Synaptotagmin 1基因敲除对小鼠行为的影响*

doi:10.12047/j.cjap.6233.2022.034

中国应用生理学杂志 ›› 2022, Vol. 38 ›› Issue (2): 97-101.doi: 10.12047/j.cjap.6233.2022.034

• 研究论文 • 下一篇

Synaptotagmin 1基因敲除对小鼠行为的影响^*

武南南¹, 封芮芮¹, 张宇¹, 李光², 曹济民¹, 殷丽天^1△

1.山西医科大学生理学系, 细胞生理学教育部重点实验室, 细胞生理学山西省重点实验室, 太原 030001;
2.西南医科大学心血管医学研究所, 医学电生理学教育部重点实验室, 四川泸州 646000

收稿日期:2021-12-03 修回日期:2022-03-07 出版日期:2022-03-28 发布日期:2022-08-29
通讯作者: ^△Tel: 13934509369; E-mail: yinlitian@126.com
基金资助:
^*国家自然科学基金(81601167,81670313,82170523);山西省回国留学人员科研资助项目(2020-080);山西省基础研究计划面上项目(20210302123304);山西省“1331工程”提质增效建设计划项目(1331KFC);山西省优秀研究生创新项目(2021Y407)

Effects of Synaptotagmin1 gene knockout on the behavior of mice

WU Nan-nan¹, FENG Rui-rui¹, ZHANG Yu¹, LI Guang², CAO Ji-min¹, YIN Li-tian^1△

1. Department of Physiology, Shanxi Medical University, Ministry of Education Key Laboratory of Cell Physiology, Shanxi Provincial Key Laboratory of Cell Physiology, Taiyuan 030001;
2. Institute of Cardiovascular Medicine, Southwest Medical University, Ministry of Education Key Laboratory of Medical Electrophysiology, Luzhou 646000, China

Received:2021-12-03 Revised:2022-03-07 Online:2022-03-28 Published:2022-08-29

摘要/Abstract

摘要： 目的: 研究Synaptotagmin 1基因敲除(Syt1^+/-)对小鼠情绪行为的影响并初步探讨其可能机制。方法: 选取8周龄雄性Syt1^+/-小鼠及同窝野生型(WT)小鼠各5只,采用免疫荧光染色方法观察小鼠前额叶皮层、海马、杏仁核、伏隔核、纹状体和腹侧被盖区等6个脑区中Syt1的表达;选用8周龄雄性Syt1^+/-小鼠9只,以及WT小鼠10只为对照,通过旷场实验、高架十字迷宫实验和强迫游泳实验检测比较成年Syt1^+/-小鼠和WT小鼠的焦虑样行为;另选用8周龄雄性Syt1^+/-小鼠及WT小鼠各5只,检测小鼠前额叶皮层、海马和杏仁核的谷氨酸含量。结果: 与WT小鼠相比,Syt1^+/-小鼠在前额叶皮层、海马、杏仁核、伏隔核、纹状体和腹侧被盖区Syt1阳性细胞数目显著减少(P＜0.01);Syt1^+/-小鼠在旷场中总移动距离显著减少(P＜0.01),并更偏爱在外周区域活动(P＜0.01),对中心区域的探索欲望显著下降(P＜0.01);Syt1^+/-小鼠更偏好待在封闭安全环境中(P＜0.01),开臂探索次数(P＜0.05)和在其中运动的时间显著减少(P＜0.01);Syt1^+/-小鼠在强迫游泳实验中不动时间明显增加(P＜0.01);同时,Syt1^+/-小鼠杏仁核中谷氨酸的含量显著增加(P＜0.01)。结论: Syt1基因敲除可以引起小鼠显著的焦虑样行为,推测与杏仁核中谷氨酸含量增加有关。

关键词: Synaptotagmin 1, 焦虑, 谷氨酸, 小鼠

Abstract: Objective: To study the effects of Synaptotagmin1 gene knockout (Syt1^+/-) on emotional behavior in mice and explore its possible mechanisms. Methods: Five 8-week-old male Syt1^+/-mice and five wild-type (WT) mice in the same litter were selected. The expressions of Syt1 in 6 mice brain regions of prelimbic cortex (PL), hippocampus (HIP), amygdala (AMY), accumbens nucleus (ACB), caudoputamen (CP) and ventral tegmental area (VTA) were detected by Immunofluorescence staining. Nine 8-week-old male Syt1^+/-mice and ten WT mice were selected as controls. The anxiety-like behaviors of adult Syt1^+/- mice and WT mice were detected by open field test, elevated plus maze test and forced swim test. In addition, five 8-week-old male Syt1^+/-mice and five WT mice were selected to detect the glutamate content in prelimbic cortex, hippocampus and amygdala. Results: Compared with WT mice, the number of Syt1 positive cells in PL, HIP, AMY, ACB, CP and VTA were decreased significantly in Syt1^+/- mice (P＜0.01); Syt1^+/- mice had less total movement distance in open field test (P＜0.01), more preference for peripheral area (P＜0.01) and less desire to explore the central platform (P＜0.01), while Syt1^+/- mice preferred to stay in a closed and safe environment (P＜0.01); the number (P＜0.05) and the time spent in open-arm explorations (P＜0.01) were reduced significantly; the immobile time of Syt1^+/- mice was increased in the forced swim test (P＜0.01). Meanwhile, the concentration of glutamate in the amygdala of Syt1^+/- mice was increased significantly (P＜0.01). Conclusion: Syt1 gene knockout leads to significant anxiety-like behavior in mice, which is deduced that related to the increase of glutamate content in the amygdala.

Key words: Synaptotagmin 1, anxiety, glutamate, mouse

中图分类号:

R338

武南南, 封芮芮, 张宇, 李光, 曹济民, 殷丽天. Synaptotagmin 1基因敲除对小鼠行为的影响^*[J]. 中国应用生理学杂志, 2022, 38(2): 97-101.

WU Nan-nan, FENG Rui-rui, ZHANG Yu, LI Guang, CAO Ji-min, YIN Li-tian. Effects of Synaptotagmin1 gene knockout on the behavior of mice[J]. CJAP, 2022, 38(2): 97-101.

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Synaptotagmin 1基因敲除对小鼠行为的影响^*

Effects of Synaptotagmin1 gene knockout on the behavior of mice

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