;北京联合大学“启明星”大学生科技创新创业项目(201911417031,202011417SJ128)

Effects of astaxanthin combined with aerobic exercise on renal aging of rat induced by D-galactose and its mechanism

NIU Yan-long^1,2, CAO Jian-min³, ZHOU Qi-yun^4,5, HU Ge⁶, GUO Xian³, LIU Jian⁴, HAO Min⁴, ZHANG Tao³, ZHOU Hai-tao^4,5△

1. Gannan Medical University, Jiangxi Ganzhou 341000, China;
2. Key Laboratory of Rehabilitation Medicine in Ganzhou, Jiangxi Ganzhou 341000, China;
3. Beijing Sport University, Beijing 100084;
4. Beijing Union University, Beijing 100101;
5. Key Laboratory of Bioactive Substances and Functional Foods, Beijing Union University, Beijing 100191;
6. Changzhou University, Changzhou 213164, China

Online:2021-07-28 Published:2021-08-09

摘要/Abstract

摘要： 目的: 研究虾青素复合有氧运动对D-半乳糖诱导大鼠肾脏衰老的干预作用及其机制。方法: 60只3月龄SPF级SD大鼠采用两因素两水平2×2析因设计随机分为空白对照组(C组)、急性衰老组(S组)、虾青素+急性衰老组(AS组)、有氧运动+急性衰老组(ES组)、虾青素+有氧运动+急性衰老组(AES组),每组12只。大鼠腹腔注射100 mg/(kg·d) D-半乳糖复制经典急性衰老模型,并分别以20 mg/(kg·d) 虾青素和/或强度为60%最大摄氧量的有氧运动进行干预,实验周期6周。末次训练12 h后取肾脏,光镜/电镜观察肾脏组织形态/超微结构,酶联免疫吸附法检测肾脏组织超氧化物歧化酶(SOD)、γ-谷氨酸半胱氨酸合酶(γ-GCS)活性及丙二醛(MDA)含量,荧光比色法检测肾脏组织脂褐质(LDF)含量,免疫组化法检测肾脏组织核因子E2相关因子2(Nrf2)通路蛋白质表达水平。结果: 与AS、ES组比较:AES组肾脏组织形态/超微结构改善更为显著;LDF含量均显著降低(P＜0.01);SOD活性均显著升高(P＜0.01);γ-GCS活性显著高于AS组,而与ES组无显著性差异(P＞0.05);MDA含量组间无显著性差异(P＞0.05);Nrf2、磷酸化核因子E2相关因子2(p-Nrf2)蛋白质表达均显著升高(P＜0.05,P＜0.01);抗氧化酶血红素氧合酶1(HO-1)蛋白质表达显著高于ES组(P＜0.05),而与AS组无显著性差异(P＜0.05)。结论: 虾青素复合有氧运动可以延缓肾脏衰老,其作用机制可能为调控Nrf2信号通路相关蛋白质表达及下游Ⅱ相解毒酶和抗氧化酶活性,改善D-半乳糖致衰大鼠肾脏氧化应激。

关键词: 虾青素, 有氧运动, 衰老, 氧化应激, Nrf2, 肾

Abstract: Objective: To study the effects and mechanisms of astaxanthin combined with aerobic exercise on renal senescence of rat induced by D-galactose. Methods: Sixty 3-month-old SPF SD rats were divided into control group (C group), acute senescence group (S group), astaxanthin+acute senescence group (AS group), aerobic exercise+acute senescence group (ES group), astaxanthin+aerobic exercise+acute senescence group (AES group), by two-factor two-level 2×2 factorial design with 12 rats in each group. Acute senescence model of rat was establshed by intraperitoneal injection with 100 mg/(kg·d) D-galactose, and the intervention was conducted with 20 mg/(kg·d) astaxanthin and/or aerobic exercise with 60% VO_2max for 6 weeks. The histopathological/ultrastructural changes of the kidney were observed by light microscope/electron microscope; the levels of SOD, γ-GCS and MDA were detected by ELISA, and LDF in kidney was determined by fluorescence colorimetry; the protein expression of Nrf2 signaling pathway was detected by immunohistochemistry. Results: Compared with AS and ES group, in AES group, the improvement of renal tissue morphology/ultrastructure was more significant; LDF was decreased significantly (P＜0.01); SOD activity was significantly increased (P＜0.01); γ-GCS was significantly higher than that of AS group, but not significantly different from that of ES group (P＞0.05); there was no significant difference in MDA between groups (P＞0.05); the levels of Nrf2 and p-Nrf2 were increased significantly (P＜0.05, P＜0.01); HO-1 was significantly higher than that of ES group(P＜0.05), but not significantly different compared with that of AS group(P＞0.05). Conclusion: Astaxanthin combined with aerobic exercise can delay aging process of kidney, its mechanism may be that the combination regulate the protein expression in Nrf2 signaling pathway, Ⅱ detoxifying enzymes and antioxidant enzyme activity, and improve oxidative stress in kidney of rat induced by D-galactose.

Key words: astaxanthin, aerobic exercise, aging, oxidative stress, Nrf2, kidney

中图分类号:

G804.7

牛衍龙, 曹建民, 周绮云, 胡戈, 郭娴, 刘健, 郝敏, 张涛, 周海涛. 虾青素复合有氧运动对D-半乳糖诱导大鼠肾脏衰老的干预作用及其机制[J]. 中国应用生理学杂志, 2021, 37(4): 433-438.

