不同频率的振动训练对大鼠早期膝骨关节炎关节软骨的修复作用及其JNK/NF-κB、SOX9机制*

doi:10.12047/j.cjap.6204.2022.008

中国应用生理学杂志 ›› 2022, Vol. 38 ›› Issue (1): 41-46.doi: 10.12047/j.cjap.6204.2022.008

不同频率的振动训练对大鼠早期膝骨关节炎关节软骨的修复作用及其JNK/NF-κB、SOX9机制^*

汪宗保^1,2,3△, 王连³, 柳奇奇¹, 杨永晖², 刘攀², 李斯亮¹, 姚长风¹

1.安徽中医药大学康复医学院, 合肥 230038;
2.安徽中医药大学第三附属医院, 合肥 230031;
3.上海体育学院运动科学学院, 上海 200438

收稿日期:2021-04-08 修回日期:2022-01-06 出版日期:2022-01-28 发布日期:2022-05-30
通讯作者: ^△Tel: 0551-62736630; E-mail: 673099692@qq.com
基金资助:
* 安徽省自然科学基金项目(1508085MH181);安徽省卫生健康委科研项目(AHWJ2021b052)

Repair impact of vibration exercise with different frequencies on articular cartilage of rats with early knee osteoarthritis and its JNK/NF-κB, SOX9 mechanisms

WANG Zong-bao^1,2,3△, WANG Lian³, LIU Qi-qi¹, YANG Yong-hui², LIU Pan², LI Si-liang¹, YAO Chang-feng¹

1. School ofRehabilitation Medicine,Anhui University of Chinese Medicine,Hefei 230038;
2. The Third Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230031;
3. School of Sports Science, Shanghai University of Sport, Shanghai 200438, China

Received:2021-04-08 Revised:2022-01-06 Online:2022-01-28 Published:2022-05-30

摘要/Abstract

摘要： 目的: 探讨不同频率的振动训练对大鼠早期膝骨关节炎关节软骨的修复作用及其JNK/NF-κB、SOX9的机制。方法: 成年雄性SD大鼠48只,随机分为6组(n=8):模型对照组(MC组),高频振动1组(GP₁组,频率60 Hz),高频振动2组(GP₂组,频率40 Hz)、中频振动组(ZP组,频率20 Hz)和低频振动组(DP组,频率10 Hz),正常对照组(NC组)。除正常组外,各组大鼠经第1、4、7日双后腿膝关节腔注射2%木瓜蛋白酶溶液和L-半胱氨酸混合液6周后建立早期膝骨关节炎模型后,对振动组大鼠双膝进行4周,每天 40 min,振幅2~5 mm,每周振动5 d的训练。4周后,检测双膝关节股骨外侧髁关节软骨HE染色、番红O染色和Mankin评分形态学观察,股骨内侧髁关节软骨RT-qPCR检测JNK、NF-κB p65、SOX9 mRNA,Western blot 检测JNK、NF-κB p65、SOX9蛋白表达。结果: 与NC比较,其余各组Mankin评分均显著增高(P＜0.01),与MC相比,各振动组Mankin评分均显著降低(P＜0.05),其JNK、NF-κB p65mRNA和蛋白质表达显著降低(P＜0.01),SOX9mRNA和蛋白质表达显著升高(P＜0.01);与高频组相比,低频组的Mankin评分,JNK、NF-κB p65mRNA和蛋白质表达均显著降低(P＜0.05,P＜0.01),而SOX9mRNA和蛋白质表达则显著升高(P＜0.05,P＜0.01)。结论: 不同频率的振动训练对早期膝骨关节炎关节软骨可表现出不同程度的修复效应,低频振动训练软骨修复优于高频振动。可能通过下调关节软骨JNK/NF-κB表达,提高SOX9活性来调控胶原合成。

