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中国应用生理学杂志 ›› 2021, Vol. 37 ›› Issue (6): 665-672.doi: 10.12047/j.cjap.6065.2021.058

• 研究论文 • 上一篇    下一篇

有氧运动联合螺旋藻多糖对糖尿病大鼠学习记忆能力的影响及其机制*

朱洪竹, 朱梅菊, 张莹   

  1. 井冈山大学体育学院, 江西 吉安 343009
  • 收稿日期:2020-03-30 修回日期:2021-02-01 出版日期:2021-11-28 发布日期:2021-11-25
  • 通讯作者: Tel: 0796-8100491; E-mail: zhuhongzhu2007@163.com
  • 基金资助:
    *国家自然科学基金资助项目(31660291,31360255);江西省自然科学基金资助项目(20192BAB205092,20202BABL206124);江西省教育厅科技计划资助项目(GJJ190562)

The effects of aerobic exercise plus spirulina polysaccharide supplement on learning and memory function of diabetes rats and its mechanism

ZHU Hong-zhu, ZHU Mei-ju, ZHANG Ying   

  1. School of PE and Sports Science, Jinggangshan University, Ji'an 343009, China
  • Received:2020-03-30 Revised:2021-02-01 Online:2021-11-28 Published:2021-11-25

摘要: 目的:研究有氧运动联合螺旋藻多糖对糖尿病大鼠学习记忆能力及对海马脑组织p75NTR信号相关蛋白的影响。方法:采用高糖高脂饮食喂养4周配合低剂量腹腔注射的方法复制Ⅱ型糖尿病大鼠实验模型。成模后随机分为:模型组(B组)、运动+糖尿病组(C组)、螺旋藻多糖+糖尿病组(D组)、运动+螺旋藻多糖+糖尿病组(E组),另设正常对照组(A组)。共5组,每组12只。C组和E组施加6周的有氧游泳训练,D组和E组给予螺旋藻多糖灌胃6周,A组不施加任何干预。用Morris水迷宫检测大鼠学习记忆能力;Tunel染色法检测神经元细胞凋亡情况;ELISA法检测BDNF含量,Western blot法检测p75NTR和cleaved caspase-3蛋白表达,免疫组化法检测cleaved caspase-3表达变化。同时观察大鼠随机血糖、血胰岛素等指标的变化。结果:①与A组比较,B组不同时间点上的体重均显著降低(P<0.01);与B组比较,C、D、E组在不同时间点上的体重差异均无统计学意义(P均>0.05)。与A组比较,B组的血糖和胰岛素水平显著升高(P<0.01);与B组比较,干预各组的血糖和胰岛素水平显著降低(P<0.05或P<0.01)。②与A组比较,B组寻找平台的逃避潜伏期明显延长(P<0.01),目标象限时间和穿越平台次数显著减少(P<0.01);与B组比较,各干预组逃避潜伏期均显著缩短(P<0.05或P<0.01),穿越平台次数均显著增加(P<0.05或P<0.01),其中以E组效果最好。③与B组比较,干预各组海马神经细胞凋亡减少,p75NTR、cleaved caspase-3蛋白表达显著降低(P<0.05或P<0.01),BDNF含量明显增加(P<0.05或P<0.01)。其中以E组效果更为明显。结论:有氧运动与螺旋藻多糖能有效改善糖尿病大鼠学习记忆,其中以两者联合组的效果更为显著,其机制可能与其更好的调节p75NTR信号相关蛋白的表达,一定程度抑制细胞凋亡,从而有效改善Ⅱ型糖尿病学习记忆,发挥神经保护作用有关。

关键词: 有氧运动, 螺旋藻多糖, p75NTR信号, 细胞凋亡, 学习记忆

Abstract: Objective: To investigate the effects of aerobic exercise plus spirulina polysaccharide(SP) supplement on the related protein expressions of p75NTR signal in hippocampal and the improvement of learning and memory of type 2 diabetes rats. Methods: The model of type 2 diabetic rats was established by fed high-fat diet for four weeks together with intraperitoneal injecting a low dose of STZ. The model rats were randomly divided into diabetic model group(B),diabetic exercise group(C),diabetic+SP group(D)and diabetic exercise+SP group(E), another normal control group(A)without any intervention was set up,12 rats in each group. The rats in Group C and E were treated with intervention of swimming training for six weeks. The rats in Group D and E were treated with SP intragastrically for 6 weeks. Learning and memory abilities were observed by Morris water maze test. The hippocampus cell apoptosis was observed by Tunnel staining, and BDNF content and the expressions of p75NTR, cleaved caspase-3 of hippocampus were tested by ELISA, Western blot and immunohistochemistry, respectively. At the same time, the changes of blood glucose and levels of serum insulin were examined. Results: ①Compared with Group A at different time points, the body weight of Group B was decreased significantly(P<0.01). Compared with Group B at different time points, the body weight of Group C,D and E had no difference (P>0.05). Compared with Group A, levels of the blood glucose and serum insulin Group B were increased significantly(P<0.01).Compared with Group B, the levels in the intervention groups were decreased significantly (P<0.01); ②Compared with Group A, the escape latencies of Group B were prolonged significantly(P<0.01), platform quadrant residence duration and the times of crossing platform were decreased (P<0.01). Compared with Group B, the escape latencies of the intervention groups were shortened (P<0.05 or P<0.01), and the times of crossing platform were increased (P<0.05 or P<0.01). ③Compared with Group B, the neural cells apoptosis of the intervention rats was decreased, and the protein expressions of p75NTR and cleaved caspase-3 were decreased (P<0.05 or P<0.01), however the expression of BDNF was increased significantly (P<0.05 or P<0.01). Conclusion: Aerobic exercise and SP supplement can improve the learning-memory ability of type 2 diabetes rats, and the improvement effect of exercise combined with SP is markedly better than that of exercise and SP alone, the mechanism might be related to better regulating p75NTR signal related protein expressions and then inhibiting apoptosis in hippocampus of rats with type 2 diabetes.

Key words: aerobic exercise, spirulina polysaccharide, p75NTR signal, cell apoptosis, learning and memory

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