TOFA对人食管鳞癌Eca109和KYSE-450细胞生长的影响*

doi:10.12047/j.cjap.6236.2022.060

中国应用生理学杂志 ›› 2022, Vol. 38 ›› Issue (4): 317-321.doi: 10.12047/j.cjap.6236.2022.060

TOFA对人食管鳞癌Eca109和KYSE-450细胞生长的影响^*

谷城威^1,2, 钱河^1,2, 刘玉珍^1,2,3, 赵宝生^1,2△

1.新乡医学院第一附属医院胸外科, 河南新乡 453100;
2.新乡医学院食管癌研究所, 河南新乡 453100;
3.新乡医学院第一附属医院生命科学研究中心, 河南新乡 453100

收稿日期:2021-12-03 修回日期:2021-12-03 出版日期:2022-07-28 发布日期:2022-11-23
通讯作者: ^△Tel: 13903732289; E-mail: drbszhao@xxmu.edu.cn
基金资助:
^*新乡市科技攻关计划项目(GG2020027); 河南省医学科技攻关计划省部共建重点项目(SBGJ202102188); 新乡医学院第一附属医院青年基金项目(QN-2019-A03); 新乡医学院研究生科研创新支持计划项目(YJSCX202002Z)

Effects of TOFA on growth of Eca109 and KYSE-450 cells in human esophageal squamous cell carcinoma

GU Cheng-wei^1,2, QIAN He^1,2, LIU Yu-zhen^1,2,3, ZHAO Bao-sheng^1,2△

1. Department of Thoracic Surgery, the First Affiliated Hospital of Xinxiang, Xinxiang 453100, China;
2. Esophageal Cancer Institute of Xinxiang Medical University, Xinxiang 453100, China;
3. Life Science Research Center, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang 453100, China

Received:2021-12-03 Revised:2021-12-03 Online:2022-07-28 Published:2022-11-23

摘要/Abstract

摘要： 目的: 探讨5-十四烷氧基-2-呋喃酸(TOFA)对人食管鳞癌(ESCC)细胞Eca109和KYSE-450细胞增殖、周期和凋亡的影响。方法: 将Eca-109细胞和KYSE-450细胞分为对照组(DMSO)和实验组(TOFA),细胞(4×10³ cells/100 μl)接种于96孔板中,每个浓度设置5个复孔,培养24 h后,给予DMSO(对照)和不同浓度(1、3、5、10 μg/ml)TOFA处理,继续培养24、48和72 h;MTT检测细胞增殖,流式细胞术检测细胞周期和凋亡,Western blot检测p21、Cleaved caspase-3表达水平及p-AKT、p-mTOR、p-4EBP1修饰水平,专用试剂盒检测细胞内游离脂肪酸。结果: 与DMSO组比较,TOFA以浓度和时间依赖性方式抑制Eca109和KYSE-450细胞增殖(P均<0.05),处理48 h的IC₅₀分别为4.65和3.93 μg / ml;实验组细胞G2 / M 期细胞百分比增加,细胞凋亡率增高,p21、Cleaved caspase-3蛋白表达水平上调(P均<0.05),p-AKT、p-mTOR、p-4EBP1修饰水平下调(P均<0.05)。结论: TOFA抑制人食管鳞癌细胞增殖、阻滞细胞周期并促进细胞凋亡,其机制可能与其抑制AKT/mTOR/4EBP1信号通路有关。

关键词: 食管鳞癌, 5-十四烷氧基-2-呋喃酸(TOFA), 增殖, 周期, 凋亡, 细胞培养

Abstract: Objective: To investigate the effects of 5-tetradecanoxy 2-furanic acid (TOFA) on cell proliferation, cell cycle and apoptosis of esophageal squamous cell carcinoma (ESCC) cells. Methods: Eca-109 cells and KYSE-450 cells were divided into control group (DMSO) and experimental group (TOFA), respectively. The cells (4×10³ cells/100 μl) were inoculated into 96-well plates with 5 multiple wells at each concentration. After 24 h culture, cells were treated with DMSO or different concentrations (1, 3, 5, 10 μg/ ml) of TOFA for 24, 48 and 72 h. Cell proliferation was detected by MTT, cell cycle and apoptosis were detected by flow cytometry, the expression levels of p21 and Cleaved caspase-3 and modification levels of p-Akt, p-mTOR and p-4EBP1 were detected by Western blot, and intracellular free fatty acids were detected by special kits. Results: MTT results showed that TOFA inhibited the proliferation of Eca109 and KYSE-450 cells in a concentration and time dependent manner (all P<0.05), with IC₅₀ of 4.65 μg/ml and 3.93 μg/ml for 48 h, respectively. Flow cytometry results showed that compared with DMSO group, the percentage of cells in G2/M phase was increased and the apoptosis rate was increased in the experimental group. Western blotting results showed that compared with DMSO group, p21 and Cleaved caspase-3 protein expression levels were up-regulated, and p-AKT, p-mTOR and p-4EBP1 protein expression levels were down-regulated (all P<0.05). Conclusion: TOFA inhibits the proliferation, blocks the cycle progression and promotes apoptosis of ESCC, the mechanism may be related to the AKT/mTOR/4EBP1 signaling pathway.

Key words: esophageal squamous cell carcinoma, 5-tetradecanoxy 2-furanic acid (TOFA), proliferation, cycle, apoptosis, cell culture

中图分类号:

R73-3

谷城威, 钱河, 刘玉珍, 赵宝生. TOFA对人食管鳞癌Eca109和KYSE-450细胞生长的影响^*[J]. 中国应用生理学杂志, 2022, 38(4): 317-321.

GU Cheng-wei, QIAN He, LIU Yu-zhen, ZHAO Bao-sheng. Effects of TOFA on growth of Eca109 and KYSE-450 cells in human esophageal squamous cell carcinoma[J]. CJAP, 2022, 38(4): 317-321.

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TOFA对人食管鳞癌Eca109和KYSE-450细胞生长的影响^*

Effects of TOFA on growth of Eca109 and KYSE-450 cells in human esophageal squamous cell carcinoma

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