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中国应用生理学杂志 ›› 2021, Vol. 37 ›› Issue (4): 419-422.doi: 10.12047/j.cjap.6078.2021.039

• 研究论文 • 上一篇    下一篇

不同浓度桦木酸对人胃癌MGC-803细胞凋亡的影响

樊华1, 邵淑丽1,2Δ, 何孟奇1, 黄鑫1,2, 张伟伟1,2, 张珍珠1,2   

  1. 1. 齐齐哈尔大学生命科学与农林学院, 黑龙江 齐齐哈尔 161006;
    2. 抗性基因工程与寒地生物多样性保护黑龙江省重点实验室, 黑龙江 齐齐哈尔 161006
  • 出版日期:2021-07-28 发布日期:2021-08-09
  • 通讯作者: Tel: 0452-2738219; E-mail: shshl32@163.com
  • 基金资助:
    *齐齐哈尔大学黑龙江省教育厅基本业务专项重点项目(135109104);黑龙江省高教强省优势特色学科—玉米“粮头食尾”重点专项(LTSW201737);黑龙江省省属高等学校基本科研业务费科研项目(植物性食品加工技术特色学科专项,YSTSXK201809)

Effects of betulinic acid on apoptosis of human gastric cancer MGC-803 cells

FAN Hua1, SHAO Shu-li 1,2Δ, HE Meng-qi1, HUANG Xin 1,2, ZHANG Wei-wei1,2, ZHANG Zhen-zhu1,2   

  1. 1. College of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihaer 161000;
    2. Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar University, Qiqihaer 161000, China
  • Online:2021-07-28 Published:2021-08-09

摘要: 目的: 以人胃癌MCG-803细胞为实验材料,探讨不同浓度桦木酸(BA)对人胃癌MGC-803细胞凋亡的影响,为其临床应用提供依据。方法: 将人胃癌MGC-803细胞分成 4 组,每组设置 3 个复孔,对照组为未加入桦木酸的 MGC-803 细胞,3 组实验组分别加入终浓度为10、20、30 μg/ml桦木酸处理细胞48 h后,通过激光共聚焦显微镜观察各组细胞形态变化;检测桦木酸对细胞Caspase-3和Caspase-9活性的影响;利用流式细胞术检测细胞线粒体膜电位变化;qRT-PCR和Western blot检测凋亡相关基因Caspase-3、Caspase-9Cytochrome C(Cyt c)mRNA及蛋白水平的表达变化。结果: 与对照组比较, 各处理组Caspase-3和Caspase-9活性显著升高(P<0.01),线粒体膜电位显著下降(P<0.01),Caspase-3、Caspase-9和Cyt c mRNA及蛋白表达均显著上调(P<0.01)。结论: 在终浓度为10 ~30 μg/ml浓度范围内,桦木酸通过调节Caspase-3、Caspase-9和Cyt c的表达诱导人胃癌MGC-803细胞凋亡。

关键词: 桦木酸, 人胃癌MGC-803细胞, 细胞凋亡

Abstract: Objective: The effects of betulinic acid (BA) on apoptosis of human gastric cancer MGC-803 cells was investigated by using human gastric cancer MGC-803 cells as experimental materials, and the basis for its clinical application was provided. Methods: The human gastric cancer MGC-803 cells were divided into 4 groups,each group was set with 3 replicates.The control group was MGC-803 cells without being added betulinic acid; the other 3 groups of experimental groups were treated with betulinic acid at final concentrations of 10, 20 and 30 μg /ml respectively. Cells were treated with betulinic acid of different concentrations for 48 h. Laser confocal microscope was used to observe morphological changes of MGC-803. The activities of Caspase-3 and Caspase-9 were detected by an assay kit. Flow cytometry was applied to determine mitochondrial membrane potential. The mRNA and protein levels of Caspase-3, Caspase-9 and Cyt c were also detected by qRT-PCR and Western blot, respectively. Results: Compared with the control group, the activities of Caspase-3 and caspase-9 were increased(P<0.01), while the mitochondrial membrane potential was decreased significantly(P<0.01). The mRNA and protein expressions of Caspase-3, caspase-9 and Cyt c were up-regulated significantly(P<0.01). Conclusion: In the final concentration range of 10 ~ 30 μg/ml, betulinic acid can induce apoptosis of human gastric cancer MGC-803 cells by regulating the expression of Caspase-3, Caspase-9 and Cyt c.

Key words: betulinic acid, human gastric cancer MGC-803, cell apoptosis

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