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中国应用生理学杂志 ›› 2021, Vol. 37 ›› Issue (5): 495-499.doi: 10.12047/j.cjap.6091.2021.060

• 研究论文 • 上一篇    下一篇

桦木酸对胃癌SGC-7901细胞凋亡的影响*

丁璐1, 邵淑丽1,2△, 何孟奇1, 黄鑫1,2, 张伟伟1,2, 张珍珠1,2   

  1. 1.齐齐哈尔大学生命科学与农林学院;
    2.抗性基因工程与寒地生物多样性保护黑龙江省重点实验室, 黑龙江 齐齐哈尔 161006
  • 收稿日期:2020-05-14 修回日期:2021-02-02 出版日期:2021-09-28 发布日期:2021-11-24
  • 通讯作者: Tel: 0452-2738219; E-mail: shshl32@163.com
  • 基金资助:
    *齐齐哈尔大学黑龙江省教育厅基本业务专项重点项目(135109104);黑龙江省高教强省优势特色学科—玉米“粮头食尾”重点专项(LTSW201737);黑龙江省省属高等学校基本科研业务费科研项目(植物性食品加工技术特色学科专项 YSTSXK201809)

Effects of Betulinic acid on apoptosis in human gastric cancer SGC-7901 cells

DING Lu1, SHAO Shu-li1,2, HE Meng-qi1, HUANG Xin 1,2, ZHANG Wei-wei1,2, ZHANG Zhen-zhu1,2   

  1. 1. College of Life Science and Agriculture and Forestry;
    2. Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar University, Qiqihaer 161000, China
  • Received:2020-05-14 Revised:2021-02-02 Online:2021-09-28 Published:2021-11-24

摘要: 目的: 以人胃癌SGC-7901细胞为研究对象,探究桦木酸对其凋亡的影响。方法: 将人胃癌SGC-7901细胞分为4组,每组设置3个复孔,对照组未加入桦木酸,而三组实验组分别加入浓度为10 mg/L、20 mg/L及30 mg/L的桦木酸,将各组细胞放入5%的CO2培养箱中培养48 h,激光共聚焦显微镜观察细胞形态变化;流式细胞术检测细胞凋亡率和线粒体膜电位变化;qRT-PCR和Western blot分别检测SGC-7901细胞凋亡相关基因Bcl-2BaxCaspase-3在mRNA和蛋白水平的表达。结果: 与对照组相比,终浓度为10 mg/L、20 mg/L、30 mg/L的桦木酸处理组,细胞发生皱缩、细胞核裂解并出现凋亡小体;细胞早期凋亡与晚期凋亡率显著增加(P<0.05 or P<0.01),线粒体膜电位明显降低(P<0.05 or P<0.01);细胞凋亡相关基因BaxCaspase-3的mRNA与蛋白表达水平均显著上升(P<0.01),而Bcl-2的mRNA与蛋白表达水平显著降低(P<0.01)。结论: 在一定浓度范围内,桦木酸通过调节凋亡相关基因Bcl-2BaxCaspase-3的表达诱导人胃癌SGC-7901细胞凋亡。

关键词: 桦木酸, 人胃癌SGC-7901细胞, 细胞凋亡

Abstract: Objective: To investigate the effects of betulinic acid on apoptosis of human gastric cancer SGC-7901 cells. Methods: The human gastric cancer SGC-7901cells were divided in to 4 groups, and each group was set with 3 replicates. The SGC-7901cells in control group were not treated with betulinic acid; the other 3 experimental groups were treated with betulinic acid at the concentrations of 10, 20 and 30 mg/L, respectively; each group was incubated in a 5% carbon dioxide incubator for 48 h. Laser confocal microscope was used to observe morphological changes of SGC-7901 cells; Flow cytometry was applied to determine apoptosis rate and mitochondrial membrane potential. The mRNA and protein levels of Bcl-2, Bax and Caspase-3 were also detected by qRT-PCR and western blot respectively. Results: Compared with the control group, SGC-7901 cells in the treated group at final concentrations of 10, 20 and 30 mg/L shrinked, appeared apoptosis body along with nuclear splitting. The percentage of cells in early and advanced period of apoptosis were markedly increased (P<0.05 or P<0.01), mitochondrial membrane potential was obviously reduced (P<0.05 or P<0.01). qRT-PCR and western blot analysis showed that the mRNA and protein expressions of Bax and Caspase-3 were increased significantly (P<0.01), while the expressions of Bcl-2 were decreased significantly (P<0.01). Conclusion: Within a certain range of concentrations, betulinic acid induces cell apoptosis by regulating the expression of Bcl-2, Bax and Caspase-3 in human gastric cancer.

Key words: betulinic acid, human gastric carcinoma SGC-7901 cells, cell apoptosis

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