蒙药绍沙-7味丸防治大鼠心肌缺血/再灌注损伤的作用

doi:10.12047/j.cjap.6114.2021.029

中国应用生理学杂志 ›› 2021, Vol. 37 ›› Issue (4): 380-384.doi: 10.12047/j.cjap.6114.2021.029

蒙药绍沙-7味丸防治大鼠心肌缺血/再灌注损伤的作用

杨向君^1,2+, 王羽³⁺, E.巴雅日玛⁴, 赵明^2△, S.贺希格扎日嘎拉^1△

1. 蒙古国国立医科大学国际蒙医学院, 乌兰巴托 999097-15160, 蒙古;
2. 内蒙古民族大学附属医院, 通辽 028002, 中国;
3. 内蒙古民族大学医学院, 通辽 028000, 中国;
4. 蒙古国国立医科大学生物医学院, 乌兰巴托 999097-15160, 蒙古

出版日期:2021-07-28 发布日期:2021-08-09
通讯作者: ^△Tel: 13474859991; E-mail: langzhe73@163.com, khishigjargals@yahoo.com.⁺: 共同第一作者
基金资助:
^*国家自然科学基金项目(81360587);内蒙古民族大学附属医院青年科研启动项目(2019QNJJ05);内蒙古自治区高等学校“青年科技英才支持计划”(NJYT-19-B14)

Effects of Mongolian medicine Shaosha-7 on myocardial ischemia/ reperfusion injury of rats

YANG Xiang-jun^{1, 2+}, WANG Yu³⁺, E.bayarima⁴, ZHAO Ming^2△, S. hexigezarigala^1△

1. MNUMS, International School of Mongolian Medicine, Mongolian National University of Medical Sciences, Ulan Bator 999097-15160, Mongolian;
2. Affiliated Hospital of Inner mongolia University for National, Tongliao 028002, China;
3. Medical college of Inner mongolia University for Nationalities, Tongliao 028000, China;
4.MNUMS, School of Biomedicine,Mongolian National University of Medical Sciences, Ulan Bator 999097-15160, Mongolian

Online:2021-07-28 Published:2021-08-09

摘要/Abstract

摘要： 目的: 探讨蒙药绍沙-7味丸对心肌缺血/再灌注损伤大鼠的防治作用及机制。方法: 60只大鼠随机分成6组:假手术组、模型组、蒙药绍沙-7味丸低、中、高剂量组以及阳性药对照组,每组10只;蒙药绍沙-7味丸低、中、高剂量组分别灌胃0.4 g/kg、0.8 g/kg、1.6 g/kg蒙药绍沙-7味丸,阳性药对照组灌胃0.3 g/kg复方丹参片,假手术组和模型组灌胃2 ml/100 g蒸馏水,均连续灌胃15 d,1次/日;15 d后,模型组及治疗组大鼠开胸结扎冠状动脉30 min后,解开结扎线行再灌注120 min后关闭胸腔处理;6组大鼠术后行心电图检查后处死大鼠取心脏行HE染色、TTC染色,观察梗死面积及病理变化。ELISA法检测血清中cTnI、CK-MB、 LDH、MDA、SOD、GSH-PX及TNF-α、IL-18、IL-6、IL-1β的水平,免疫组化法检测心肌组织NF-кB水平。结果: 与假手术组相比,心肌缺血/再灌注损伤大鼠心肌梗死面积增大,同时血清中cTnI、CK-MB、 LDH、MDA、GSH-PX及TNF-α、IL-18、IL-6、IL-1β的含量明显增加(P＜0.01),SOD的含量则明显降低(P＜0.01),心脏组织中的NF-кB水平增加;与心肌缺血/再灌注损伤大鼠相较,蒙药绍沙-7味丸改善大鼠心律失常现象及病理变化,减少梗死面积;血清中cTnI、CK-MB、 LDH、MDA、GSH-PX及TNF-α、IL-18、IL-6、IL-1β的含量明显降低(P＜0.01),SOD的含量明显增加(P＜0.01),心脏组织中的NF-кB水平降低。结论: 蒙药绍沙-7味丸可有效缓解大鼠心肌缺血/再灌注损伤,其作用与氧化应激和凋亡相关。

关键词: 蒙药绍沙-7味丸, 心肌缺血再灌注损伤, 氧化应激, 炎症, 大鼠

Abstract: Objective: To investigate the effects of Shaosha-7 in rats with myocardial ischemia-reperfusion injury and its mechanisms. Methods: male SD rats were divided into sham operation group (n=10), myocardial ischemia-reperfusion injury group (n=10), low, medium and high dose of Shaosha-7 groups (n=10), and positive drug group (n=10). The rats of Shaosha-7 (low, medium and high dose) groups were treated with Shaosha-7 at the doses of 0.4, 0.8 and 1.6 g/kg respectively, once a day for 15 days. The rats of positive drug group were treated with 0.3 g/kg Danshen, once a day for 15 days. The rats of the sham operation group and myocardial ischemia-reperfusion injury were treated with 2 ml/100 g distilled water, once a day for 15 days. After 15 days, the rats of the model group and the treatment group underwent thoracotomy and ligation of coronary artery for 30 minutes, then thoracic cavity was closed after reperfusion. Rats in six groups were executed electrocardiographic examination and their hearts were taken for Hematoxylin-Eosin (HE) staining and 2, 3, 5-triphenyltetrazolium chloride (TTC) staining to observe infarct area and myocardial pathological changes. The contents of cTnI, CK-MB, LDH, MDA, SOD, GSH-Px, TNF-α, IL-18, IL-6 and IL-1 β in serum were detected by ELISA. The expression of NF-кB was detected by immunohistochemistry. Results: Compared with the sham operation group, the infarct size, the levels of cTnI, CK-MB, CK-MB, LDH, MDA, GSH-Px, TNF-α, IL-18, IL-6, IL-1β and NF-кB were increased and the content of SOD were decreased in rats with myocardial ischemia-reperfusion injury. Compared with the rats with myocardial ischemia-reperfusion injury, Shaosha-7 improved the arrhythmia and pathological changes, reduced the infarct area, decreased the contents of cTnI, CK-MB, LDH, MDA, GSH-Px, TNF-α, IL-18, IL-6, IL-1 β, increased the content of SOD, decreased the expression of NF-кB. Conclusion: Mongolian medicine Shaosha-7 can effectively alleviate myocardial ischemia-reperfusion injury in rats. This study provides a theoretical basis for the treatment of myocardial ischemia-reperfusion (I/R) injury with Shaosha-7.

Key words: Mongolian medicine shaosha-7, myocardial ischemia-reperfusion injury, oxidative stress, inflammation, rats

中图分类号:

R331.3
R332

杨向君, 王羽, E.巴雅日玛, 赵明, S.贺希格扎日嘎拉. 蒙药绍沙-7味丸防治大鼠心肌缺血/再灌注损伤的作用[J]. 中国应用生理学杂志, 2021, 37(4): 380-384.

YANG Xiang-jun, WANG Yu, E.bayarima, ZHAO Ming, S. hexigezarigala. Effects of Mongolian medicine Shaosha-7 on myocardial ischemia/ reperfusion injury of rats[J]. CJAP, 2021, 37(4): 380-384.

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蒙药绍沙-7味丸防治大鼠心肌缺血/再灌注损伤的作用

Effects of Mongolian medicine Shaosha-7 on myocardial ischemia/ reperfusion injury of rats

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