关键词: 膝骨性关节炎, 振动训练, C-Jun 氨基端激酶, 核因子-κB, 性别决定区Y框蛋白9 , 大鼠

Abstract: Objective: To investigate the repair effect and JNK/NF-κB,SOX9 mechanisms of vibration exercise with different frequencies on articular cartilage in rats with early knee osteoarthritis. Methods: Forty-eight adult male SD rats were randomly divided into six groups(n=8):model control group(MC),high frequency vibration group 1 (GP₁,60 Hz),high frequency vibration 2 group (GP₂,40 Hz),medium frequency vibration group (ZP,20 Hz),minor frequency group(DP,10 Hz)and normal control group(NC). Except for NC group,the rats in each group were made into early knee osteoarthritis model after six weeks of knee joint cavity injection of papain solution and 2% mixture l-cysteine on the 1^st,4^th and 7^thday. Each exercise group was subjected vibration to 40 minutes a day with amplitude of 2～5 mm and 5 days a week. Four weeks later, the articular cartilage of the lateral femoral condyle of the both back leg knee joints were detected by HE staining,serine O staining and Mankin scores for morphological observation. The expression levels of JNK,NF-κB p65 and Sox9 mRNA in articular cartilage of the medial femoral condyle were detected by RT-qPCR,and the protein expressions of JNK,NF-κB p65 and Sox9 were detected by Western blot. Results: Compared with the NC group,the Mankin score in other groups was significantly higher (P＜0.01). Compared with the MC group,the Mankin score of each vibration group was significantly lower(P＜0.05),the mRNA and protein expressions of JNK and NF-κB p65 in each vibration training group were significantly lower (P＜0.01),the expressions of Sox9 mRNA and protein in vibration training group were increased significantly (P＜0.01). Compared with the higher frequency group,the Mankin score,the mRNA and protein expressions of JNK and NF-κB p65 of lower frequency group were significantly lower (P＜0.05 or P＜0.01). But the expressions of Sox9 mRNA and protein were significantly higher (P＜ 0.05 or P＜0.01). Conclusion: Vibration exercise of different frequencies may present varying degrees of cartilage repair impact in rats with early knee osteoarthritis,and the cartilage repair by low-frequency vibration training is better than that by high-frequency vibration. This can be one of the mechanisms on controlling collagen synthesis by down-regulating JNK/NF-κB expression and increasing SOX9 activity of OA articular cartilage.

Key words: knee osteoarthritis, vibration exercise, c-Jun N-termianl kinase, nuclear factor kappa B, sex determining region Y-box 9, rats

中图分类号:

R493

汪宗保, 王连, 柳奇奇, 杨永晖, 刘攀, 李斯亮, 姚长风. 不同频率的振动训练对大鼠早期膝骨关节炎关节软骨的修复作用及其JNK/NF-κB、SOX9机制^*[J]. 中国应用生理学杂志, 2022, 38(1): 41-46.

WANG Zong-bao, WANG Lian, LIU Qi-qi, YANG Yong-hui, LIU Pan, LI Si-liang, YAO Chang-feng. Repair impact of vibration exercise with different frequencies on articular cartilage of rats with early knee osteoarthritis and its JNK/NF-κB, SOX9 mechanisms[J]. CJAP, 2022, 38(1): 41-46.