NIU Yan-long, CAO Jian-min, ZHOU Qi-yun, HU Ge, GUO Xian, LIU Jian, HAO Min, ZHANG Tao, ZHOU Hai-tao. Effects of astaxanthin combined with aerobic exercise on renal aging of rat induced by D-galactose and its mechanism[J]. CJAP, 2021, 37(4): 433-438.

参考文献 21

[1]	叶朝阳, 崔琳琳. 老年人肾脏变化及功能评估[J]. 中华肾病研究电子杂志, 2015, 4(4): 182-186.
[2]	孟华. Nrf2: 老年心肾疾病的共同靶点[J]. 中国老年学杂志, 2013, 33(23): 6038-6040.
[3]	杨捷玲, 李琛, 刘青, 等. 连翘苷元对老年大鼠氧化应激和肾功能的改善作用[J]. 中国临床解剖学杂志, 2019, 37(1): 77-82.
[4]	Belviranli M , Okudan N. Effect of coenzyme Q10 alone and in combination with exercise training on oxidative stress biomarkers in rats[J]. Int J Vitam Nutr Res, 2019, 88(3-4): 1-11.
[5]	罗婧, 沈紫龙, 任建武. 虾青素对秀丽隐杆线虫衰老的改善作用及机制研究[J]. 食品工业科技, 2017, 38(22): 22-25.
[6]	Zhu XY, Chen YJ, Chen Q, et al. Astaxanthin promotes Nrf2/ARE signaling to alleviate renal fibronectin and collagen IV accumulation in diabetic Rats[J]. J Diabetes Res, 2018, (2018): 6730315.
[7]	Gounder SS, Kannan S, Devadoss D, et al. Impaired transcriptional activity of Nrf2 in age-related myocardial oxidative stress is reversible by moderate exercise training[J]. PLoS One, 2012, 7(9): e45697.
[8]	Yook JS, Rakwal R, Shibato J, et al. Leptin in hippocampus mediates benefits of mild exercise by an antioxidant on neurogenesis and memory[J]. Proc Natl Acad Sci USA, 2019, 116(22): 10988-10993.
[9]	李垚, 冯欣, 王雪鹰. 氯沙坦对D-半乳糖致衰老大鼠肾脏的保护作用[J]. 中国老年学杂志, 2010, 30(22): 3343-3345.
[10]	胡戈, 曹建民, 周海涛, 等. 虾青素介导Nrf2信号通路减轻大强度运动诱导大鼠心肌细胞的凋亡[J]. 食品工业科技, 2019, 40(8): 266-271.
[11]	Yeo W, Chan SL, Mo FK, et al. Phase I/II study of temsirolimus for patients with unresectable hepatocellular carcinoma (HCC)-a correlative study to explore potential biomarkers for response[J]. BMC Cancer, 2015, 15: 395-405.
[12]	Azman KF, Zakaria R. D-Galactose-induced accelerated aging model: an overview[J]. Biogerontology, 2019, 20(6): 763-782.
[13]	原慧萍, 杨泽. 氧化应激与衰老研究进展[J]. 中国老年保健医学, 2015, 13(5): 14-17.
[14]	吴媛妮, 尹晓晨, 李梓民, 等. D-半乳糖衰老模型致衰特征及衰老标志物的研究[J]. 中国热带医学, 2018, 18(7): 54-58.
[15]	Sandhu SK, Kaur G. Alterations in oxidative stress scavenge system in aging rat brain and lymphocytes[J]. Biogerontology, 2002, 3(3): 161-173.
[16]	Nguyen T, Nioi P, Pickett CB. The Nrf2-antioxidant responseelement signaling pathway and its activation by oxidative stress[J]. J Biol Chem, 2009, 284(20): 13291-13295.
[17]	Sorrenti V, Davinelli S, Scapagnini G, et al. Astaxanthin as a putative geroprotector: Molecular basis and focus on brain aging [J]. Mar Drugs, 2020, 18(7): 351.
[18]	Sztretye M, Dienes B, Gnczi M, et al. Astaxanthin: A potential mitochondrial-targeted antioxidant treatment in diseases and with aging[J]. Oxid Med Cell Longev, 2019, 11: 3849692.
[19]	Navarro A, Gomez C, Lopez-Cepero JM, et al. Beneficial effects of moderate exercise on mice aging: survival, behavior, oxidative stress, and mitochondrial electron transfer[J]. Am J Physiol Regul Integr Comp Physiol, 2004, 286(3): R505-R511.
[20]	李洁, 谈文博, 王艳. 有氧运动对衰老大鼠骨骼肌线粒体能量代谢的影响[J]. 中国应用生理学杂志, 2018, 34(3): 234-237.
[21]	李丹, 王世强. 运动对Keap1/Nrf2信号通路增强糖尿病大鼠心肌抗氧化能力的研究[J].中国糖尿病杂志, 2019, 27(5): 386-392.

虾青素复合有氧运动对D-半乳糖诱导大鼠肾脏衰老的干预作用及其机制

Effects of astaxanthin combined with aerobic exercise on renal aging of rat induced by D-galactose and its mechanism

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