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中国应用生理学杂志 ›› 2021, Vol. 37 ›› Issue (4): 439-444.doi: 10.12047/j.cjap.6094.2021.043

• 研究论文 • 上一篇    下一篇

间歇运动对心梗大鼠心肌氧化应激和炎症的影响及其机制

耿元文1, 林琴琴1△, 王湘怡1, 李若明1, 田振军2   

  1. 1. 燕山大学 体育学院, 河北 秦皇岛 066004;
    2. 陕西师范大学 体育学院, 西安 710062
  • 出版日期:2021-07-28 发布日期:2021-08-09
  • 通讯作者: Tel: 0335-8057020; E-mail: linqinqin@ysu.edu.cn
  • 基金资助:
    *国家自然科学基金项目(31300978);河北省自然科学基金(C2019203537);河北省高等学校科学技术研究项目(QN2019068)

Effects of aerobic interval training on myocardial oxidative stress and inflammation in rats with myocardial infarction and its mechanism

GENG Yuan-wen1, LIN Qin-qin1△, WANG Xiang-yi1, LI Ruo-ming1, TIAN Zhen-jun2   

  1. 1. Yanshan University, Qinhuangdao 066004;
    2. Shaanxi Normal University, Xi'an 710062, China
  • Online:2021-07-28 Published:2021-08-09

摘要: 目的: 探讨间歇运动激活心肌梗死(MI)大鼠SIRT1-Nox4-ROS通路抑制心脏氧化应激和炎症的作用。方法: 选取雄性SD 大鼠30 只,随机分为假手术对照(C)组,心肌梗死(MI) 组和心梗+间歇运动(ME)组,每组10 只。MI 组采用心脏左冠状动脉前降支(LAD) 结扎法,建立MI 模型。C组大鼠实施假手术,ME 组大鼠在MI 手术后1 周进行4 周跑台运动。第一周为适应性训练(10~15 m/min,30 min/d,共5 d)。正式训练起始训练速度10 m/min,时间为10 min。速度逐渐增至25 m/min,运动7 min后,间歇3 min,其速度为15 m/min,之后依次交替进行,运动总时间为60 min,5 d/周 ×4 周。训练结束后测定各组大鼠心电图变化,开胸摘取心脏。HE染色检测心肌组织结构,紫外分光光度法测定心脏MDA含量和SOD活性,微量酶标法测定心脏LDH活性,RT-qPCR 检测心肌SIRT1 mRNA表达,Western blot 检测心肌SIRT1、Nox4、TNF-α和IL-1β蛋白表达,DHE 法检测心肌活性氧(ROS)水平。结果: 与C组比较,MI组大鼠心肌组织出现代替性纤维化,心脏Nox4蛋白表达显著增加 (P<0.01),MDA含量、LDH活性和ROS水平显著升高(P<0.01),SOD活性显著降低 (P<0.01),TNF-α和IL-1β蛋白表达显著增加 (P<0.01),SIRT1 mRNA和蛋白表达显著降低 (P<0.01)。与MI组比较,ME组大鼠心肌纤维化降低,心脏Nox4蛋白、MDA含量、LDH活性和ROS水平显著降低 (P<0.01),SOD活性显著增强 (P<0.01),TNF-α和IL-1β蛋白表达显著降低 (P<0.01),SIRT1 mRNA和蛋白表达明显增多 (P<0.01)。且发现SIRT1与Nox4及ROS 水平均呈显著负相关(r分别为-0.925和-0.899,P均<0.01)。结论: 间歇运动抑制心梗心脏氧化应激和炎症反应,改善心功能,其机制与SIRT1-Nox4-ROS信号通路激活密切相关。

关键词: 心肌梗死, 间歇运动, SIRT1, 氧化应激, 炎症, 大鼠

Abstract: Objective: The present study was to determine the effects of aerobic interval training (AIT) on the expressions of SIRT1, Nox4 and inflammatory factor in the heart of rats with myocardial infarction (MI). Methods: Male Sprague Dawley rats were randomly divided into sham-operated group (C), sedentary MI group (MI) and MI with AIT group (ME) (n=10). The MI model was established by ligation the left anterior descending coronary artery. Rats in C groups were subjected to the same surgery, but only threaded and not ligated. After surgery 1 week, rats in ME groups took adaptability training for 1 week, and then subjected to 4 weeks treadmill exercise training. After training, the hearts were collected for histological observation. The level of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) in heart was assessed by ultraviolet spectrophotometry. The activity of lactate dehydrogenase (LDH) was determined by enzyme linked immunosorbent assay. The expression of sirtuin1 (SIRT1) mRNA was examined by real-time quantitative polymerase chain reaction (RT-qPCR). The protein expressions of SIRT1, NADPH oxidase 4 (Nox4), tumor necrosis factor-alpha (TNF-α) and interleukin-1β (IL-1β) were detected by Western blotting. The levels of reactive oxygen species (ROS) were detected by dihydroethidium (DHE) staining. Results: Compared with the C group, the expression level of cardiac Nox4 protein was increased (P<0.01), the level of MDA, activity of LDH and the level of ROS were increased significantly (P<0.01), and the expressions of TNF-α and IL-1β protein were augmented in the heart of rats with MI (P<0.01). However, the expressions of SIRT1 mRNA and protein and the activity of SOD were obviously decreased in MI group (P<0.01). Furthermore, compared with the MI group, AIT increased the expressions of SIRT1 mRNA and protein and the activity of SOD in the heart of ME group (P<0.01); Meanwhile, the expressions of cardiac Nox4, MDA level, LDH activity and ROS level were diminished in ME group (P<0.01) as well as the decreased expressions of TNF-α and IL-1β protein (P<0.01). SIRT1 expression was negatively related to the expressions of NOX4 and ROS. Conclusion: AIT obviously inhibited myocardial oxidative stress and inflammatory reaction, improved cardiac function in rats with MI, and the mechanism was closely related to the activation of SIRT1-Nox4-ROS signaling pathway.

Key words: myocardial infarction, aerobic interval training, SIRT1, oxidative stress, inflammation, rats

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