参考文献

[1] 李刚, 籍胤玺, 高毅, 等. 帕瑞昔布对骨关节炎大鼠软骨细胞的影响[J]. 中国应用生理学杂志, 2020, 36(6): 652-655.
[2] 王朴, 季侨丹, 刘遄, 等. 全身振动治疗对膝骨性关节炎作用效果的研究进展[J]. 中国康复医学杂志, 2016, 31(3): 362-366.
[3] Zhang X, Yang Y, Li X, et al. Alterations of autophagy in knee cartilage by treatment with treadmill exercise in a rat osteoarthritis model[J]. Int J Mol Med, 2019, 43: 336-344.
[4] 陈伟达, 石玮, 杨继国, 等. 川续断总皂苷调节miR-19a对大鼠骨关节炎软骨细胞 SOX9/NF-кB信号通路的影响[J]. 中国药师, 2020, 23(5): 824-829.
[5] 陈臻浩, 赵广雷, 石晶晟, 等. Sox9对软骨细胞分化和基质产生的调控机制[J]. 复旦学报(医学版), 2019, 46(6): 824-828.
[6] 王朴, 魏晓霏, 阳筱甜, 等. 全身振动治疗对免膝骨关节炎早期软骨下骨结构与功能重塑的影响[J]. 四川大学学报(医学版), 2014, 45(1): 111-115.
[7] Li Z1, Tan C, Wu Y, et al. Whole-body vibration and resistance exercise prevent long-term hindlimb unloading-induced bone loss: independent and interactive effects[J]. Eur J Appl Physiol, 2012, 112(11): 3743-3753.
[8] 汪宗保, 廖威明, 徐亚林, 等.木瓜蛋白酶诱导大鼠膝早期骨关节炎软骨表面的电镜扫描[J].中国组织工程研究杂志, 2014, 18(2): 177-182.
[9] Ma N, Wang TT, Bie LY, et al. Comparison of the effects of exercise with chondroitin sulfate on knee osteoarthritis in rabbits[J]. J Orthop Surg Res, 2018, 13(1): 16.
[10] Rojas-Ortega M, Cruz R, Vega-López MA, et al. Exercise modulates the expression of IL-1β and IL-10 in the articular cartilage of normal and osteoarthritis-induced rats[J]. Pathol Res Pract, 2015, 211(6): 435-443.
[11] 黄吉利, 罗明, 潘捷, 等. 持续被动运动对兔骨关节炎软骨细胞凋亡相关基因P53、caspase-3、Bcl-2和Bax的影响[J]. 中国老年学杂志, 2016, 46(10): 1233-1236.
[12] 魏晓霏, 王补, 刘遄, 等. 全身振动对兔膝骨性关节炎关节软骨和软骨下骨的影响[J]. 中国康复医学杂志, 2018, 33(11): 1261-1267.
[13] Chen L, Loua YT, Pan ZQ, et al. Treadmill and wheel exercise protect against JNK/NF-kB induced inflammation in experimental models of knee osteoarthritis[J]. Biochem Biophys Res Commun, 2020, 523(1): 117-122.
[14] Tang G, Minemoto Y, Dibling B, et al. Inhibition of JNK activation through NF-kappaB target genes[J]. Nature, 2001, 414(6861): 313-317.
[15] Bell DM, Leung KK, Wheatley SC, et al. SOX9 directly regulates the type-II collagen gene[J]. Nat Genet, 1997, 16(2): 174-178.
[16] Kou I, Ikegawa S. SOX9-dependent and -independent transcriptional regulation of human cartilage link protein[J]. J Biol Chem, 2004, 279: 50942-50948.
[17] Rockel JS, Kudirka JC, Guzi AJ, et al. Regulation of Sox9 activity by crosstalk with nuclear factor-kappaB and retinoic acid receptors[J]. Arthritis Res Ther, 2008, 10(2): R3.
[18] Lefebvre V, Angelozzi M, Haseeb A. SOX9 in cartilage development and disease[J]. Curr Opin Cell Biol, 2019, 61: 39-47.

