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中国应用生理学杂志 ›› 2021, Vol. 37 ›› Issue (6): 694-698.doi: 10.12047/j.cjap.6099.2021.090

• 研究论文 • 上一篇    下一篇

归脾汤对大鼠心肌缺血的干预作用*

吴江立1, 王晓影1, 代成1, 李睿嘉1, 张越1,2, 孙佳欢1,2, 李爱英1,2△   

  1. 1.河北中医学院, 石家庄 050200;
    2.河北省心脑血管病中医药防治研究重点实验室, 石家庄 050091
  • 收稿日期:2020-05-28 修回日期:2021-02-18 出版日期:2021-11-28 发布日期:2021-11-25
  • 通讯作者: Tel: 18931366351; E-mail: aiyingli1963@163.com
  • 基金资助:
    *国家自然科学基金(81573698);河北中医学院大学生创新创业训练计划项目(201814432004)

Effects of Gui pi tang on myocardial ischemia in rats

WU Jiang-li1, WANG Xiao-ying1, DAI Cheng1, LI Rui-jia1, ZHANG Yue1,2, SUN Jia-huan1,2, LI Ai-ying1,2△   

  1. 1. Hebei University of Chinese Medicine, Shijiazhuang 050020;
    2. Hebei Key Laboratory of Chinese Medicine Research on Cardio-cerebrovascular Disease, Shijiazhuang 050091, China
  • Received:2020-05-28 Revised:2021-02-18 Online:2021-11-28 Published:2021-11-25

摘要: 目的:观察归脾汤(GPT)对心肌缺血大鼠的保护作用。方法:将40只SD大鼠随机分为5组(n=8):空白对照组(Control),模型组(Model)、归脾汤低剂量组(Gpt 7.52 g/kg)、归脾汤高剂量组(Gpt 15.04 g/kg),阳性对照药曲美他嗪组(Trimetazidine,2 mg/kg)。应用饲喂高脂饮食联合注射异丙肾上腺素(ISO)的方法建立大鼠心肌缺血模型,灌胃给药15 d后,各组再次腹腔注射ISO 3 d,随后大鼠进行心电图检测,取材,检测血液中甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、肌酸激酶(CK)、乳酸脱氢酶(LDH)和葡萄糖(GLU)的变化,HE及Masson染色观察心肌病理改变,Western blot检测心肌组织中CollagenⅠ和CollagenⅢ的蛋白表达。结果:与正常对照组相比,模型组大鼠心电图出现S-T段下移,血清TC、AST、CK、LDH和GLU水平显著升高(P<0.05),心肌组织CollagenI和CollagenIII蛋白表达显著升高(P<0.05),心肌受损严重。TG,HDL-C,LDL-C和ALT变化不显著(P>0.05)。与模型组相比,归脾汤低、高剂量组和曲美他嗪能抑制心肌缺血大鼠心电图S-T段下移,降低血清中TC、AST、CK、LDH和GLU水平(P<0.05),降低心肌组织CollagenⅢ的表达(P<0.05),减轻心肌病理损伤程度,归脾汤高剂量组可显著降低CollagenⅠ的蛋白表达(P<0.05)。结论:归脾汤具有改善心脏功能,减轻心肌缺血损伤的作用,尤以高剂量效果更为显著。

关键词: 心肌缺血, 大鼠, 归脾汤, 胶原蛋白

Abstract: Objective: To investigate the effects of Guipitang (GPT) on myocardial ischemic (MI) injury of rats. Methods: Forty male SD rats were randomly divided into five groups as control, model, GPT low-dose and high-dose groups (7.52, 15.04 g/kg), and positive-drug trimetazidine group (2 mg/kg). Rat myocardial ischemia model was induced by feeding high fat forage and intraperitoneal injection of isoprenaline (ISO). After 15 days intragastric administration, rats were injected with ISO once a day for 3 days again. Subsequently, Electrocardiograph (ECG) was examined, serum total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), creatine kinase (CK), lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and glucose (GLU) were detected using an automatic biochemical analyzer. The histopathological alterations of heart were assessed using HE and Masson staining. The protein expressions of Collagen I and Collagen III in heart were evaluated by Western blot. Results: Compared with control group, the electrocardiogram S-T segment of model rats moved down, the serum levels of TC, AST, CK, LDH and GLU in model group were increased significantly (P<0.05), the expressions of collagen I and collagen III in heart were increased (P<0.05), and the hearts were damaged severely. However, no significant changes of TG, HDL-C, LDL-C and ALT were observed (P>0.05). Compared with the model group, the high and low dose groups of GPT and trimetazidine could inhibit the descent of S-T segment, reduced serum TC, AST, CK, LDH and GLU levels (P<0.05), and decreased collagen III expression in heart (P<0.05), and alleviated myocardial pathological damage as well. The high dose group of GPT could decrease the protein expression of collagen I. Conclusion: GPT could improve heart function and alleviate the injury of myocardial ischemia, especially the high lose.

Key words: myocardial ischemic, rat, Gui pi tang, collagen

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