不同运动方式对肥胖大鼠肝脏脂质沉积及FGF21分泌的影响*

doi:10.12047/j.cjap.6209.2022.009

中国应用生理学杂志 ›› 2022, Vol. 38 ›› Issue (1): 47-52.doi: 10.12047/j.cjap.6209.2022.009

不同运动方式对肥胖大鼠肝脏脂质沉积及FGF21分泌的影响^*

张媛^1,2, 盛蕾^1△, 刘小玮^1,3, 韦娟¹, 刘秀娟¹, 张念云¹, 王子艺¹

1.南京体育学院运动健康学院, 江苏南京 210014;
2.上海交通大学系统生物医学研究院运动转化医学中心, 上海 200240;
3.无锡惠山康复医院, 江苏无锡 214100

收稿日期:2021-05-13 修回日期:2022-01-03 出版日期:2022-01-28 发布日期:2022-05-30
通讯作者: ^△Tel: 19962033918; E-mail: 735888670@qq.com
基金资助:
* 国家自然科学基金(青年项目)(32000839); 江苏省自然科学基金(面上项目)(BK20191473);中国博士后基金项目(2018M641990);江苏省“青蓝工程”资助(苏教师函[2021]11号);江苏省高校学校自然科学基金面上项目(18KJD310001)

Effects of different exercise on liver lipid accumulation and FGF21 secretion in obese rats

ZHANG Yuan^1,2, SHENG Lei^1△, LIU Xiao-wei^1,3, WEI Juan¹, LIU Xiu-juan¹, ZHANG Nian-yun¹, WANG Zi-yi¹

1. School of Sports and Health, Nanjing Sport Institute, Nanjing 210014;
2. The Key Laboratory of Systems Biomedicine, Ministry of Education, and the Exercise Translational Medicine Centre, Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240;
3. Huishan District Rehabilitation Hospital, Wuxi 214100, China

Received:2021-05-13 Revised:2022-01-03 Online:2022-01-28 Published:2022-05-30

摘要/Abstract

摘要： 目的: 研究持续性运动训练(CT)与高强度间歇运动训练(HIIT)对正常和肥胖大鼠血清和肝脏FGF21蛋白含量及肝脏脂肪代谢的影响。方法: 雄性SD大鼠随机分为两组:普通饲料及45%高脂饲料喂养,8周后以普通饲料喂养,大鼠体重增加20%为肥胖造模成功标准。将正常大鼠随机分为正常安静组(LC)、正常高强度间歇运动训练组(LHI)、正常持续性运动训练组(LCT),肥胖大鼠随机分为肥胖安静组(OC)、肥胖高强度间歇运动训练组(OHI)及肥胖持续性运动训练组(OCT),每组10只,运动干预组大鼠进行8周不同方式负重游泳运动训练干预,末次运动干预间隔24 h后取血液检测血清炎症因子、FGF21水平,取肝脏组织检测脂质含量、脂代谢酶含量及FGF21表达水平。结果: 与LC组比较,OC组大鼠体重、血清炎症因子、肝脏甘油三酯(TG)含量显著增高(P＜0.05),LHI组肝脏TG含量显著降低,LCT组肝脏FGF21表达水平显著增高(P＜0.05)。与OC组比较,OHI组大鼠肝脏TG含量显著降低(P＜0.05),线粒体CPT-1β、β-HAD酶含量显著升高(P＜0.05),OCT组大鼠肝脏LPL、FAT/CD36酶含量显著增高,血清、肝脏FGF21水平均显著上升(P＜0.05)。结论: 两种运动方式均能降低正常、肥胖大鼠体重及肥胖大鼠肝脏脂质沉积现象,其中HIIT上调线粒体脂肪氧化水平,显著降低正常、肥胖大鼠肝脏TG含量,而CT通过提高正常、肥胖大鼠肝脏FGF21蛋白表达及血清FGF21水平,促进肝脏摄取脂肪酸,对缓解肥胖大鼠肝脏脂质沉积效果有限。

关键词: 肥胖, 大鼠, 高强度间歇运动, 持续性运动, 肝脏脂代谢, 成纤维细胞生长因子21

Abstract: Objective: To investigate the effects of continuous exercise training (CT) and high-intensity interval exercise training (HIIT) on liver lipid metabolism and the correlation of the level of fibroblast growth factor 21(FGF21) in serum and liver tissues. Methods: Male SD rats were randomly divided into normal diet group (N) and obesity model group (H) after 1 week of adaptive feeding. Rats in the obesity model group were fed with 45% high-fat diet for about 8 weeks, and 20% weight increase compared with normal rats was considered as obesity. The rats were divided into normal diet control group (LC), normal diet HIIT group (LHI), normal diet CT group (LCT), High fat diet-induced obese control group (OC), obese HIIT group (OHI), and obese CT group (OCT) (n=10). Exercised rats were given weight-bearing swimming training intervention for 8 weeks. Blood samples were collected at least 24h after the last exercise intervention to detect the serum levels of inflammatory factors and FGF21. Liver tissue samples were collected to detect the lipid content, lipid metabolic enzyme content and FGF21 expression level. Results: Compared with LC group, the body weight, serum inflammatory factors levels and hepatic triglyceride content were increased significantly (P＜0.05). Hepatic triglyceride content was downregulated in LHI group and FGF21 expression level was enhanced in LCT group (P＜0.05). Compared with OC group, the body weight and hepatic triglyceride content were decreased significantly (P＜0.05), mitochondrial CPT-1β and β-HAD enzyme contents in liver were increased significantly (P＜0.05) in OHI group, the contents of LPL and FAT/CD36 enzyme in liver and the levels of FGF21 in serum and liver of OCT group were increased significantly (P＜0.05). Conclusion: Both exercise modes can reduce the body weight in normal and obese rats, and lipid deposition in the liver of obese rats. HIIT has a more significant effect on alleviating liver lipid deposition in obese rats by upregulating mitochondrial lipid oxidation level in normal and obese rats. CT improves the levels of FGF21 in serum and liver tissues of normal and obese rats, enhances enzyme contents that involved in fatty acids uptake to the liver, which has limited effect on alleviating lipid deposition in liver of obese rats.

Key words: obesity, rats, high-intensity interval exercise, continuous exercise, liver lipid metabolism, fibroblast growth factor 21

中图分类号:

G804.2

张媛, 盛蕾, 刘小玮, 韦娟, 刘秀娟, 张念云, 王子艺. 不同运动方式对肥胖大鼠肝脏脂质沉积及FGF21分泌的影响^*[J]. 中国应用生理学杂志, 2022, 38(1): 47-52.

ZHANG Yuan, SHENG Lei, LIU Xiao-wei, WEI Juan, LIU Xiu-juan, ZHANG Nian-yun, WANG Zi-yi. Effects of different exercise on liver lipid accumulation and FGF21 secretion in obese rats[J]. CJAP, 2022, 38(1): 47-52.

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不同运动方式对肥胖大鼠肝脏脂质沉积及FGF21分泌的影响^*

Effects of different exercise on liver lipid accumulation and FGF21 secretion in obese rats

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