不同频率的振动训练对大鼠早期膝骨关节炎关节软骨的修复作用及其JNK/NF-κB、SOX9机制^*

Repair impact of vibration exercise with different frequencies on articular cartilage of rats with early knee osteoarthritis and its JNK/NF-κB, SOX9 mechanisms

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	徐畅, 吴晓玲, 程凯, 李娜. 完全弗氏佐剂诱导大鼠慢性骨盆疼痛综合征炎性痛模型的制备^*[J]. 中国应用生理学杂志, 2022, 38(1): 6-10.
[2]	郭亚净, 任静, 刘寒, 李亭亭, 张帅, 王洪. MCC950对脑出血大鼠神经损伤的作用^*[J]. 中国应用生理学杂志, 2022, 38(1): 11-16.
[3]	吕栋辉, 安方玉, 颜春鲁, 李海龙, 王嘉玉, 石瑶, 袁灵青, 赵延真, 杨建新, 郭丹, 颉旺军. 中风胶囊对脑缺血/再灌注损伤模型鼠脑组织自噬相关蛋白表达的影响^*[J]. 中国应用生理学杂志, 2022, 38(1): 25-31.
[4]	张媛, 盛蕾, 刘小玮, 韦娟, 刘秀娟, 张念云, 王子艺. 不同运动方式对肥胖大鼠肝脏脂质沉积及FGF21分泌的影响^*[J]. 中国应用生理学杂志, 2022, 38(1): 47-52.
[5]	李少情, 张青山, 赵婷, 白婷婷, 宣丽颖, 王羽, 赵明. 黄芪注射液对缺血性心肌病大鼠心肌的保护作用^*[J]. 中国应用生理学杂志, 2022, 38(1): 75-78.
[6]	张利娜, 杜相欣, 张雨彤, 郭霞, 郝娜, 赵欣, 张宇. 微丝电极阵列的参考电极内置与外置的对比研究^*[J]. 中国应用生理学杂志, 2022, 38(1): 85-90.
[7]	张雨彤, 赵欣, 尹艺儒, 吴淑芬, 张利娜, 杜相欣, 张宇. 老年大鼠脊髓小胶质细胞分选新方法及功能特征观察^*[J]. 中国应用生理学杂志, 2022, 38(1): 91-96.
[8]	汤娟, 柯洁, 付裕, 何晓伟, 李先伟. Ⅱ型肺泡上皮细胞间质转分化中的Notch-1/ Twist-1信号通路机制^*[J]. 中国应用生理学杂志, 2021, 37(6): 577-583.
[9]	齐书妍, 黄华, 李永坤, 裴林国, 张文娟. 莪术醇对非酒精性脂肪性肝大鼠肝功能和肝纤维化的影响及机制^*[J]. 中国应用生理学杂志, 2021, 37(6): 611-615.
[10]	朱海东, 吕长坤, 马菲菲. 牛磺酸上调基因1(TUG1)对肝纤维化的作用及其机制^*[J]. 中国应用生理学杂志, 2021, 37(6): 616-621.
[11]	吴江立, 王晓影, 代成, 李睿嘉, 张越, 孙佳欢, 李爱英. 归脾汤对大鼠心肌缺血的干预作用^*[J]. 中国应用生理学杂志, 2021, 37(6): 694-698.
[12]	涂容芳, 何振华, 谭小武, 陈哲, 曾赛丽, 刘莎, 贾远航, 李雪花. 辛伐他汀对大鼠肺纤维化及其内皮间质变过程的影响^*[J]. 中国应用生理学杂志, 2021, 37(5): 454-459.
[13]	黄燕云, 高洁, 张利娜, 杜相欣, 张雨彤, 郭霞, 郝娜, 李建国, 张宇. 利用全细胞膜片钳技术记录大鼠脑片NMDA电流的研究^*[J]. 中国应用生理学杂志, 2021, 37(5): 459-462.
[14]	丁茂鹏, 韦凌霞, 王志旺, 程小丽, 庞亚蓉. 鳖甲育肝颗粒对复合因素所致肝纤维化大鼠TGF-β1/Smads通路的影响^*[J]. 中国应用生理学杂志, 2021, 37(5): 463-467.
[15]	吴经纬, 王一蓉, 陈伟, 刘文锋. 不同训练方式对大鼠骨骼肌纤维类型转化和CaMK II/MEF2传导途径的影响^*[J]. 中国应用生理学杂志, 2021, 37(5): 470-474.